Reagent grade monobasic monohydrate sodium phosphate

Manufactured by Thermo Fisher Scientific

Reagent grade monobasic monohydrate sodium phosphate is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The primary function of this product is to serve as a chemical for use in various laboratory experiments and analyses.

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Lab products found in correlation

Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (2 mentions) Ethyl acetate, by Thermo Fisher Scientific (2 mentions) Lc ms grade water, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Ketoconazole, by Merck Group (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Formic acid, by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions)

Close spelling variants of this product

Sodium phosphate monobasic (37 citations) Sodium phosphate (37 citations) Sodium phosphate monobasic monohydrate (30 citations) Reagent Grade (5 citations)

2 protocols using reagent grade monobasic monohydrate sodium phosphate

Metabolic Profiling of Drug Candidates

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Animal experiments were approved by the Animal Use and Care Committee of University of California, Davis. Male rats (Sprague Dawley, 250–300 g) were purchased from the Charles River Laboratories (CA). Liver S9 fractions from human, monkey, dog, rat, and mouse were purchased from XenoTech, LLC (Lenexa, KS). TPPU and its four metabolites as well as 1-(1-acetylpiperidin-4-yl)-3-(4-(trifluoromethyl)phenyl)urea (TAPU) and 12-(3-cyclohexyl-ureido)-dodecanoic acid (CUDA) were synthesized in house and their syntheses are described in the supplementary material. Reagents for the NADPH regeneration system, including beta-nicotinamide adenine dinucleotide phosphate sodium salt (NADP⁺), D-glucose-6-phosphate dehydrogenase (G6PDH), D-glucose-6-phosphate monosodium salt (G6P), anhydrous magnesium chloride (MgCl₂), sodium chloride (NaCl), ethylenediaminetetraacetic acid (EDTA), and glacial acetic acids were obtained from Sigma (St. Louis, MO). LC-MS grade water, methanol (MeOH), acetonitrile, ethyl acetate (EA), reagent grade monobasic monohydrate sodium phosphate, and anhydrous dibasic sodium phosphate were purchased from Fisher Scientific (Pittsburgh, PA).

Wan D., Yang J., McReynolds C.B., Barnych B., Wagner K.M., Morisseau C., Hwang S.H., Sun J., Blöcher R, & Hammock B.D. (2019). In vitro and in vivo Metabolism of a Potent Inhibitor of Soluble Epoxide Hydrolase, 1-(1-Propionylpiperidin-4-yl)-3-(4-(trifluoromethoxy)phenyl)urea. Frontiers in Pharmacology, 10, 464.

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Cytochrome P450 Enzyme Assay Protocol

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All the animal experiments were conducted following the protocols approved by the Animal Use and Care Committee of University of California-Davis. Male rats (Sprague Dawley, 10–12 weeks) and male mice (C57BL/6, 8 weeks) were purchased from the Charles River Laboratories (CA). Cytochrome P450s (CYPs), rat and human liver S9 fraction were purchased from BD Biosciences (Franklin Lakes, NJ). 1-(5-Butoxypentyl)-3-adamantylurea (surrogate), 1-adamantanyl-3-decylurea (ADU, internal standard), AEPU and t-AUCB, were synthesized in this laboratory by using the methods previously published [27 (link), 30 (link), 37 (link)]. β-Nicotinamide adenine dinucleotide phosphate sodium salt (NADP+), glucose-6-phosphate dehydrogenase (G6PDH), D-glucose-6-phosphate monosodium salt (G6P), anhydrous magnesium chloride (MgCl₂), sodium chloride (NaCl), ketoconazole, Chromium trioxide, sulfuric acid, and formic acid (98 %) were obtained from Sigma (St. Louis, MO). HPLC-grade methanol, acetonitrile, ethyl acetate, reagent-grade monobasic monohydrate sodium phosphate and anhydrous dibasic sodium phosphate were purchased from Fisher Scientific (Pittsburgh, PA). Water (>18.0 MΩ) used was purified by NANO pure II system (Barnstead, Newton, MA).

Liu J.Y., Tsai H.J., Morisseau C., Lango J., Hwang S.H., Watanabe T., Kim I.H, & Hammock B.D. (2015). In vitro and in vivo metabolism of N-adamantyl substituted urea-based soluble epoxide hydrolase inhibitors. Biochemical pharmacology, 98(4), 718-731.

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