Stercobilin hydrochloride

Manufactured by Frontier Scientific

Stercobilin hydrochloride is a chemical compound used as a reference standard in laboratory analysis. It is a dark reddish-brown crystalline powder that is soluble in water and organic solvents. The primary function of stercobilin hydrochloride is to serve as a reference material for the identification and quantification of stercobilin in various biological samples, such as urine and feces, during analytical procedures.

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Lab products found in correlation

Bilirubin, by Merck Group (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Hplc grade acetonitrile, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Oasis max 1 cc 30 mg extraction cartridges, by Waters Corporation (1 mentions) Hplc grade methanol, by Merck Group (1 mentions) Ammonium hydroxide, by Avantor (1 mentions)

2 protocols using stercobilin hydrochloride

Mass Spectrometry Reagent Preparation

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Methylene chloride (ACS grade) and methanol (HPLC grade) were from Fisher Scientific. Ethanol (200 proof) was from Decon Laboratories, Inc. Acetonitrile (HPLC grade) and bilirubin were from Sigma-Aldrich. Ammonium hydroxide and water (LC/MS grade) were from J. T. Baker. Alpha-4-cyanohydroxy cinnamic acid (CHCA) was from Fluka. Trifluoroacetic acid (TFA) was from Aldrich. Stercobilin hydrochloride was from Frontier Scientific (Logan, UT).

Yilmaz A., Rudolph H.L., Hurst J.J, & Wood T.D. (2015). High-Throughput Metabolic Profiling of Soybean Leaves by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Analytical chemistry, 88(2), 1188-1194.

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Synthesis and Stability of 18O-Labeled Stercobilin

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The following materials were used in the synthesis of ¹⁸O labeled stercobilin and determination of stability studies: stercobilin hydrochloride (Frontier Scientific, Logan, UT), isotopically labeled water (¹⁸O, 97%) (Cambridge Isotope Laboratories, Inc., Tewksbury, MA), methanol, HPLC grade ≥99.9% (Sigma-Aldrich, St. Louis, MO), acetonitrile, HPLC grade ≥99.9% (Sigma-Aldrich, St. Louis, MO), ammonium hydroxide (J.T. Baker, Central Valley, PA), trifluoroacetic acid (TFA) and formic acid (FA, Fisher Scientific, Fair Lawn, NJ), water filtered using Thermo Barnstead Nanodiamond™ filtration system (San Jose, CA), and Oasis © MAX 1cc 30mg extraction cartridges (Waters, Milford, MA).

Rudolph H.L., Sekera E.R, & Wood T.D. (2016). Stable 18O Labeling Method for Stercobilin and Other Bilins for Metabolomics. Rapid communications in mass spectrometry : RCM, 30(13), 1469-1474.

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