After the final DTT measurement the samples were removed from the heating block and allowed to cool for 5 minutes. An aliquot of each solution was filtered through a 0.22 μM PTFE syringe filter (Tisch Environmental) and diluted 1:10 with 3% nitric acid to adequately dilute the salts prior to analysis. Filter blanks and daily solution blanks were treated in the same way as PM samples. Soluble metals were analyzed by the UC Davis Interdisciplinary Center for Plasma Mass Spectrometry (ICPMS.UCDavis.edu) using an Agilent 7500CE ICP-MS (Agilent Technologies, Palo Alto, CA). Standards were diluted from a CertiPrep ME2A standard (SPEX CertiPrep) to 0.25 ppb, 0.5 ppb, 1 ppb, 10 ppb, 100 ppb, 200 ppb and 500 ppb respectively in 3% nitric acid. A NIST 1643E Standard metals calibration (National Institute of Standards and Technology) was analyzed initially and QC standard consisting of ME2A at 100 ppb were analyzed every 12th sample as quality controls. Sc, Y, and Bi CertiPrep standards (SPEX CertiPrep) were diluted to 100 ppb in 3% nitric acid and introduced by peripump as an internal standard.
Certiprep
Manufactured by SPEX
Sourced in United States
CertiPrep is a laboratory equipment product designed for sample preparation. It is used to ensure consistent and accurate sample preparation for various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
Agilent 7500ce icp ms, by Agilent Technologies (1 mentions)
Dneasy kit, by Qiagen (1 mentions)
Precellys cryolys, by Bertin Technologies (1 mentions)
X series 2, by Thermo Fisher Scientific (1 mentions)
Optima 5300, by PerkinElmer (1 mentions)
Trace metal grade, by Thermo Fisher Scientific (1 mentions)
Emx spectrometer, by Bruker (1 mentions)
7900 icp ms instrument, by Agilent Technologies (1 mentions)
Vortex mixer, by Thermo Fisher Scientific (1 mentions)
Digiprep jr, by SCP Science (1 mentions)
Surine negative urine control, by Cerilliant (1 mentions)
Optima 4300 dv, by PerkinElmer (1 mentions)
Icap qc, by Thermo Fisher Scientific (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Ft ir 4100, by Jasco (1 mentions)
N ethylmaleimide, by Merck Group (1 mentions)
Benzamidine, by Merck Group (1 mentions)
Iodoacetic acid, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
32 protocols using certiprep
1
Soluble Metals Analysis by ICP-MS
2
Quantitative Copper Analysis via GFAAS
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Cu was quantitated with a graphite furnace atomic absorption spectrometer (PerkinElmer AAnalystTM 600), with copper standards (SPEX CertiPrep®) diluted in 2% trace element grade nitric acid.
3
Anticoagulant Rodenticide Residue Analysis
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Residue levels of both brodifacoum and diphacinone were determined in liver and whole-body remainder (the entire carcasses without the liver and tissues removed for histopathology, as well as turtle carcasses with shells removed) by HPLC. Specifically, livers were homogenized using liquid nitrogen with a mortar and a pestle. The carcasses were skinned and processed through a meat grinder followed by liquid nitrogen milling (SPEX Certiprep). Samples of 500 mg of each homogenate were weighed into Teflon microwave tubes and then heated to 51.7 °C. Each sample was analyzed twice: once for diphacinone residues and again for brodifacoum residues. Chlorophacinone and difenacoum were added to each sample to serve as surrogate standards for diphacinone and brodifacoum, respectively. Diphacinone and brodifacoum concentrations were determined using an Agilent 1100 series HPLC system and Gemini C18, 3.0 μm, 150 × 3.0 mm column (Phenomenex). Brodifacoum and difenacoum were detected using fluorescence with an excitation wavelength of 273 nm and an emission wavelength of 390 nm.
4
Cartilage Extraction and DNA Isolation
5
Serum Zinc Concentration Determination
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Participants were asked to provide overnight fasting blood samples. tube was used and serum zinc concentration was determined by inductively coupled plasmamass spectrometry (ICP-MS) using a PerkinElmer mass spectrometer (PerkinElmer, Waltham, MA, USA). Serum samples were diluted with two per cent nitric acid, and serum zinc concentration was obtained from a linear relationship (r = 0.999) between concentrations of zinc stock standard (1,000 mg/ml, SPEX CertiPrep, Metuchen, NJ, USA) and absorbance. The accuracy of the analytical procedures was verified with standard reference material (ClinChek Serum Controls, lyophilised for trace elements, RECIPE, Munich, Germany). The standard deviation index was 0.50, and coefficients of variation for inter-and intra-assay were two per cent, and four per cent, respectively. 30
6
Liver Extraction and Preservation
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All rodents were necropsied to remove the liver for analysis. An incision was made in the skin covering the abdomen and the skin was pulled back. A lateral incision was then made at the base of the breastbone and a pair of scissors used to cut the breastbone on each side. The liver was removed from each rodent and ground to a fine powder using a liquid nitrogen freezer mill (SPEX CertiPrep, Metuchen, NJ, USA) and stored in individual vacuum sealed bags at -30°C until analysis.
