The 4D6 cells were plated in 35-mm glass bottom dishes (Ibidi) in the 4D6 cell growth medium described earlier and were then transfected with various combinations of the wild-type and mutant FOXN1 constructs. Twenty-four hours after transfection, the culture medium was exchanged with Leibovitz 15 medium lacking phenol red (Thermo Fisher Scientific), and cells were imaged with a ZeissLSM 880 inverted confocal laser scanning microscope. A laser wavelength of 488 nm was used to excite GFP or EYFP. Laser power was kept 50 μW or below to avoid phototoxicity. FOXN1 condensates were counted using the spot counter plug-in of the Fiji software. Default values for spot size and fluorescence were used for this analysis.
Leibovitz 15 medium lacking phenol red
Manufactured by Thermo Fisher Scientific
Leibovitz 15 medium lacking phenol red is a cell culture medium formulation used to support the growth and maintenance of various cell types in vitro. This medium is similar to the standard Leibovitz 15 (L-15) formulation, but it does not contain the pH indicator dye phenol red. The omission of phenol red allows for the use of this medium in applications where the presence of the dye may interfere with certain experimental procedures or analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using leibovitz 15 medium lacking phenol red
1
Quantifying FOXN1 Condensates in 4D6 Cells
2
Imaging Cellular Localization of FOXN1 Variants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4D6 cells were plated in 35 mm glass bottom dishes (Ibidi) in the 4D6 cell growth medium described earlier and were then transfected with various combinations of the wild-type and mutant FOXN1 constructs. Twenty-four hours after transfection the culture medium was exchanged with Leibovitz 15 medium lacking phenol red (Thermo Fisher Scientific) and cells were imaged with a ZeissLSM 880 inverted confocal laser scanning microscope. A laser wavelength of 488 nm wave length was used to excite GFP or EYFP. Laser power was kept 50 µW or below to avoid phototoxicity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!