Cellbind culture dishes

Manufactured by Corning

Sourced in United States

CellBind culture dishes are a line of laboratory equipment designed for cell culture applications. The dishes provide a specialized surface to support the growth and maintenance of cells in vitro. The product features are focused on the core function of facilitating cell attachment and proliferation without any interpretation or extrapolation on intended use.

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Lab products found in correlation

Ix71 microscope, by Olympus (4 mentions) Accutase, by Innovative Cell Technologies (2 mentions) Neurocult ns a differentiation kit, by STEMCELL (1 mentions) Neurocult xf basal medium, by STEMCELL (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Fibroblast growth factor 2 fgf2, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CellBIND (52 citations) CellBind dishes (11 citations) CellBIND tissue culture dishes (3 citations) CellBIND cell culture dishes (2 citations)

9 protocols using cellbind culture dishes

Primary Sphere Propagation from drNPCs

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Monolayers of drNPCs were lifted off from adherent Corning® CellBIND® culture dishes (Corning, Product #2394 to #3296) using Accutase (Innovative Cell Technologies, Inc. Catalog #AT-104), and the resulting cell suspension was plated on Corning™ Costar™ 24-well Ultra-Low Attachment Surface Plates (Fisher Scientific, Product #07-200-602) at a 10 cells/μL density in the same medium used for culturing monolayers. After a 1 week incubation period, single primary spheres were dissociated into single cells and replated in fresh medium. Dissociation of cells consisted of suspending spheres in Accutase for 3 min at 37 °C and mechanically triturating the solution 20 times. The number of secondary spheres were counted in each well 1 week later.

Ahlfors J.E., Azimi A., El-Ayoubi R., Velumian A., Vonderwalde I., Boscher C., Mihai O., Mani S., Samoilova M., Khazaei M., Fehlings M.G, & Morshead C.M. (2019). Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells. Stem Cell Research & Therapy, 10, 166.

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Neural Precursor Cell Differentiation

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Monolayers of drNPCs were plated onto adherent Corning® CellBIND® culture dishes (Corning, Product #2394 to #3296) in either maintenance culture medium (as mentioned above) or in the NeuroCult NS-A Differentiation Kit, comprising of NeuroCult XF basal medium (catalog # 05760) and NeuroCult™ NS-A Differentiation Supplement (Human) (Component# 0574) (StemCell Technologies). Media was changed after every 2–3 days until each plate was used for PCR analysis. The differentiation commenced for 10 days, and the differentiation profile was analyzed.

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Isolation and Characterization of Rhesus Macaque Monocyte-Derived Macrophages

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Peripheral blood was obtained in heparinized vacutainer collection tubes from healthy rhesus macaques of Chinese origin at the Center for Animal Experiment (Wuhan University School of Medicine) under animal care procedures according to Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC). Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of rhesus macaques by Ficoll gradient centrifugation. Macaque PBMCs were plated in 48-well-plate (3×10⁶cells/well) or 96-well-plate (0.8×10⁶cells/well) Cell BIND culture dishes (Corning, USA) with RPMI1640 containing 2% autologous serum, 100 U/ml penicillin (Gibco), 100 μg/ml streptomycin (Gibco) and 1% non-essential amino acids (Gibco) for 24h. After the removal of non-adherent cells from the cultures, the monocytes were cultured with RPMI1640 containing 2% autologous serum for 7 days when monocytes differentiated into macrophages. Macaque monocytes cultured with RPMI1640 containing 2% autologous serum for 4 days (Fig. 1A) or 7 days (Fig. 1B) showed typical macrophage morphology, which was confirmed by CD14 staining with purity of day 4 (Fig. 1C) and day 7 (Fig. 1D) were 91.3 % and 96.5 %, respectively. Viability of the cells cultured for 4 and 7 days exceed 96.3 % and 95.8 % respectively. Poly I:C treatment had little effect on cell viability (supplemental Fig. 1).

Sang M., Liu J.B., Dai M., Wu J.G, & Ho W.Z. (2014). Toll-Like Receptor 3 Signaling Inhibits Simian Immunodeficiency Virus Replication in Macrophages from Rhesus Macaques. Antiviral research, 0, 103-112.

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Quantifying Cell Cluster Area

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Cells were grown to confluency on CellBind Culture Dishes (Corning, NY, USA). Cell monolayers were rinsed twice with DMEM and then cells were detached in DMEM using a cell scraper. Detached cells were dissociated by pipetting ten times with a 1 mL pipet tip. Images were obtained of 25–30 fields per dish using a 10× objective from a IX71 Olympus microscope. Areas of cell clusters with more than 10 cells were measured using Image J Software.

Hall M.K., Shajahan A., Burch A.P., Hatchett C.J., Azadi P, & Schwalbe R.A. (2023). Limited N-Glycan Processing Impacts Chaperone Expression Patterns, Cell Growth and Cell Invasiveness in Neuroblastoma. Biology, 12(2), 293.

