Ewing sarcoma cell lines (n = 21) were obtained from multiple sources: L-1062 and L-872 were established in-house [21 (link)]; CHP100, RM-82, IARC-EW7, TC32 and 6647, CHP100, RM-82, IARC-EW-7, WE-68, IARC-EW-3, STA-ET-2.1, TTC-466, STA-ET-10, CADO-ES1, TC-71, VH-64, COH and STA-ET-1 were obtained from the EuroBoNeT consortium collection (Institute of Pathology, University Medical Center, Düsseldorf, Germany) [22 (link)] and SK-ES-1, SK-NM-C, A-673 and R-D-ES from the American Type Culture Collection (ATCC). All cell lines and primary culture L-4027 were cultured in a monolayer under equal conditions and in Iscove’s modified Dulbecco’s medium containing GlutaMAX supplement, supplemented with 1 % streptomycin/penicillin and 10 % heat-inactivated FCS (all from Life Technologies, Bleiswijk, The Netherlands). Authentication of cell lines using Powerplex 1.2 and CellID STR (Promega, Leiden, the Netherlands) and mycoplasma DNA Q-PCR screening were regularly performed on all cell lines.
Vh 64
Manufactured by American Type Culture Collection
Sourced in Germany
The VH-64 is a laboratory instrument designed for the efficient storage and retrieval of biological samples. It provides a controlled environment for the preservation of cultures, cell lines, and other biological materials.
Automatically generated - may contain errors
Lab products found in correlation
We 68, by American Type Culture Collection (2 mentions)
Powerplex 1, by Promega (1 mentions)
Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Cellid str, by Promega (1 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Rd es, by American Type Culture Collection (1 mentions)
Sk es 1, by American Type Culture Collection (1 mentions)
Sk n mc, by American Type Culture Collection (1 mentions)
Ttc 466, by American Type Culture Collection (1 mentions)
Vincristine, by Selleck Chemicals (1 mentions)
Etoposide, by Selleck Chemicals (1 mentions)
Gsk2830371, by Selleck Chemicals (1 mentions)
Doxorubicin, by Cell Signaling Technology (1 mentions)
Chla258, by American Type Culture Collection (1 mentions)
2 protocols using vh 64
1
Ewing Sarcoma Cell Lines Comprehensive Characterization
2
Cell Line Characterization and Compound Synthesis
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Cell lines were obtained from the American Type Culture Collection (ATCC), except for VH-64 and WE-68, which were provided by J. Sonnemann (Universitätsklinikum Jena, Jena, Germany); TC138 and CHLA258, which were purchased from the COG Cell Line and Xenograft Repository; and SJSA-X, which was provided by G. Wahl (The Salk Institute for Biological Studies, La Jolla, CA). Cell line identity was confirmed by Short Tandem Repeat (STR) profiling. ATSP-7041 and ATSP-7342 were synthesized according to established methods (Bird et al., 2011 (link); Chang et al., 2013 (link)). XL-188 was synthesized according to established methods (Lamberto et al., 2017 (link)). RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO.
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