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Raji cell

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Raji cells are a human B lymphoblast cell line derived from a Burkitt's lymphoma patient. They are suitable for a variety of cell culture and research applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) K562 cell, by American Type Culture Collection (4 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Thp 1 cell, by American Type Culture Collection (4 mentions) Hela cell, by American Type Culture Collection (4 mentions) Rpmi 1640, by Thermo Fisher Scientific (4 mentions) Glutamine, by Thermo Fisher Scientific (3 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (3 mentions) Hek293t cell, by American Type Culture Collection (3 mentions) Rpmi 1640, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Streptomycin, by Thermo Fisher Scientific (3 mentions) Hek293 cell, by American Type Culture Collection (2 mentions) Rpmi media, by Thermo Fisher Scientific (2 mentions) Iscove modified dulbecco media (imdm), by American Type Culture Collection (2 mentions) A549 and pc3 cells, by American Type Culture Collection (2 mentions) Kaign s modified f 12 k media, by Thermo Fisher Scientific (2 mentions) Peripheral blood macrophages, by STEMCELL (2 mentions) Vero e6 cell, by American Type Culture Collection (2 mentions) Expi293f cells, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Raji B cells (12 citations) Raji cell line (11 citations) Raji B lymphoblast cells (2 citations) Raji parental cells (2 citations) Raji lymphoblastoid B cell line (2 citations) Neoplastic B-lymphoid Raji cell line (2 citations) B-lymphoid Raji and Daudi cell lines (2 citations)

39 protocols using raji cell

1

EBV Virus Production and Titration

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Epstein-Barr-virus B95.8 (EBV) was produced from EBNA3A knock-out (3AKO), EBNA3C knock-out (3CKO) or wildtype (wt) BACs maintained in human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) as described elsewhere [39 (link)]. The GFP expressing virus was titrated on Raji cells (ATCC, Manassas, VA, USA) in serial dilution and the GFP-expressing cells were analyzed by flow cytometry 2 days later to calculate Raji-infectious units (RIU).
Murer A., McHugh D., Caduff N., Kalchschmidt J., Barros M., Zbinden A., Capaul R., Niedobitek G., Allday M., Chijioke O, & Münz C. (2018). EBV persistence without its EBNA3A and 3C oncogenes in vivo. PLoS Pathogens, 14(4), e1007039.
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2

Cell Culture Protocols for Various Lines

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Raji cells (ATCC) were maintained in RPMI media (Life Technologies) with 10% fetal bovine serum (FBS, Omega Scientific) and 5 mM glutamine (Life Technologies). K562 cells (ATCC) were cultured in IMDM (ATCC) media and 10% FBS. A549 and PC3 cells (ATCC) were maintained in Kaign’s modified F-12 K media (Gibco) supplemented with 10% FBS and 5 mM glutamine. Peripheral blood macrophages (Stemcell Technologies) were thawed and plated in DMEM (ATCC) and supplemented with 5 mM glutamine and 10% FBS for at least two days and no longer than 6 days before use.
Kang J.S, & Dervan P.B. (2015). A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma. Quarterly reviews of biophysics, 48(4), 453-464.
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3

EBV Strain B95-8 Production and Titration

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EBV strain B95-8 was produced from human embryonic kidney HEK293 cells containing wild-type BACs (kind gift from H. Delecluse). This GFP expressing virus was titrated on Raji cells (ATCC, Manassus, VA, USA), and the frequency of GFP-expressing cells was analyzed by flow cytometry (BD FACSCantoII) two days post-infection to calculate Raji-infectious units (RIU).
Chatterjee B., Deng Y., Holler A., Nunez N., Azzi T., Vanoaica L.D., Müller A., Zdimerova H., Antsiferova O., Zbinden A., Capaul R., Dreyer J.H., Nadal D., Becher B., Robinson M.D., Stauss H, & Münz C. (2019). CD8+ T cells retain protective functions despite sustained inhibitory receptor expression during Epstein-Barr virus infection in vivo. PLoS Pathogens, 15(5), e1007748.
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4