7
Quantifying AuNR Uptake in NSCs
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NSCs were incubated with AuNRs (5 × 1012 AuNRs/mL) for 16 h. AuNR-loaded NSCs were carefully washed with PBS buffer (1×) three times to remove unloaded AuNR. A 1 mL amount of BDH Aristar Plus Nitric Acid (70%) was used to digest the cell pellet, and the sample was diluted to 5 mL with 2% HNO3. Gold concentration was determined by ICP-MS analysis on a Thermo X-Series 2 using a concentric nebulizer, impact bead spray chamber, and torch with a fixed quartz injector. An Xt interface was used. Plasma power was 1250 W, nebulizer flow was 0.82 L/min, cool gas flow was 14 L/min, and auxiliary gas flow was 1.1 L/min. Sample flow to the nebulizer was approximately 400 μL/min. A standard curve was made using serial dilutions of a 1 ppm solution of gold standard solution (Spex CertiPrep). Data were analyzed quantitatively in a spreadsheet program.
8
Platinum-Based Cancer Therapy Protocol
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PPI (Lansoprazole; Astra-Zeneca, Mölndal, Sweden) was resuspended in DMSO immediately before use. In combination treatment experiments, cells were pretreated for 24 hours with PPI and then treated for additional 6 hours with 2 µM Cisplatin (Teva Italia, Milan, Italy).
For the separation of the chemical forms of CisPt the following reagents were used: trifluoromethanesulfonic acid (triflic) (Sigma-Aldrich), methanol of chromatography grade (Lab Scan, Analytical Sciences, Dublin, Ireland), sodium dodecyl sulphate (SDS, Scientific Supplies, Auckland, NZ), sterile 0.9% saline solution. Other chemicals were of analytical grade unless otherwise indicated. To analysis CisPt present in cells, exosomes, cell culture medium and tumour tissues the elemental Pt content was detected, using a monoelemental Pt standard solution (Spex CertiPrep, Metuchen, NJ, USA).
For the separation of the chemical forms of CisPt the following reagents were used: trifluoromethanesulfonic acid (triflic) (Sigma-Aldrich), methanol of chromatography grade (Lab Scan, Analytical Sciences, Dublin, Ireland), sodium dodecyl sulphate (SDS, Scientific Supplies, Auckland, NZ), sterile 0.9% saline solution. Other chemicals were of analytical grade unless otherwise indicated. To analysis CisPt present in cells, exosomes, cell culture medium and tumour tissues the elemental Pt content was detected, using a monoelemental Pt standard solution (Spex CertiPrep, Metuchen, NJ, USA).
9
Soil Edaphic Factors and Climate Analysis
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Several edaphic factors were analyzed in bulk soil samples: soil temperature, pH, moisture, total carbon, and total nitrogen. Soil temperature was collected at sampling by insertion of a temperature probe into the open holes after soil coring. Soil pH was measured in a soil and water suspension (2:5 soil/water). Moisture content was obtained by the oven-drying method (at 105°C). Subsamples of each soil were pulverized in a ball mill (Spex CertiPrep, Metuchen, NJ) prior to total C and N measurement by dry combustion (900°C) with an Elementar vario MAX cube elemental analyzer (Hanau, Germany).
Suffolk region monthly precipitation and air temperature measurements for 2011 and 2012 were obtained from annual climatological summaries of Mattituck Station (40.990°N, 72.512°W) extracted from the National Oceanic and Atmospheric Administration (NOAA) Web page (http://www.ncdc.noaa.gov/cdo-web/datasets/ANNUAL/stations/COOP:305142/detail ).
Suffolk region monthly precipitation and air temperature measurements for 2011 and 2012 were obtained from annual climatological summaries of Mattituck Station (40.990°N, 72.512°W) extracted from the National Oceanic and Atmospheric Administration (NOAA) Web page (
10
Acid Digestion for Environmental Elemental Analysis
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