Ika mag

After washing and drying, the powder was completely dissolved in 1 M HCl to determine the maximum release and loading efficiency. An aliquot was taken out and mixed with 700 µL of ethanol, 300 µL of 1 M HCl, and 50 µL of 0.02 M xanthyrol prior to the quantification with HPLC (RP-HPLC, Hitachi, Chiyoda, Japan).
Particle stability was determined by measuring drug release after incubating in phosphate buffer at pH 7.4. Additionally, 50 mg of particles were immersed in 10 mL of phosphate buffer and placed onto a shaker (IKAMAG^®, IKA Werke GmbH & Co. KG, Staufen im Breisgau, Germany), at a speed of 50 rpm. An aliquot (300 µL) was taken out after 1 h, 1 day, 1 week, and 2 weeks.

Samples were prepared by adding heated aqueous phase (75 °C) to the heated oil phase (70 °C) (Table 1) on thermostatic heating plate of magnetic stirrer IKA-MAG (IKA Werke, Staufen, Germany). A propeller rotary laboratory stirrer RW16 basic (IKA Werke) was used for stirring. At a temperature of 40 °C, herbal active substances (hemp oil and immortelle extract) were added into active cream sample (AC), and the stirring was continued until the preparation had cooled. The placebo cream (PAC) sample did contained neither hemp oil nor immortelle extract.

Samples were prepared by adding heated aqueous phase (75 °C) to the heated oil phase (70 °C) (Table 1) on thermostatic heating plate of magnetic stirrer IKA-MAG (IKA Werke, Staufen, Germany). Propeller rotary laboratory stirrer RW16 basic (IKA Werke, Germany) was used for stirring. At the temperature of 40 °C herbal active substances-wild bilberry leaves extract and wild bilberry seed oil were added into the cream (designated as WB), and the stirring was continued until the preparation had cooled. The placebo cream (sample P) sample did not contain wild bilberry oil or extract.