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2hp β cd

Manufactured by Merck Group
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2HP-β-CD is a lab equipment product offered by Merck Group. It serves as a key component in various analytical and research applications. The product provides a specific function within the laboratory setting, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

2 hp α cd, by Merck Group (2 mentions) 2 hp γ cd, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Potassium dihydrogen phosphate, by Avantor (1 mentions) Sodium chloride (nacl), by Avantor (1 mentions) Hydrochloric acid (hcl), by Avantor (1 mentions) Dimethyl sulfoxide (dmso), by Avantor (1 mentions) Trimeb, by Merck Group (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Hionic fluor scintillation cocktail, by PerkinElmer (1 mentions) Solvable solution, by PerkinElmer (1 mentions) Sodium phosphate dibasic, by Thermo Fisher Scientific (1 mentions) Ethanol, by Avantor (1 mentions) Sodium phosphate monobasic, by Thermo Fisher Scientific (1 mentions) Carprofen rimadyl, by Zoetis (1 mentions) Hydrogen peroxide, by Thermo Fisher Scientific (1 mentions) Polyethylene glycol 300 peg300, by Merck Group (1 mentions) Heparin sodium salt, by Merck Group (1 mentions) L name, by Merck Group (1 mentions) Pentobarbital, by Merck Group (1 mentions)

12 protocols using 2hp β cd

1

Cyclodextrin-Enhanced Cefuroxime Axetil Tablets

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The uncoated tablets were prepared by mixing the weighed amount of cyclodextrin with the cefuroxime axetil (USP Reference Standard No. 1098220, Merck, Darmstadt, Germany) in the ratio of masses: 1 part of active substance + 2 parts of cyclodextrin (α-, β-, γ, and 2HP-β-CD, respectively). CDs (α-CD, CAS 10016-20-3; β-CD, CAS 7585-39-9; γ-CD CAS 17465-86-0; 2HP-β-CD, CAS 128446-35-5) were obtained from Sigma-Aldrich, Saint Louis, MO, USA. The substitution pattern was deduced from the information obtained from the manufacturer about the molar mass and the extent of labeling. The ingredients were thoroughly mixed in a mortar and then ground for 30 min to obtain a homogeneous mass. The homogeneous mass was compressed and tableted in an EK0 impact tablet press (Korsch-Erweka, Langen, Germany). Flat, triple stamps with a diameter of 3 mm were used, and the punch pressure was set to 30 kN. All procedures were performed at room temperature. The average weight of the tablet was 17 mg and hardness was 0.6 kG. For Raman microspectroscopy, the whole, uncrushed tablets were measured.
Gieroba B., Kalisz G., Sroka-Bartnicka A., Płazińska A., Płaziński W., Starek M, & Dąbrowska M. (2021). Molecular Structure of Cefuroxime Axetil Complexes with α-, β-, γ-, and 2-Hydroxypropyl-β-Cyclodextrins: Molecular Simulations and Raman Spectroscopic and Imaging Studies. International Journal of Molecular Sciences, 22(10), 5238.
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2

Solubilization of LCRF-0006 and Bortezomib

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For in vitro experiments, LCRF‐0006 (kindly provided by Crocus Laboratories) was solubilized in dimethyl sulfoxide (DMSO) (Merck). For in vivo experiments, LCRF‐0006 was solubilized in saline containing 40% 2‐hydroxypropyl‐β‐cyclodextrin (2‐HP‐β‐CD; Merck). Bortezomib (VELCADE®; Janssen‐Cilag Pty Ltd) was reconstituted at a final concentration of 0.028% DMSO for in vitro studies and 1.33% DMSO for in vivo studies.
Mrozik K.M., Cheong C.M., Hewett D.R., Noll J.E., Opperman K.S., Adwal A., Russell D.L., Blaschuk O.W., Vandyke K, & Zannettino A.C. (2020). LCRF‐0006, a small molecule mimetic of the N‐cadherin antagonist peptide ADH‐1, synergistically increases multiple myeloma response to bortezomib. FASEB BioAdvances, 2(6), 339-353.
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3

