The amount of encapsulated Tan IIA in the NPs was evaluated by a direct method. One milliliter of the samples (1.0 mg/mL) was collected and diluted with methanol to 10 mL; demulsification was then performed by sonication (SB25-12-YDTD; Scientz, Ningbo, People’s Republic of China) for 15 minutes. The mobile phase was diluted to a constant volume of 10 mL, and 1 mL of the testing solution was filtered through a 0.45 μm organic membrane filter before being detected by high-performance liquid chromatography (LC-20A; Shimadzu, Kyoto, Japan). The mobile phase was a mixture of water and acetonitrile (15:85). The flow rate was set to 1 mL/min with a detection of 270 nm. Separation was achieved using a Platisil C18 column (150 mm ×4.6 mm, 5 μm; Dikma, Beijing, People’s Republic of China). The column temperature was 30°C. The encapsulation efficiency (EE%) was evaluated using the following formula:
Platisil c18 column
Manufactured by Dikma Technologies
The Platisil C18 column is a type of laboratory equipment used for chromatographic separation and analysis. It is designed with a C18 stationary phase, which is a common choice for reversed-phase liquid chromatography. The core function of the Platisil C18 column is to facilitate the separation and purification of a wide range of organic compounds, including pharmaceuticals, natural products, and other small molecules.
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2 protocols using platisil c18 column
1
Encapsulation Efficiency of Tan IIA in NPs
2
PAHs Extraction by Magnetic Fe3O4 Nanospheres
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The MSPE procedures for PAHs by Fe3O4 hollow nanospheres are as follows. Firstly, 5 mg of Fe3O4 hollow nanospheres was added into 20 mL of sample solution containing 100 ng/mL of the PAHs (200 μL) in a glass vial. The mixture was then ultrasonicated for 1 min and kept still for 30 min for extraction. After the extraction, the Fe3O4 hollow nanospheres with bound PAHs were separated using an external magnet and the supernatant was descanted. In order to desorption of PAHs from the sorbent, 400 μL of acetonitrile were added and sonicated for 1 min. After the magnetic separation, the desorbed solution was filtered for HPLC analysis. The extraction procedures on bare Fe3O4 nanoparticles were the same as Fe3O4 hollow nanospheres.
The chromatographic separation was performed on Dikma Platisil C18 column (25 × 4.6 mm i.d., 5 μm particle size). The temperature of HPLC separation was set at 30°C and the injection volume was 20 μL. A mobile phase of acetonitrilewater (85:15, v/v) was employed at a flow rate of 1 mL/min. The detection wavelength was set at 300 nm for exiting wavelength and 440 nm for emission wavelength.
The chromatographic separation was performed on Dikma Platisil C18 column (25 × 4.6 mm i.d., 5 μm particle size). The temperature of HPLC separation was set at 30°C and the injection volume was 20 μL. A mobile phase of acetonitrilewater (85:15, v/v) was employed at a flow rate of 1 mL/min. The detection wavelength was set at 300 nm for exiting wavelength and 440 nm for emission wavelength.
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