Following an overnight fast (8 hours), blood samples were collected. Venous blood was drawn and sera were then collected after centrifugation at 3000×g for 15 min after coagulation. Serum was stored in the refrigerator at 4°C in aliquots until analysis. All laboratory variables were measured by a central laboratory that undergoes regular internal and external quality audits. A fully automatic biochemical analyzer (Roche, Mannheim, Germany) was used to determine the serum parameters. N-terminal pro-B type natriuretic peptide (NT-proBNP) was measured by using an immunoassay based on electrochemiluminescence with the Elecsys 1010/2010 System (Roche Diagnostics, Mannheim, Germany).
Elecsys 1010 2010 system
Manufactured by Roche
Sourced in Germany
The Elecsys 1010/2010 System is an automated immunoassay analyzer developed by Roche. It is designed for the quantitative determination of various analytes in human body fluids. The system utilizes electrochemiluminescence technology to perform immunoassay tests.
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Lab products found in correlation
2 protocols using elecsys 1010 2010 system
1
Serum Biomarker Measurement Protocol
2
Hemodynamic Measurements and WHO-FC in IPAH
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Patients with IPAH underwent right heart catheterization 1 week after blood collection, and baseline hemodynamic indicators such as heart rate, systolic blood pressure, diastolic blood pressure, mean right atrial pressure, mean pulmonary artery pressure, pulmonary vascular resistance, and cardiac output were measured as described previously (Radu et al., 2010 (link)). No changes in patient clinical status were observed between blood sampling and right heart catheterization. Cubic CO was measured by cold isotonic sodium chloride solution thermal dilution method. PVR was calculated by dividing the difference between mPAP and pulmonary artery wedge pressure by CO. The WHO-FC was determined according to the European PAH guidelines (Galiè et al., 2015 (link)). Electrochemiluminescence immunoassays based on the Elecsys 1010/2010 system (Roche Diagnostics GmbH, Mannheim, Germany) were used to quantify total plasma FSH concentration.
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