Cyclodextrin-Based Permeability Assay

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RA (purity > 95%); 2-HP-α-CD (molar substitution 0.6, Mw~1.180); 2-HP-β-CD (molar substitution 0.8, Mw~1.460); and 2-HP-γ-CD (0.5–0.7 molar substitution, Mw~1.580) were purchased from Sigma-Aldrich (Poznan, Poland). Acetonitrile (high-performance liquid chromatography [HPLC] grade) was provided by Merck (Darmstadt, Germany), and other chemical reagents—including hydrochloric acid, dimethyl sulfoxide, sodium chloride, and potassium dihydrogen phosphate—were obtained from Avantor Performance Materials (Gliwice, Poland). Formic acid 98–100% was purchased from POCH (Gliwice, Poland). Prisma HT, GIT lipid solution, and acceptor sink buffer were supplied by Pion Inc (Forest Row, East Sussex, England).
Stasiłowicz-Krzemień A., Rosiak N., Płazińska A., Płaziński W., Miklaszewski A., Tykarska E, & Cielecka-Piontek J. (2022). Cyclodextrin Derivatives as Promising Solubilizers to Enhance the Biological Activity of Rosmarinic Acid. Pharmaceutics, 14(10), 2098.
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4

Licoricidin Injection for DIO Mice

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Licoricidin was dissolved in PBS containing 2-hydroxypropyl β-cyclodextrin (2-Hp-β-CD, Sigma-Aldrich, USA). The DIO mice were anesthetized with isoflurane and then licoricidin (10 μg/kg) was injected daily into the bilateral inguinal fat pads using a microsyringe (Smiths Medical ASD, Inc., Keene, NH, USA), according to previously described 30 . The same volume of PBS containing 2-Hp-β-CD was injected daily into vehicle mice in the control group without licoricidin. The weight of each mouse was monitored daily.
Jin Z., Meng W., Xiao T., Deng J., Wang J., Wen J., Chen K., Wang L., Liu J., Li Q., He J., Wang Z., Liu W, & Liu F. (2023). Vertical sleeve gastrectomy-derived gut metabolite licoricidin activates beige fat thermogenesis to combat obesity. Theranostics, 13(9), 3103-3116.
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5

Cyclodextrin-Enabled Thymidine Derivative Study

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2-HP-α-CD, 2-HP-β-CD, 2-HP-γ-CD, DIMEB and TRIMEB CDs were purchased from Sigma-Aldrich (Milan, Italy) and used without further purification. The same commercial source was adopted for D2O and LiClO4 (99.99% trace metals basis). 4-Thio(2’-deoxy)thymidine (S4TdR), having 99+% purity was purchased from Carbosynth (Compton, Berkshire, UK). S4TdR and CDs stock solutions (at concentrations of 10−2 M and 5 × 10−2 M, respectively) were prepared in doubly distilled water or, in D2O for 1H NMR analysis and in the presence of LiClO4 (0.1 M) for CV experiments. The solutions were stored at 4 °C.
Rizzi V., Matera S., Semeraro P., Fini P, & Cosma P. (2016). Interactions between 4-thiothymidine and water-soluble cyclodextrins: Evidence for supramolecular structures in aqueous solutions. Beilstein Journal of Organic Chemistry, 12, 549-563.
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6

NPC1 Mice Treated with 2HPβCD

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The NPC1 mice (Npc1nih/nih) we used originated from a colony at the National Institutes of Health. Control (Npc1+/+) and homozygous (Npc1−/−) mice were generated from heterozygous (Npc1+/−) breeding stock on a pure BALB/c background. Pups were weaned and genotyped at 19 to 21 days of age. After weaning, all mice were fed a basal low-cholesterol rodent chow diet ad libitum as described.29 (link) The mouse room had alternating 12-h periods of dark and light. Npc1−/− and Npc1+/+ mice that had not received any prior treatments were administered weekly a subcutaneous injection at the scruff of the neck of either normal saline or a 20% (w/v) solution (in saline) of 2HPβCD (Sigma-Aldrich Corp; product H107) starting when they were 49-days old. This provided an approximate dose of 4000 mg/kg bw.12 (link) As described below, the treatment period varied according to the types of measurements to be made. Mice were studied in the fed state in all experiments except the one involving bile collection when they were fasted for 4 h beforehand. All experimental protocols were approved by the Institutional Animal Care and Use Committee of The University of Texas Southwestern Medical Center.
Lopez A.M., Terpack S.J., Posey K.S., Liu B., Ramirez C.M, & Turley S.D. (2014). Systemic administration of 2-hydroxypropyl-β-cyclodextrin to symptomatic Npc1-deficient mice slows cholesterol sequestration in the major organs and improves liver function. Clinical and experimental pharmacology & physiology, 41(10), 780-787.
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7

