Tissue sections of the ipsilesional striatum were harvested, homogenized, sonicated, and treated with protease/ phosphatase inhibitor cocktail (5872, Cell Signaling). The expression of cytokines was analyzed by the Proteome Pro ler Mouse Cytokine Array Panel A Kit (ARY006, R&D Systems, Boston, USA) from a total of 9 animals (n=3 for vehicle, n=3 for 0.3 mg/kg TAK-063, and n=3 for 3 mg/kg TAK-063), according to the manufacturer's recommendation. Array panels were visualized using the ChemiDoc MP System (1708280, Bio-Rad). Protein levels were analyzed densitometrically using an image analysis system (Image J), corrected with values determined on positive controls and expressed as relative values compared with vehicle treated group.
Proteome pro ler mouse cytokine array panel a kit
Manufactured by R&D Systems
Sourced in United States
The Proteome Profiler Mouse Cytokine Array Panel A Kit is a lab equipment product designed for the simultaneous detection and quantification of 40 different mouse cytokines, chemokines, and other related proteins in a single experiment. The kit utilizes a membrane-based antibody array format to enable parallel measurement of multiple analytes from a single sample.
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Lab products found in correlation
5 protocols using proteome pro ler mouse cytokine array panel a kit
1
Cytokine Expression Analysis in Striatum
2
Cytokine Expression Analysis in Striatum
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Tissue sections of the ipsilesional striatum were harvested, homogenized, sonicated, and treated with protease/ phosphatase inhibitor cocktail (5872, Cell Signaling). The expression of cytokines was analyzed by the Proteome Pro ler Mouse Cytokine Array Panel A Kit (ARY006, R&D Systems, Boston, USA) from a total of 9 animals (n=3 for vehicle, n=3 for 0.3 mg/kg TAK-063, and n=3 for 3 mg/kg TAK-063), according to the manufacturer's recommendation. Array panels were visualized using the ChemiDoc MP System (1708280, Bio-Rad). Protein levels were analyzed densitometrically using an image analysis system (Image J), corrected with values determined on positive controls and expressed as relative values compared with vehicle treated group.
3
Cytokine Profiling and Transcriptome Analysis
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The supernatant was analyzed using a Proteome Pro ler Mouse Cytokine Array Panel A Kit (R&D Systems; catalog number ARY006) at the baseline and after LPS stimuli according to the manufacturer's indications. Images were captured using a LAS 4000 (lmageQuant™) and analyzed using ImageJ software program.
2.14 RNA sequence and data analysis RNA quality was assessed with an Agilent 2100 bioanalyzer using an RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, Netherlands). The library construction was performed using a QuantSeq 3′-mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions. Highthroughput sequencing was performed as 75 single-end sequences using NextSeq 500 (Illumina, Inc., USA). QuantSeq 3′-mRNA-Seq reads were aligned using Bowtie2 (Langmead and Salzberg, 2012). Differentially expressed genes were determined based on the counts from unique and multiple alignments using coverage in Bedtools (Quinlan AR, 2010). The Read Count data were processed based on the quantile normalization method using Edge R within R (R Development Core Team, 2016) using Bioconductor (Gentleman et al., 2004). Gene classi cation was based on searches done using DAVID (http://david.abcc.ncifcrf.gov/) and Medline databases (http://www.ncbi.nlm.nih.gov/).
2.14 RNA sequence and data analysis RNA quality was assessed with an Agilent 2100 bioanalyzer using an RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, Netherlands). The library construction was performed using a QuantSeq 3′-mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions. Highthroughput sequencing was performed as 75 single-end sequences using NextSeq 500 (Illumina, Inc., USA). QuantSeq 3′-mRNA-Seq reads were aligned using Bowtie2 (Langmead and Salzberg, 2012). Differentially expressed genes were determined based on the counts from unique and multiple alignments using coverage in Bedtools (Quinlan AR, 2010). The Read Count data were processed based on the quantile normalization method using Edge R within R (R Development Core Team, 2016) using Bioconductor (Gentleman et al., 2004). Gene classi cation was based on searches done using DAVID (http://david.abcc.ncifcrf.gov/) and Medline databases (http://www.ncbi.nlm.nih.gov/).
4
Cytokine Expression Analysis in Striatum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections of the ipsilesional striatum were harvested, homogenized, sonicated, and treated with protease/ phosphatase inhibitor cocktail (5872, Cell Signaling). The expression of cytokines was analyzed by the Proteome Pro ler Mouse Cytokine Array Panel A Kit (ARY006, R&D Systems, Boston, USA) from a total of 9 animals (n=3 for vehicle, n=3 for 0.3 mg/kg TAK-063, and n=3 for 3 mg/kg TAK-063), according to the manufacturer's recommendation. Array panels were visualized using the ChemiDoc MP System (1708280, Bio-Rad). Protein levels were analyzed densitometrically using an image analysis system (Image J), corrected with values determined on positive controls and expressed as relative values compared with vehicle treated group.
5
Cytokine Expression Analysis in Striatum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections of the ipsilesional striatum were harvested, homogenized, sonicated, and treated with protease/ phosphatase inhibitor cocktail (5872, Cell Signaling). The expression of cytokines was analyzed by the Proteome Pro ler Mouse Cytokine Array Panel A Kit (ARY006, R&D Systems, Boston, USA) from a total of 9 animals (n=3 for vehicle, n=3 for 0.3 mg/kg TAK-063, and n=3 for 3 mg/kg TAK-063), according to the manufacturer's recommendation. Array panels were visualized using the ChemiDoc MP System (1708280, Bio-Rad). Protein levels were analyzed densitometrically using an image analysis system (Image J), corrected with values determined on positive controls and expressed as relative values compared with vehicle treated group.
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