Both handling of the animals and surgical procedures were performed in accordance with the Animal Care and Use Committee approved by the Ethics Committee on Animal Experimentation at the University of Michigan (IACUC, protocol #PRO00008502). Engineered hydrogels with and without microparticles with antibiotics ( , ) were subcutaneously implanted into both sides of the dorsal of 6 wk. old Fischer 344 male rats (Envigo RMS, Inc., Oxford, MI, USA) weighing around 300–320 grams. Briefly, the rats were induced with isoflurane 4% (Piramal Critical Care Inc., Bethlehem, PA, USA) and maintenance of 2% buprenorphine (0.6 mg/kg) was injected subcutaneously. The surgical site was cleaned with povidone-iodine solution (Sigma-Aldrich). Four samples were implanted in the dorsum of the rats (four samples per group/animal).
Fischer 344 male rats
Manufactured by Inotiv
Sourced in United States
The Fischer 344 male rats are widely used laboratory animals in various research fields. These rats are known for their small size, docile temperament, and consistent physiological and behavioral characteristics. They serve as valuable models for studying a range of biological processes and disease conditions.
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Lab products found in correlation
2 protocols using fischer 344 male rats
1
Hydrogel Implants in Rat Dorsum
2
Aging Effects on Rat Gastrocnemius Muscle
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All animal experimental procedures were approved by Auburn University's Institutional Animal Care and Use Committee. Fischer 344 male rats aged 3, 12, and 24 mo (n ϭ 9 per age group) were purchased via Envigo Laboratories (Indianapolis, IN). Rats were housed two per cage at the Auburn University Biological Research Facility in quarters with constant 12-h light and 12-h dark cycles at ambient room temperature. Standard chow (24% protein, 58% carbohydrate, 18% fat; Teklad Global no. 2018 Diet, Envigo Laboratories) and tap water were provided to animals ad libitum.
The morning of the necropsies, rats were removed from the Biological Research Facility, transported to the Molecular and Applied Sciences Laboratory in the School of Kinesiology, and were then acclimated for ~3-4 h with ad libitum access to water only. After acclimation, rats were euthanized under CO 2 gas induction in a 2-liter chamber (VetEquip, Pleasanton, CA). Thereafter, total body mass was recorded, and right-leg gastrocnemius muscle was dissected out and weighed using an analytical scale with a sensitivity of 0.0001 g (Mettler-Toledo; Columbus, OH). Dissections on the right-leg gastrocnemius muscle were performed close to the origin and insertion sites, and any visible connective or fat tissue was removed. Tissues were then flash frozen in liquid nitrogen and stored at Ϫ80°C until analyses, described below.
The morning of the necropsies, rats were removed from the Biological Research Facility, transported to the Molecular and Applied Sciences Laboratory in the School of Kinesiology, and were then acclimated for ~3-4 h with ad libitum access to water only. After acclimation, rats were euthanized under CO 2 gas induction in a 2-liter chamber (VetEquip, Pleasanton, CA). Thereafter, total body mass was recorded, and right-leg gastrocnemius muscle was dissected out and weighed using an analytical scale with a sensitivity of 0.0001 g (Mettler-Toledo; Columbus, OH). Dissections on the right-leg gastrocnemius muscle were performed close to the origin and insertion sites, and any visible connective or fat tissue was removed. Tissues were then flash frozen in liquid nitrogen and stored at Ϫ80°C until analyses, described below.
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