Modulating Cholesterol Homeostasis in Zebrafish

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The stock solutions were prepared by dissolving the powdered compound in Milli-Q water to a final concentration of 100 mM for (2-hydroxypropyl)-β-cyclodextrin (2HPβCD; Sigma, catalog no. C0926) and 10 mM for 3-β-[2-(diethylamino)ethoxy]androst-5-en-17-one (U18666A; Sigma, catalog no. 662015). Good-quality eggs were placed in 9 cm diameter Petri dishes filled with 25 ml of E3 medium and kept under static condition at 28 ± 0.5°C. At 3 dpf, the medium was changed to fresh E3 medium supplemented with U18666A (8 μM), 2HPβCD (2 mM), and U18666A (8 μM) together with 2HPβCD (2 mM). As a control, an equal volume of the solvent was used. Embryos were kept in static condition for 2 days. At 5 dpf, the fish were stained with Nile blue and collected for RNA extraction.
Wiweger M., Majewski L., Adamek-Urbanska D., Wasilewska I, & Kuznicki J. (2021). npc2-Deficient Zebrafish Reproduce Neurological and Inflammatory Symptoms of Niemann-Pick Type C Disease. Frontiers in Cellular Neuroscience, 15, 647860.
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8

Synthesis and Characterization of Radiolabeled E2

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Carprofen (Rimadyl) was purchased from Zoetis (Parsippany, NJ, USA). Tritiated E2, dissolved in ethanol, was purchased from American Radiolabeled Chemicals (St. Louis, MO, USA). Solvable Solution and Hionic-Fluor scintillation cocktail were purchased from PerkinElmer (Waltham, MA, USA). Randomly methylated β-CD, 2HP-β-CD, β-CD, γ-CD, polyethylene glycol 300 (PEG300), heparin sodium salt, and non-radioactive E2 were purchased from Sigma-Aldrich (St. Louis, MO, USA), and ethanol was purchased from VWR (Radnor, PA, USA). Non-toxic black paint was purchased from Blick (Highland Park, IL, USA). Additional chemicals and solutions, including sodium chloride, 30% hydrogen peroxide, monobasic sodium phosphate, and dibasic sodium phosphate, were purchased from Fisher Scientific (Waltham, MA, USA).
Prakapenka A.V., Peña V.L., Strouse I., Northup-Smith S., Schrier A., Ahmed K., Bimonte-Nelson H.A, & Sirianni R.W. (2020). Intranasal 17β-Estradiol Modulates Spatial Learning and Memory in a Rat Model of Surgical Menopause. Pharmaceutics, 12(12), 1225.
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9

Preclinical Evaluation of IGF1R/IRS Inhibitor NT157

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NT157 (HY-100037 Medchemexpress LLC), a selective inhibitor targeting the IGF1R/IRS signaling pathway, was used for in vivo pre-clinical studies. NT157 was dissolved in 20% 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD: H-107 Sigma) at 5 mg/ml. NT157 (70 mg/kg) or vehicle was injected three times per week through intraperitoneal route. For mouse tumor models, 4T1 or E0771 cells (3 × 105) were injected into #2 MFP of female Balb/c or C57Bl/6 mice respectively. For 4T1 orthotopic mammary tumor model, the treatment was given starting on day 10 after tumor inoculation. For the E0771 metastasis model, E0771 cells were injected in the MFP and followed by 1 × 105 cells through the tail vein on day 12. After NT157 treatment for 3 weeks, tumor weight, spleen weight, and the number of lung metastases were assessed. Gr-1+CD11b+ myeloid cells were isolated from spleens of treated mice and used for various assays.
Ishii H., Vodnala S.K., Achyut B.R., So J.Y., Hollander M.C., Greten T.F., Lal A, & Yang L. (2018). miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity. Nature Communications, 9, 2611.
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10

Cellular Assay with Biological Compounds

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2HP-β-CD, pentobarbital, L-NAME and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, USA). Crystal violet was purchased from Biotime (Jiangsu, P.R.China).
Qi X., Yuan Y., Xu K., Zhong H., Zhang Z., Zhai H., Guan G, & Yu G. (2015). (2-Hydroxypropyl)-β-Cyclodextrin Is a New Angiogenic Molecule for Therapeutic Angiogenesis. PLoS ONE, 10(5), e0125323.
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