At 3 days following surgery, the total SOD activity, and MDA and lipid LPO levels in the hippocampus were assessed using a SOD assay kit (WST-8 method; cat. no. S0103; Beyotime Institute of Biotechnology), an MDA assay kit (cat. no. S0131; Beyotime Institute of Biotechnology) and an LPO kit (cat. no. JLC13854, Gelatins; Jiang Lanchun), respectively, according to the manufacturers' instructions.
Lpo kit
Manufactured by Beyotime
Sourced in China
The LPO kit is a lab equipment designed to measure the level of lipid peroxidation in biological samples. It provides a quantitative assessment of oxidative stress by determining the concentration of malondialdehyde, a marker of lipid peroxidation.
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Lab products found in correlation
2 protocols using lpo kit
1
Oxidative Stress Biomarkers in Hippocampus
2
Lipid Peroxidation Measurement in Cells
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After each group of cells received different treatments for 24 h, a lipid peroxidation assay (LPO) kit (Beyotime, Shanghai, China) was used following the instructions, aiming to measure the relative concentration of malondialdehyde (MDA) in the cell lysate.
The cells were planted in 12-well plates and subjected to various treatments for 24 h. The above cells were stained with the BODIPY C11 probe (Thermo Fisher, MA, USA). Finally, all the cells were digested, washed, and analyzed for FCM measurement (FACSCalibur, BD Biosciences, USA). The above-mentioned cells were also fixed and observed by fluorescence microscopy (Eclipse Ni, Nikon, Tokyo, Japan).
The cells were planted in 12-well plates and subjected to various treatments for 24 h. The above cells were stained with the BODIPY C11 probe (Thermo Fisher, MA, USA). Finally, all the cells were digested, washed, and analyzed for FCM measurement (FACSCalibur, BD Biosciences, USA). The above-mentioned cells were also fixed and observed by fluorescence microscopy (Eclipse Ni, Nikon, Tokyo, Japan).
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