Cell Culture Protocols for Various Cell Lines

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Expi293F cells (Thermo Fisher, Waltham, MA, USA, Cat# A14527) were cultured in the serum-free SMM 293-TI medium (Sino Biological Inc., Beijing, China) at 37 °C with 8% CO2 on an orbital shaker platform. HEK293T cells (Cat# CRL-3216) and Vero-E6 cells (cat# CRL-1586) were acquired from ATCC and cultured in 10% Fetal Bovine Serum (FBS, GIBCO cat# 16140071) supplemented with Dulbecco’s Modified Eagle Medium (DMEM, ATCC cat# 30-2002) at 37 °C, 5% CO2. Raji cells (Cat# CCL-86) and THP-1 cells (cat# TIB-202) were acquired from ATCC and cultured in 10% FBS supplemented with Roswell Park Memorial Institute (RPMI) 1640 medium (Thermo Fisher, cat# 31870-082) at 37 °C, 5% CO2. I1 mouse hybridoma cells (ATCC, cat# CRL-2700) were cultured in Eagle’s Minimum Essential Medium (EMEM, ATCC cat# 30-2003) with 20% FBS.
Wang X., Li M., Lu P., Li C., Zhao C., Zhao X., Qiao R., Cui Y., Chen Y., Li J., Cai G, & Wang P. (2023). In Vitro Antibody-Dependent Enhancement of SARS-CoV-2 Infection Could Be Abolished by Adding Human IgG. Pathogens, 12(9), 1108.
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5

Cell Culture Conditions for Cell Lines

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PC-3/HLA-A2 and HeLa (ATCC® CCL-2) were cultured in D10 medium (high-glucose DEME supplemented with 10% fetal bovine serum, 100 U/mL Penicillin and 100 μg/mL Streptomycin. Raji cells (ATCC® CCL-86) were cultured in RPMI 1640 medium supplemented with 10% FBS and 100 U/mL Penicillin and 100 μg/mL Streptomycin. HeLa cells and Raji cells were obtained from the American Type Culture Collection (Manassas, VA). The cell lines have not been authenticated. All cells were cultured at 37°C with 5% CO2.
Zhou Y., Shao N., de Castro R.B., Zhang P., Ma Y., Liu X., Huang F., Wang R.F, & Qin L. (2020). Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip. Cell reports, 31(4), 107574.
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6

Cell Culture Conditions for Cell Lines

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PC-3/HLA-A2 and HeLa (ATCC® CCL-2) were cultured in D10 medium (high-glucose DEME supplemented with 10% fetal bovine serum, 100 U/mL Penicillin and 100 μg/mL Streptomycin. Raji cells (ATCC® CCL-86) were cultured in RPMI 1640 medium supplemented with 10% FBS and 100 U/mL Penicillin and 100 μg/mL Streptomycin. HeLa cells and Raji cells were obtained from the American Type Culture Collection (Manassas, VA). The cell lines have not been authenticated. All cells were cultured at 37°C with 5% CO2.
Zhou Y., Shao N., de Castro R.B., Zhang P., Ma Y., Liu X., Huang F., Wang R.F, & Qin L. (2020). Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip. Cell reports, 31(4), 107574.
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7

Cell culture conditions for various cell lines

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HEK293 cells (ATCC CRL-1573), HT-29 cells (ATCC HTB-38), U-251 MG cells (CVCL_0021), SH-SY5Y cells (ATCC CRL-2266), HeLa cells (ATCC CCL-2), and their KO derivatives were cultured in Dulbecco's Modified Eagle Medium (DMEM) with high glucose (Nacalai Tesque). Raji cells (ATCC CCL-86), THP-1 cells (ATCC TIB-202), K-562 cells (ATCC CCL-243), and their derivatives were cultured in RPMI medium 1640 (Nacalai Tesque). HAP1 cells (kindly provided by Thijn Brummelkamp, The Netherlands Cancer Institute, Amsterdam, The Netherlands) and their KO derivatives were cultured in Iscove's Modified Dulbecco's Medium (Nacalai Tesque). CHO (Chinese Hamster Ovary) K1 cells (ATCC CCL-61) and their derivatives were maintained in DMEM/F-12 (Nacalai Tesque). All cell culture medium contained 10% heat-inactivated fetal bovine serum (FBS) (172012-500ML, Sigma). All cells were maintained in an incubator at 37 °C and 5% CO2.
Wang Y., Menon A.K., Maki Y., Liu Y.S., Iwasaki Y., Fujita M., Guerrero P.A., Silva D.V., Seeberger P.H., Murakami Y, & Kinoshita T. (2022). Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 119(14), e2115083119.
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8