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Embryonic Fibroblast Wound Healing Assay

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WT and CaD-deficient E14.5 ± 0.5 embryonic fibroblasts were seeded in equal concentrations of 2 × 10⁵/3 ml onto 35-mm CellBind culture dishes (Corning) using Dulbecco’s modified Eagle Medium (DMEM)-complete (DMEM high glucose, 10% FBS, 2 mM L-glutamine, 100 units/ml penicillin-streptomycin, and 1× nonessential amino acids). After ∼48 h, the cells were confluent, so the medium was removed completely, and cell wounds were made in the monolayer using a premium surface 200-µl pipet tip (Nerbe Plus). Cells were rinsed twice with DMEM, 3 ml each, and overlaid with 3 ml DMEM-complete medium. Wound closure was documented by JuLi Br Live cell movie analyzer (Peqlab) over a time frame of 24 h, using a P4x plus digital zoom objective and a high-resolution digital camera containing a 5-megapixel CMOS-color sensor, resulting in images of 2,560 × 1,920 px. For analysis with ImageJ software, 25 images were acquired on an hourly basis, and the distance between the cell boundaries was determined (in pixels) at four previously set positions.

Pütz S., Barthel L.S., Frohn M., Metzler D., Barham M., Pryymachuk G., Trunschke O., Lubomirov L.T., Hescheler J., Chalovich J.M., Neiss W.F., Koch M., Schroeter M.M, & Pfitzer G. (2021). Caldesmon ablation in mice causes umbilical herniation and alters contractility of fetal urinary bladder smooth muscle. The Journal of General Physiology, 153(7), e202012776.

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Cell Aggregate Measurement Protocol

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Cells were seeded on 35 mm CellBind culture dishes (Corning, NY, USA) and allowed to grow to confluence for 2 days [21 (link)]. In short, cells were rinsed twice with media and re-suspended in serum free media. Cells were detached by one complete rotation with a cell scraper. Detached cells were dissociated by pipetting ten times with a 1 mL pipet tip. Images (25–30 fields/dish) were acquired on an Olympus IX 71 microscope using a 10X objective. Area of cell aggregates (≥ 10 cells/aggregate) were measured using Image J software.

Hall M.K., Weidner D.A., Whitman A.A, & Schwalbe R.A. (2018). Lack of complex type N-glycans lessens aberrant neuronal properties. PLoS ONE, 13(6), e0199202.

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Culturing Human Neural Progenitor Cells

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Human cells were cultured as a monolayer on Corning® CellBIND® culture dishes (Corning, Product #2394 to #3296) in low oxygen conditions in 5% CO₂; 5% O₂, and 37 °C. Complete Human NeuroCult XF medium (StemCell Technologies) was supplemented with epidermal growth factor (EGF) [20 ng/ml] (Peprotech), fibroblast growth factor-2 (FGF-2) [30 ng/ml] (Peprotech), and heparin [100 μg/ml] (Scientific Protein Laboratories). Accutase (Innovative Cell Technologies, Inc. Catalog #AT-104) was used for detaching the cells. Cells were passaged 1:4 or 1:8 upon reaching < 80% confluency. Cells were fed by replacing 50% of the medium every 36 h.

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Cell Dissociation Assay Protocol

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Cells were grown to confluency on 35 mm CellBind culture dishes (Corning, Corning, NY, USA), as described [13 (link)]. HuNB(-MGAT2) cells transiently transfected for 24 h were used for the cell dissociation assay. Cells were resuspended in serum-free media following two rinses with media, and then cells were detached by one complete rotation with a cell scraper. Using a 1 mL pipet tip, detached cells were dissociated by pipetting 14 times. An Olympus IX 71 microscope using a 20 × objective was used to acquire images (25–30 fields/dish). Image J software was employed to measure the area of cell aggregates (≥10 cells/aggregate).

Hall M.K., Whitman A.A., Weidner D.A, & Schwalbe R.A. (2020). Knockdown of N-Acetylglucosaminyltransferase-II Reduces Matrix Metalloproteinase 2 Activity and Suppresses Tumorigenicity in Neuroblastoma Cell Line. Biology, 9(4), 71.

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Cell Aggregate Analysis Protocol

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Cells were plated at similar concentrations and grown for 2 days on 35 mm CellBind culture dishes (Corning, Corning, NY, USA) [7 (link)]. At confluency, cells were washed twice and then resuspended in media. Cells were detached with a cell scraper, and then cell aggregates were dissociated by pipetting fifteen times. Images (30–35 fields/dish) were acquired on an Olympus IX 71 microscope with a 10× objective. Particles (≥5 cells/aggregate) were counted, and their areas determined by employment of Image J software. Data is given as the mean ± S.E. and n denotes the number of particles. Statistical comparison was evaluated via student’s t-test. Statistical significance was considered at p < 0.05.

Hall M.K., Weidner D.A., Zhu Y., Dayal S., Whitman A.A, & Schwalbe R.A. (2016). Predominant Expression of Hybrid N-Glycans Has Distinct Cellular Roles Relative to Complex and Oligomannose N-Glycans. International Journal of Molecular Sciences, 17(6), 925.

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