Rolera em c2

Manufactured by Zeiss

The Rolera EM-C2 is a high-performance EMCCD camera designed for scientific imaging applications. It features a back-illuminated sensor with a large field of view and high quantum efficiency, providing excellent low-light sensitivity and fast frame rates.

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Lab products found in correlation

Axiovert 200m, by Zeiss (2 mentions) Axio imager, by Zeiss (2 mentions) Axio observer, by Zeiss (1 mentions) Axioskop, by Zeiss (1 mentions)

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Rolera Em-C2 camera (3 citations)

2 protocols using rolera em c2

Fluorescence Microscopy of Immobilized Nematodes

Cited 1 time

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Live animals were mounted on 10% agarose pads and immobilized using 0.1um polystyrene microspheres (Polysciences 00876–15) [42 (link)]. Multiwavelength fluorescence Z-series with 0.2 micron step size were obtained using a SOLA SE 365 Solid state light engine, Axiovert 200M (Carl Zeiss MicroImaging) microscope equipped with a digital CCD camera (QImaging; Rolera EM-C2), or Axio Observer (Carl Zeiss MicroImaging) microscope equipped with a scientific sCMOS camera (Photometrics Prime 95B), captured using MetaMorph 7.7 software (Universal Imaging) and then deconvolved using AutoDeblur X3 software (AutoQuant Imaging). Colocalization analysis was done using MetaMorph 7.7 colocalization plugin, whereby intensities in each channel were thresholded and Pearson’s correlation was analyzed. Intensity measurements were also analyzed by MetaMorph 7.7, using thresholded images captured under identical acquisition parameters. FRAP was done as described in [4 (link)].

Norris A., McManus C.T., Wang S., Ying R, & Grant B.D. (2022). Mutagenesis and structural modeling implicate RME-8 IWN domains as conformational control points. PLOS Genetics, 18(10), e1010296.

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Fluorescence Microscopy Analysis of Worm Genetics

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Worms were visualized using Zeiss Axioskop and AxioImager microscopes equipped for DIC and fluorescence microscopy. Images were captured using Zeiss Axiocam MRm or QImaging Rolera em-c² cameras using Zeiss Zen software. To count ceh-22∷gfp positive nuclei, Z-series images were collected. Maximum intensity Z projections were produced using Fiji/ImageJ (Schindelin et al., 2012 (link)). To quantify TBX-2∷GFP fluorescence, Z-series of L1 animals were collected using identical exposure times that avoided saturating image intensity. Mean and maximum GFP fluorescence intensity was measured using Fiji/ImageJ in 33–42 head hypodermal nuclei in appropriate image planes from at least 9 animals. Results are reported for mean fluorescence intensity. Maximal fluorescence intensity measurements produced very similar results.

Huber P., Crum T, & Okkema P.G. (2016). Function of the C. elegans T-box factor TBX-2 depends on interaction with the UNC-37/Groucho corepressor. Developmental biology, 416(1), 266-276.

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