5975 gas chromatograph

Gas chromatographic investigation was performed on a 5975 Gas Chromatograph coupled with mass spectrometer (Agilent, USA; SEM Ltd., Istanbul, Turkey). As a stationary phase, Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) was used, while helium was the mobile phase (0.8 mL/min). The volume injected was 0.1 µL with a split ratio of 40:1. The oven temperature of the GC was at first fixed at 60 °C for 10 min, then increased to 220 °C at a rate of 4 °C/min, held constant for 10 min and thereafter increased to 240 °C at a rate of 1 °C/min. The injector and the transfer line temperatures were fixed at 250 and 280 °C, respectively. MS detection was carried out at 70 eV with scan mass range m/z 35–450.

Kumquat peel EO composition was determined using gas chromatography coupled with mass spectrometry (GC–MS) (7890A/5975C, Agilent, USA). An Agilent computerized system comprising a 5,975 gas chromatograph coupled with a 7890A mass spectrometer was used. Gas chromatography analyses were performed with the HP 5,975 gas chromatograph equipped with a FID detector and an HP‐5™ fused silica capillary column (30 m × 0.25 μm × 0.25 μm film thickness) using helium as the carrier gas (1.0 ml/min) at a splitting ratio of 1:20. The injector and detector temperature were 280°C and 250°C, respectively. The oven temperature was initially held at 60°C for 2 min, then linearly increased by 8°C/min until reaching 250°C, and then held for 20 min. Ionization was obtained by electronic impact under a potential of 70 eV, with an ion source temperature of 230°C and the quadrupole temperature of 150°C. The mass spectra were recorded on a selective quadrupolar type Hewlett‐Packard detector model 7890A. Identification of components was mainly based on the comparison of their GC Kovats retention indices (RI), determined with reference to an homologous series of C8–C40 n‐alkanes. GC retention times were also analyzed, and computer matching with the NIST 11 library and comparison of the fragmentation patterns with those reported in the research literature were also performed to ensure accuracy.

Gas chromatographic analysis was performed on a 5975 Gas Chromatograph coupled with-mass spectrometer (Agilent, USA; SEM Ltd., Istanbul, Turkey). Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) was utilized as stationary phase while helium was utilized as a moble phase (0.8 mL/min). The volume injected was 0.1 μL with a split ratio of 40:1. The oven temperature of the GC was intialy set at 60 °C for 10 min, then increased to 220 °C at a rate of 4 °C/min, held constant for 10 min and thereafter increased to 240 °C at a rate of 1 °C/min. The temperatures of the injector and transfer line were set at 250 and 280 °C respectevely. MS detection was performed at 70 eV with scan mass range m/z 35–450.