Axsym analyzer

Manufactured by Abbott

Sourced in United States, Germany

The AxSYM analyzer is a compact, automated immunoassay analyzer designed for clinical diagnostic laboratories. It utilizes fluorescence polarization immunoassay (FPIA) technology to perform a variety of immunoassay tests, including those for therapeutic drug monitoring, endocrinology, and infectious disease detection. The AxSYM analyzer can process a wide range of sample types, including serum, plasma, and urine, and offers high throughput capabilities to meet the demands of busy clinical laboratories.

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Lab products found in correlation

Axsym, by Abbott (1 mentions) Digoxin 3 reagent pack, by Abbott (1 mentions) Immulite 1000, by Siemens (1 mentions) Cobas integra analyser, by Roche (1 mentions) Ax sym hcv 3, by Abbott (1 mentions) Monolisa anti hbc plus, by Bio-Rad (1 mentions) Axsym hbsag v2, by Abbott (1 mentions)

Close spelling variants of this product

AxSYM (135 citations) AxSYM immunoassay analyzer (3 citations) AxSYM automatic biochemical analyzer (2 citations)

13 protocols using axsym analyzer

Homocysteine Quantification in NHANES

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For NHANES 2001, total Hcy in plasma was quantified by a fully automated fluorescence polarization immunoassay (FPIA) (Abbott Hcy IMX (HCY) assay, Abbott Laboratories, Chicago, IL, USA) [23 ]. For NHANES 2002 and 2003–2004, total Hcy in plasma was measured by other FPIA (Abbott Diagnostics (Abbott Axsym analyzer, Abbott^®), Abbott Laboratories, Chicago, IL, USA) [23 ,24 (link),25 ]. Both methods employed the same reagent kit. Moreover, both approaches presented equivalence as the FPIA results were successfully compared to high performance liquid chromatography with fluorometric detection at 385 nm excitation and 515 nm emission [23 ,25 ]. Hyperhomocysteinemia was defined as total Hcy in plasma >15 µmol/L [4 (link),5 (link)].
In both FPIA approaches [23 ,24 (link),25 ], dithiothreitol (DTT) was used to free thiol, then S-adenosyl-Hcy (SAH) hydrolase was applied to catalyze the conversion of Hcy to SAH in the presence of added adenosine. Then, FPIA was performed using a specific monoclonal antibody and fluoresceinated SAH analog tracer [24 (link)]. In the first FPIA method, total Hcy concentrations were calculated by the Abbott IMx^® (Illinois, USA) using a machine-stored calibration curve, while in the second was calculated by the Abbott Axsym^® (Illinois, USA) using a machine-stored calibration curve [26 (link),27 (link)].

Botelho J., Machado V., Leira Y., Proença L, & