Fluorescein goat anti mouse igg

Manufactured by Thermo Fisher Scientific

Sourced in United States

Fluorescein goat anti-mouse IgG is a secondary antibody labeled with the fluorescent dye fluorescein. It is designed to bind to mouse immunoglobulin G (IgG) antibodies, allowing for the detection and visualization of target proteins in various immunoassays and imaging applications.

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Close spelling variants of this product

Fluorescein isothiocyanate-conjugated goat anti-mouse IgG (3 citations) Mouse anti-IgG (2 citations) Fluorescein goat anti-mouse IgG (H+L) (2 citations) Goat anti-mouse IgG fluorescein isothiocyanate-conjugated antibodies (2 citations)

8 protocols using fluorescein goat anti mouse igg

BrdU Labeling and Immunodetection

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One hour before euthanasia, the animals received an intramuscular injection of 5-bromo-2 0 -deoxyuridine (BrdU, 10 mg/mL, 5 mL/kg; Sigma-Aldrich Corp., St. Louis, MO, USA), a DNA synthesis marker. Sections were deparaffinized and treated with 2 N HCl at 378C for 1 hour, then incubated with mouse monoclonal IgG anti-bromodeoxyuridine (anti-BrdU) (Agilent Technologies, Santa Clara, CA, USA) for 30 minutes at room temperature. The secondary antibody was fluorescein goat anti-mouse IgG (1:100; Molecular Probes, Leiden, The Netherlands) in Tris-buffered saline. Control sections were prepared by omission of the primary antibody.

Lorenzo-Martín E., Gallego-Muñoz P., Ibares-Frías L., Marcos S., Pérez-Merino P., Fernández I., Kochevar I.E, & Martínez-García M.C. (2018). Rose Bengal and Green Light Versus Riboflavin-UVA Cross-Linking: Corneal Wound Repair Response. Investigative ophthalmology & visual science, 59(12).

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BrdU Incorporation in Corneal Cells

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One hour before euthanasia, all animals received an intramuscular injection of 5-bromo-2'-deoxyuridine (BrdU, Sigma-Aldrich), a marker of DNA synthesis (10 mg/ml, 5 ml/kg). Paraffin-embedded sections were deparaffinized and treated with 2-N HCl for 1 hour and then incubated with mouse monoclonal IgG anti-BrdU (DakoCytomation, Carpinteria, CA, USA) for 30 minutes at room temperature. The secondary antibody was fluorescein goat anti-mouse IgG (1:100, Molecular Probes, Leiden, The Netherlands) in Tris-buffered saline. Control slides were prepared by omission of the primary antibody.
Using 20× magnification micrographs, epithelial and stromal BrdU-positive cells were counted in six equal areas (Fig. 1c): L1, limbus near the segment; R, area containing the segment; C, three equal areas of the central cornea; and L2, limbus far from the segment. The number of BrdUpositive cells in the three areas of the central cornea (labeled C in Fig. 1c) were averaged and compared with the other three areas (labeled L1, R, and L2 in Fig. 1c).

Ibares-Frías L., Gallego P., Cantalapiedra-Rodríguez R., Valsero M.C., Mar S., Merayo-Lloves J, & Martínez-García M.C. (2015). Tissue reaction after intrastromal corneal ring implantation in an experimental animal model. Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 253(7).

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BrdU Labeling for Cell Proliferation

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One hour before euthanasia, the animals received an intramuscular injection of 5-bromo-2´-deoxyuridine (BrdU, Sigma-Aldrich), a marker of DNA synthesis (10 mg/ml, 5 ml/kg). Sections were deparaffinized and treated with 2 N HCl for 1 hour, then incubated with mouse monoclonal IgG anti-BrdU (Dako, Cytomation Carpinteria, CA, USA) for 30 minutes at room temperature. The secondary antibody was fluorescein goat anti-mouse IgG (1:100, Molecular Probes, Leiden, The Netherlands) in Tris-buffered saline. In control slides, the primary antibody was omitted.

Ibares-Frías L., Gallego P., Cantalapiedra-Rodriguez R., Merayo-Lloves J, & Martínez-García M.C. (2016). Clinical, Refractive and Histological Reversibility of Corneal Additive Surgery in Deep Stroma in an Animal Model. Current eye research, 41(9).

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Immunostaining Protocol for Axonal Outgrowth Analysis

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After 48 h exposure to LA, 13-HODE or PGE2, cultures were fixed with 4% paraformaldehyde (Sigma-Aldrich) in phosphate-buffered saline (PBS) for 45 min, rinsed 3 times with PBS for 5 min and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) in PBS for 5 min. Permeabilized cells were blocked in 5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 1 h. Cells were then incubated overnight at 4 °C with the primary antibody anti-Tau1 (Millipore, Billerica, MA, USA) diluted 1/1000 in 5% BSA in PBS to selectively label axons. Cultures were washed 3 times with PBS and incubated for 1 h at room temperature with secondary antibody, fluorescein goat anti-mouse IgG (Invitrogen, Thermo Fisher Scientific) diluted 1:1000 in 5% BSA in PBS. Slides were mounted in Invitrogen Prolong Gold Antifade Reagent with DAPI (Invitrogen, Thermo Fisher Scientific) and images of immunostained neurons were captured from 4 separate dissection in an unbiased fashion using an automated high content imaging system (ImageXpress, Molecular Devices) (Dragunow 2008 (link)). Automated image analysis of axonal outgrowth utilized a cell scoring journal in MetaXpress software (Molecular Devices, version 5.3.0.5).

Hennebelle M., Morgan R.K., Sethi S., Zhang Z., Chen H., Grodzki A.C., Lein P.J, & Taha A.Y. (2019). Linoleic acid-derived metabolites constitute the majority of oxylipins in the rat pup brain and stimulate axonal growth in primary rat cortical neuron-glia co-cultures in a sex-dependent manner. Journal of neurochemistry, 152(2), 195-207.

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Measuring Extracellular ATP and Cell Surface Calreticulin

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A549 and B16F10 cells were transfected for 24 h and 48h. After incubation, Cells and supernatants were collected separately. The extracellular ATP content was measured with an ENLITEN®ATP Assay system Bioluminescence Detection Kit (Promega) according to the manufacture's instruction immediately. Cell surface expression of CRT was detected by flow cytometric analysis. The transfected cells were collected with trypsin and cell culture medium, stained using the Anti-hCalreticulin (0.025 µg/100 µl, R&D) and Fluorescein goat anti-mouse IgG (1:100, Invitrogen), analyzed by flow cytometry using AttuneTM NXT.

Yang J., Sun J., Zhu J., Du Y., Tan Y., Wei L., Zhao Y., Hou Q., Zhang Y., Sun Z., & Zuo C. (2022). Circular mRNA encoded PROTAC (RiboPROTAC) as a new platform for the degradation of intracellular therapeutic targets.

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Immunofluorescence Imaging of Viral Protein Localization

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HeLa cells grown on coverslips in 24-well-plates were transfected with 0.5 µg pCAGGS-P, 0.125 µg pCAGGS-N and increasing amounts of pCAGGS-M or 0.5 µg M mutants by Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). 24 h pt, cells were washed with cold PBS and fixed with 4% paraformaldehyde for 20 min. Then, the cells were permeabilised with 0.2% Triton X-100 for 20 min at room temperature. Blocking the permeated cells by 3% bovine serum albumin (BSA) in PBS for 0.5 h at room temperature and incubating the cells with mouse monoclonal anti-HA antibody (Sigma, St. Louis, MI, USA; 1:2000) and rabbit polyclonal anti-Myc antibody (Santa Cruz, CA, USA; 1:200) in 1% BSA at room temperature for 2 h. Then, the cells were washed with 1% BSA in PBS and incubated with the goat anti-mouse IgG fluorescein (Thermo; 1:200) and goat anti-rabbit IgG rhodamine (Thermo; 1:100) secondary antibody for 2 h at room temperature. After being washed three times with 1% BSA in PBS, the coverslips were turned over and the cells were incubated with a drop of DAPI (4′,6-diamidino-2-phenylindole)-contained Fluorshield for nuclear staining. Confocal images were collected by an Olympus confocal FV1000 microscope (Olympus, Tokyo, Japan).