Lentiviral Vector Production and Cell Transduction

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293T cells (ATCC, Manassas, VA, USA) were transfected using Lipofectamine 3000 (ThermoFisher, Waltham, MA, USA) with a luciferase construct, human PVR lentiviral construct, or green fluorescence protein construct obtained from VectorBuilder (Chicago, IL, USA) to generate lentiviral vector and Raji cells (ATCC, Manassas, VA, USA) were transduced. Luciferase-expressing cells were selected with puromycin for two weeks. PVR-expressing cells were selected with blasticidin (10μg/mL) for two weeks. GFP-expressing cells were FACS sorted.
Jackson Z., Hong C., Schauner R., Dropulic B., Caimi P.F., de Lima M., Giraudo M.F., Gupta K., Reese J.S., Hwang T.H, & Wald D.N. (2022). Sequential single cell transcriptional and protein marker profiling reveals TIGIT as a marker of CD19 CAR-T cell dysfunction in patients with non-Hodgkin’s lymphoma. Cancer discovery, 12(8), 1886-1903.
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9

Primary Human Keratinocyte Isolation and Culture

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Primary normal human epidermal keratinocytes (named primary human KCs in the paper) from the juvenile foreskin of a single donor were purchased from Promocell. KCs were cultured in serum-free medium (keratinocyte growth medium 2 (KGM-2), Promocell, Heidelberg). In some experiments, KCs were treated overnight with 100 ng/mL IFNγ (BioLegend, San Diego, CA) (as indicated in the respective figures).
Raji cells (ATCC, Manassas, CCL-86; Burkitt’s lymphoma cell line) were used as pAPCs. Raji cells were cultured in RPMI1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA) + 10 % FBS (PAN-BioTech, Aidenbach).
PBMCs were obtained from heparinized blood from healthy donors using Ficoll-Hypaque (Linaris, Dossenheim) density-gradient centrifugation. PBTs or naive CD4+ T cells were isolated from PBMCs using a Pan-T-cell isolation kit or naive CD4+ T-cell isolation kit (Miltenyi Biotec, Bergisch Gladbach). This study was approved by the Ethics Committee of Heidelberg University (S-089/2015).
Orlik C., Deibel D., Küblbeck J., Balta E., Ganskih S., Habicht J., Niesler B., Schröder-Braunstein J., Schäkel K., Wabnitz G, & Samstag Y. (2019). Keratinocytes costimulate naive human T cells via CD2: a potential target to prevent the development of proinflammatory Th1 cells in the skin. Cellular and Molecular Immunology, 17(4), 380-394.
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10

Producing Infectious EBV Viral Particles

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Infectious viral particles were produced from 293 cell lines stably infected with the M81 virus [56 (link)] or the M81-Luc virus following transfection with EBV BZLF1 and GP110 expression vectors as previously described [62 (link)]. EBV was titered on Raji cells (ATCC) using the Green Raji cell assay as previously described [62 (link)].
Bilger A., Plowshay J., Ma S., Nawand ar D., Barlow E.A., Romero-Masters J.C., Bristol J.A., Li Z., Tsai M.H., Delecluse H.J, & Kenney S.C. (2017). Leflunomide/teriflunomide inhibit Epstein-Barr virus (EBV)-induced lymphoproliferative disease and lytic viral replication. Oncotarget, 8(27), 44266-44280.
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