H2o lc ms grade

BPA (≥99%), the internal standard (IS) deuterated BPA-d16 (analytical standard), the derivatization reagent pyridine-3-sulfonyl (PS) chloride (≥95%), formic acid (FA, LC-MS grade), methanol (MeOH, LC-MS grade), and acetonitrile (ACN, LC-MS grade) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). H2O (LC-MS grade) was purchased from Biosolve (Valkenswaard, The Netherlands). Dimethylsulfoxide (DMSO), hydrochloric acid (HCl), sodium hydroxide (NaOH), and ammonium fluoride (NH4F) were purchased from Merck KGaA (Darmstadt, Germany). Oasis PRiME HLB (3 mL; 60 mg) extraction cartridges were purchased from Waters Corporation (Milford, MA, USA). AFFINIMIP SPE Bisphenols extraction cartridges were purchased from AFFINISEP (Petit-Couronne, France).

The following chemicals and reagents were used: BPA (≥99%), the internal standard (IS) deuterated BPA-d16 (analytical standard), the derivatization reagent pyridine-3sulfonyl (PS) chloride (≥95%), formic acid (FA, LC-MS grade), methanol (MeOH, LC-MS grade), and acetonitrile (ACN, LC-MS grade) (all from Sigma-Aldrich, Saint Louis, MO, USA), H2O (LC-MS grade, from Biosolve, Valkenswaard, The Netherlands), dimethylsulfoxide (DMSO), hydrochloric acid (HCl), sodium hydroxide (NaOH), and ammonium fluoride (NH4F) (all from Merck KGaA, Darmstadt, Germany). Solid-phase extraction was performed with Oasis PRiME HLB (3 mL; 60 mg; from Waters Corporation, Milford, MA, USA) and AFFINIMIP SPE Bisphenols (from AFFINISEP, Petit-Couronne, France) extraction cartridges.