Zhang S., Cheng Q., Luo C., Qin Y, & Chen M. (2018). Human Parainfluenza Virus Type 3 Matrix Protein Reduces Viral RNA Synthesis of HPIV3 by Regulating Inclusion Body Formation. Viruses, 10(3), 125.

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Antibody Acquisition and Reagent Details

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Mouse anti-TGN46 monoclonal Ab was purchased from Sigma-Aldrich. Mouse anti-Tom20 monoclonal Ab was purchased from BD Biosciences. Rabbit anti-Calnexin polyclonal antibodies (Abs) was purchased from Sigma-Aldrich. Rabbit anti-PDI polyclonal Ab was purchased from Abcam. Rabbit anti-LMNB1 Ab was purchased from ABclonal. Rabbit anti-PI4KA polyclonal Ab was purchased from ABclonal Technology. Rabbit anti-PI4B and anti-PI4K2B polyclonal Abs were purchased from Abcam. Rabbit anti-PI42A polyclonal Ab was purchased from Cell Signaling Technology. Mouse anti-HPIV3 HN monoclonal Ab was purchased from Abcam. Mouse anti-PI4P monoclonal Ab was purchased from Echelon Biosciences. Mouse anti-Flag monoclonal Ab, rabbit anti-Flag polyclonal Ab, and mouse anti-HA monoclonal Ab were purchased from Sigma-Aldrich. Mouse anti-Myc and anti-GAPDH monoclonal Abs were purchased from Santa Cruz Biotechnology, Inc. Goat anti-rabbit IgG Rhodamine and goat anti-mouse IgG Fluorescein were purchased from Thermo. DAPI was purchased from Sigma-Aldrich. PIK93 was purchased from Sigma-Aldrich and dissolved in dimethylsulfoxide (DMSO). Thapsigargin (Tg) was a gift from Y. Liu Lab (Wuhan University). Tunicamycin (Tu) was purchased from Med Chem Express (MCE) and dissolved in DMSO.

Li Z., Guo D., Qin Y, & Chen M. (2019). PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3. Cell Reports, 29(8), 2229-2242.e4.

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Antibody Profiling for Cell Analysis

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Rabbit anti-LC3B, anti-Atg5, anti-Atg7, anti-PINK1, anti-NDP52, anti-PA28, anti-PSMA3, anti-L7a, anti-RPL5 and anti-p62 polyclonal antibodies (Abs) were purchased from Cell Signaling Technology. Mouse anti-COXII monoclonal Ab was purchased from Abcam. Rabbit anti-TUFM polyclonal Ab, mouse anti-Flag monoclonal Ab, rabbit anti-Flag polyclonal Abs, and mouse anti-HA monoclonal Abs were purchased from Sigma Aldrich. Rabbit anti-Myc, anti-Beclin1, anti-GFP polyclonal Abs, mouse anti-Myc, anti-ubiquitin and anti-GAPDH monoclonal Abs were purchased from Santa Cruz Biotechnology Inc. Mouse anti-TIM23 and anti-TOM20 were purchased from BD Biosciences. Anti-HPIV3 HN monoclonal Ab was purchased from Abcam. Anti-GST polyclonal Ab was purchased from Amersham Bioscience. Rabbit anti-VISA polyclonal Ab was achieved from Shu HB Lab. Goat anti-rabbit IgG Rhodamine and goat anti-mouse IgG Fluorescein were purchased from Thermo. Goat anti-mouse IgG DyLight 350 was purchased from Abbkine. BAF and CCCP were purchased from Sigma Aldrich. ExRed was purchased from Zoman Biotechnology.

Ding B., Zhang L., Li Z., Zhong Y., Tang Q., Qin Y, & Chen M. (2017). The Matrix Protein of Human Parainfluenza Virus Type 3 Induces Mitophagy that Suppresses Interferon Responses. Cell host & microbe, 21(4).

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