Male c57bl 6ncr mice

Male C57BL/6NCr mice were purchased from Japan SLC Inc. (Shizuoka, Japan). Ucp1^−/− (stock number 17476) was purchased from The Jackson Laboratory (Bar Harbor, ME, USA), housed under a standard 12-h light/dark cycle, and allowed ad libitum access to food and water. Mice were 9–13 weeks old at the start of testing. The experimental protocols were approved by the Animal Research Committee of Kansai Medical University.

Male C57BL/6NCr mice were purchased from Japan SLC, Inc (Shizuoka, Japan). The Trpa1^−/− mice (stock number 006401), Trpv1^−/− mice (stock number 003770), Trpa1^flox mice (stock number 008649), Omp-Cre mice (stock number 006668), RCL-GCamP6f mice (stock number 028865), and RCL-ChR2(H134R)/EYFP mice (stock number 024109) were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Trpa1-Cre⁺ mice were generated by inserting improved-Cre^{54 (link)} at the start codon of the endogenous Trpa1 locus using CRISPR/Cas9-mediated genome editing in the ES cells^{55 (link)}. RCL-ChR2(H134R)/EYFP mice were crossed with Trpa1-Cre⁺ mice to visualize Trpa1-Cre⁺ cells. The ΔD mice were generated by crossing OMACS-Cre and Eno2-STOP-DTA mice, as reported previously^{6 (link)}. Advillin-Cre mice were kindly provided by Dr. Wang^{31 (link)}. Mice were housed under a standard 12-h light/dark cycle at room temperature of 23 ± 2 °C with humidity of 30–70% and allowed ad libitum access to food and water. Mice were at least 9 weeks old at the start of testing. The experimental protocols were approved by the Animal Research Committee of Kansai Medical University.