Nis e software

Manufactured by Nikon

Sourced in Japan

NIS-E software is a digital imaging and analysis platform developed by Nikon. It provides tools for image capture, processing, and analysis across a range of microscopy applications. The software interfaces with Nikon microscopes and cameras to enable seamless image acquisition and manipulation.

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Lab products found in correlation

N sim, by Nikon (3 mentions) Ixon du 897 emccd camera, by Oxford Instruments (2 mentions) Lsm 710, by Zeiss (2 mentions) Eclipse ti e inverted research microscope, by Nikon (1 mentions) Cfi apo tirf 100 oil objective lens, by Oxford Instruments (1 mentions) Fv10i, by Olympus (1 mentions) Fluorescent labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti oga, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Biosesang (1 mentions)

4 protocols using nis e software

Visualizing Senile Plaque-Induced Capillary Changes

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To identify the modification of cerebral capillaries by senile plaque, we used the super-resolution Structured Illumination Microscope (Nikon N-SIM, Nikon). 3D-SIM images of each fixed brain slices were taken by moving the stage in the z-direction with a step size of 0.15 μm. The sequential z-sections were reconstructed to create a 3D-SIM image (z axis; brain slices thickness about 5.0±0.4 μm) and produce the 3D-deconvolution with alpha blending function using NIS-E software (Nikon). Images were taken with an Eclipse Ti-E inverted research microscope, using CFI Apo TIRF × 100 oil objective lens (NA=1.49, Nikon) and 512 × 512-pixel resolution iXon DU-897 EMCCD camera (Andor Technology, Belfast, UK). Multicolor fluorescence analysis was performed using a diode laser (488, 561 nm; exposure time, 40 ms; EM gain, 150; conversion gain, × 1), and images were processed with the NIS-E software (Nikon, Tokyo, Japan) and exported to the Adobe Photoshop program.

Kook S.Y., Lee K.M., Kim Y., Cha M.Y., Kang S., Baik S.H., Lee H., Park R, & Mook-Jung I. (2014). High-dose of vitamin C supplementation reduces amyloid plaque burden and ameliorates pathological changes in the brain of 5XFAD mice. Cell Death & Disease, 5(2), e1083-.

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Super-Resolution Microscopy Imaging Protocol

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We used super-resolution structured illumination microscopy (SIM; Nikon N-SIM). The raw images were reconstructed to three-dimensional-SIM images using NIS-E software (Nikon). Images were acquired using an Eclipse Ti-E research inverted microscope with Nikon’s legendary CFI Apo TIRF 100× oil objective lens (NA 1.49) and 512 × 512 pixel resolution equipped with an iXon DU-897 EMCCD camera (Andor Technology). Multicolor fluorescence was acquired using a diode laser (488 nm, 561 nm).

Lee K.H., Kim S.H., Kim H.J., Kim W., Lee H.R., Jung Y., Choi J.H., Hong K.Y., Jang S.K, & Kim K.T. (2014). AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation. Nucleic Acids Research, 42(6), 3590-3606.

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3D-SIM Imaging of P-gp Expression

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To clarify the modification of P-gp expression by amyloid plaques, we used a super-resolution structured illumination microscopy (SIM; Nikon N-SIM, Nikon Instruments, Inc., Tokyo, Japan) as described.^{25 (link)} Briefly, images of the immunofluorescent stained brain slices were taken sequentially in the z-direction in order to reconstruct them into 3D-SIM images by NIS-E software (Nikon Instruments, Inc.).

Park R., Kook S.Y., Park J.C, & Mook-Jung I. (2014). Aβ1–42 reduces P-glycoprotein in the blood–brain barrier through RAGE–NF-κB signaling. Cell Death & Disease, 5(6), e1299-.

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Analysis of OGA and c-Fos Interaction

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For analyzing OGA and c‐Fos interaction, we performed immunocytochemistry as previously described (Kim et al., 2012). Briefly, EGFP‐c‐Fos‐WT transfected cells were plated on coverslips and treated by Aβ. After fixed by 4% paraformaldehyde (BIOSESANG, Inc., Korea), cells were incubated with anti‐OGA (Sigma, USA) and GFP (OSE00001G, Osenses, Australia) antibodies, which were diluted in 0.5% Tx‐100, 1% goat serum in PBS, overnight at 4°C. After labeling with fluorescent‐labeled secondary antibodies (Invitrogen, USA) for 1 hr at room temperature, cells were imaged by SIM (Nikon N‐SIM, Nikon Instruments, Inc., Japan). Images were processed and analyzed by NIS‐E software (Nikon Instruments, Inc., Japan). For imaging c‐Fos level, native EGFP signals were imaged by confocal microscopy (Olympus FV10i; Olympus, Japan and LSM710; Carl Zeiss, Germany) in living cells which were transfected by EGFP‐c‐Fos‐WT and treated by CHX or MG132.

Choi H., Kim C., Song H., Cha M., Cho H.J., Son S.M., Kim H.J, & Mook‐Jung I. (2018). Amyloid β‐induced elevation of O‐GlcNAcylated c‐Fos promotes neuronal cell death. Aging Cell, 18(1), e12872.

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