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Active integrin β1 clone huts 4

Manufactured by Merck Group

The Active Integrin-β1 clone HUTS-4 is a laboratory reagent used for the detection and analysis of the integrin-β1 protein. It is a monoclonal antibody that specifically binds to the active conformation of the integrin-β1 subunit, allowing for the identification and quantification of active integrin-β1 in various research applications.

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2 protocols using active integrin β1 clone huts 4

1

Antibodies for Immunoblot and Immunocytochemistry

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Antibodies for immunoblotting and immunocytochemistry: GAPDH (Invitrogen, #437000), HIF1-α (Cell Signaling Technology, #14179S), HIF2-α (Abcam, #ab207607), Fibronectin (IB) (Abcam, #ab23750), Fibronectin (ICC) (SCBT, #IST-9), β-Actin (Invitrogen, #MA1–140), Vinculin (Abcam, #129002), Integrin-α6 (Millipore Sigma #MABT356), Integrin-α5 (Abcam, #ab150361), Integrin-β1 (Cell Signaling Technology, #96996) Alexa Fluor 568 (Invitrogen #A10037) and Alexa Fluor 488 (Invitrogen, #A11001). For flowcytometry, Active Integrin-β1 clone HUTS-4 (Millipore Sigma, #MAB2079Z) was used. Matrigel was purchased from Corning. Protein concentration in lots used varied between 10–11.2 mg/ml with an endotoxin level of <1.5.
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2

Antibody panel for cell analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for immunoblotting and immunocytochemistry: GAPDH (#437000; Invitrogen), HIF1‐α (#14179S; Cell Signaling Technology), HIF2‐α (#ab207607; Abcam), FN (IB) (#ab23750; Abcam), FN (ICC) (#IST‐9; SCBT), β‐actin (#MA1‐140; Invitrogen), Vinculin (#129002; Abcam), Integrin‐α6 (#MABT356; Millipore Sigma), Integrin‐α5 (#ab150361; Abcam), Integrin‐β1 (#96996; Cell Signaling Technology) Alexa Fluor 568 (#A10037; Invitrogen), and Alexa Fluor 488 (#A11001; Invitrogen). For flowcytometry, Active Integrin‐β1 clone HUTS‐4 (#MAB2079Z; Millipore Sigma) was used. Matrigel™ was purchased from Corning. Protein concentration in lots used varied between 10 and 11.2 mg/ml with an endotoxin level of less than 1.5.
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