The samples (20 mg) were extracted with 1 mL of ultrapure water. The mixed solution was subjected to ultrasound for 30 min and then centrifuged for 10 min at 14,000 rpm. All of the supernatant was filtered with a 0.1 μm injection syringe. The liquid was then diluted 20 times with ultrapure water. The concentration of saccharide compounds was determined using a Thermo Scientific Dionex ICS-5000+ ion chromatograph (Waltham, MA, USA). It was equipped with an SP single pump, EG eluent generator, AS-AP automatic sampler, DC electrochemical detector, and Chromeleon7.2 SR5 chromatographic data analytical software. The flow rate was 1 mL/min, and the injection volume was 10 µL. Ultrapure water and 200 mM NaOH (50% of NaOH was diluted with ultrapure water) were used as mobile phases A and B, respectively. The gradient elution procedure was as follows: 91% A, 0 min; 91% A, 18 min; 0% A, 21 min; 0% A, 31 min; 91% A, 32 min; and 91% A, 40 min.
Ics 5000 ion chromatograph
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICS-5000+ is an ion chromatograph designed for high-performance ion analysis. It features a modular design, allowing for the selection of appropriate components to meet specific analytical requirements.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ics 5000 ion chromatograph
1
Determination of Saccharide Compounds by Ion Chromatography
2
Ion Chromatography-MS for Metabolite Analysis
3
Quantitative Sulfate Determination in Cells
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Sulfate concentration was measured using a previously described method [21 (link), 53 (link)] with some modifications. In brief, 1-mL cell suspension samples treated by Na2SO3 or not were withdrawn from the 60-ml serum bottles at 0 or 12 h (Additional file 1 : Figure S3b) and were supersonically disrupted with a power of about 100 W by six repetitions of a 20-s pulse followed by 3-min incubation on ice. The homogenate was centrifuged at 12,000g for 5 min at 4 °C to remove unbroken cells and debris. Prior to measurement, the supernatant was filtered through a 0.22-µm filter. Subsequently, SO42− in the supernatant was determined using an ICS-5000 ion chromatograph (Dionex) equipped with an IonPac AG19 guard column (4 mm × 50 mm) and an IonPac AS19 separation column (4 mm × 250 mm), connected with a conductivity detector (Dionex).
4
Compost Pile Characterization Protocol
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The temperatures of the compost piles were measured with a Thermo Recorder RTW-30S (Espec). The pH of the aqueous extracts (solid-to-water ratio of 1:10, w/v) of the compost samples was measured with a SevenEasy pH metre S20K (METTLER TOLEDO). This dilution ratio was defined based on the water absorbance of the tested compost samples. The inorganic N (NO3－-N and NH4+-N) in the previously mentioned aqueous extracts was measured using an ICS 5000 ion chromatograph (DIONEX).
The TS, VS, TN and C/N ratio were measured according to Maeda and colleagues (2010 (link)).
The TS, VS, TN and C/N ratio were measured according to Maeda and colleagues (2010 (link)).
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