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Chromium single cell 3 library construction kit v3

Manufactured by 10x Genomics
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The Chromium Single-Cell 3' Library Construction Kit v3 is a laboratory equipment product that enables the preparation of single-cell 3' gene expression libraries for analysis on Next Generation Sequencing platforms.

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Lab products found in correlation

Tapestation high sensitivity d1000, by Agilent Technologies (2 mentions) Chromium single cell b chip kit, by 10x Genomics (2 mentions) Novaseq 6000, by Illumina (2 mentions) Chromium controller, by 10x Genomics (2 mentions) Qubit dsdna hs assay kit, by Thermo Fisher Scientific (2 mentions) Chromium i7 multiplex kit, by 10x Genomics (1 mentions)

Spelling variants of this product

Chromium Single Cell 3′ Library (33 citations) Chromium Single Cell 3′ v3 (8 citations) Library Construction Kit (5 citations) Chromium Next GEM Single Cell 3’ Library Construction V3 Kit (3 citations)

2 protocols using chromium single cell 3 library construction kit v3

1

Rat Amygdala snRNA-seq Alignment Protocol

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snRNA-seq data used for the alignment metrics were obtained from rat amygdala using the Droplet-based Chromium Single-Cell 3′ solution (10x Genomics, v3 chemistry), as previously described.96 (link) Briefly, nuclei were isolated from frozen brain tissues and purified by flow cytometry. Sorted nuclei were counted and 12,000 were loaded onto a Chromium Controller (10x Genomics). Libraries were generated using the Chromium Single-Cell 3′ Library Construction Kit v3 (10x Genomics, 1000075) with the Chromium Single-Cell B Chip Kit (10x Genomics, 1000153) and the Chromium i7 Multiplex Kit for sample indexing (10x Genomics, 120262) according to manufacturer specifications. Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected. Final libraries were sequenced using the NovaSeq6000 (Illumina). Sequencing reads were aligned to the Rnor_6.0 and rat mRatBN7.2 genome assembly using Cell Ranger 3.1.0.
de Jong T.V., Pan Y., Rastas P., Munro D., Tutaj M., Akil H., Benner C., Chen D., Chitre A.S., Chow W., Colonna V., Dalgard C.L., Demos W.M., Doris P.A., Garrison E., Geurts A.M., Gunturkun H.M., Guryev V., Hourlier T., Howe K., Huang J., Kalbfleisch T., Kim P., Li L., Mahaffey S., Martin F.J., Mohammadi P., Ozel A.B., Polesskaya O., Pravenec M., Prins P., Sebat J., Smith J.R., Solberg Woods L.C., Tabakoff B., Tracey A., Uliano-Silva M., Villani F., Wang H., Sharp B.M., Telese F., Jiang Z., Saba L., Wang X., Murphy T.D., Palmer A.A., Kwitek A.E., Dwinell M.R., Williams R.W., Li J.Z, & Chen H. (2024). A revamped rat reference genome improves the discovery of genetic diversity in laboratory rats. Cell Genomics, 4(4), 100527.
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2

snRNA-seq Protocol for Rat Amygdala

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snRNA-seq data used for the alignment metrics were obtained from rat amygdala using the Droplet-based Chromium Single-Cell 39 solution (10x Genomics, v3 chemistry), as previously described 89 (link). Briefly, nuclei were isolated from frozen brain tissues and purified by flow cytometry. Sorted nuclei were counted and 12,000 were loaded onto a Chromium Controller (10x Genomics). Libraries were generated using the Chromium Single-Cell 3′ Library Construction Kit v3 (10x Genomics, 1000075) with the Chromium Single-Cell B Chip Kit (10x Genomics, 1000153) and the Chromium i7 Multiplex Kit for sample indexing (10x Genomics, 120262) according to manufacturer specifications. Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected. Final libraries were sequenced using the NovaSeq6000 (Illumina). Sequencing reads were aligned to the Rnor_6.0 and rat mRatBN7.2 genome assembly using Cell Ranger 3.1.0.
de Jong T.V., Pan Y., Rastas P., Munro D., Tutaj M., Akil H., Benner C., Chen D., Chitre A.S., Chow W., Colonna V., Dalgard C.L., Demos W.M., Doris P.A., Garrison E., Geurts A.M., Gunturkun H.M., Guryev V., Hourlier T., Howe K., Huang J., Kalbfleisch T., Kim P., Li L., Mahaffey S., Martin F.J., Mohammadi P., Ozel A.B., Polesskaya O., Pravenec M., Prins P., Sebat J., Smith J.R., Solberg Woods L.C., Tabakoff B., Tracey A., Uliano-Silva M., Villani F., Wang H., Sharp B.M., Telese F., Jiang Z., Saba L., Wang X., Murphy T.D., Palmer A.A., Kwitek A.E., Dwinell M.R., Williams R.W., Li J.Z, & Chen H. (2023). A revamped rat reference genome improves the discovery of genetic diversity in laboratory rats. bioRxiv.
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