Polyglutamine aggregates present in Q40 animals fed RNAi bacteria were counted using a fluorescence dissection stereomicroscope (Leica Microsystems). Twenty animals were counted per experiment and each experiment was repeated for at least 3 times. Punctae were counted with no size cut-off.
Fluorescence dissection stereomicroscope
Manufactured by Leica
The Fluorescence dissection stereomicroscope is a high-quality optical instrument designed for precise observation and examination of specimens. It provides a stereoscopic, three-dimensional view of the sample, allowing for detailed analysis and manipulation. The microscope is equipped with fluorescence illumination capabilities, enabling the visualization of fluorescently labeled samples.
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Lab products found in correlation
2 protocols using fluorescence dissection stereomicroscope
1
Quantifying Polyglutamine Aggregates
2
Germ Corpse Counting in C. elegans
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Synchronized young adult animals (AD2-3) grown on RNAi bacteria at 25º were stained with 75 µg/ml acridine orange (AO)(Sigma) for 1 hour, and transferred to fresh NGM agar plates for 3 hours to clear excess AO from their intestines. AO positive germ corpses present in the posterior gonads were counted using a fluorescence dissection stereomicroscope (Leica Microsystems), for each indicated condition 40 animals were counted. Irradiated animals were analyzed 24-36 hours post irradiation with the indicated doses (IBL-637 irradiator, CIS Biointernational).
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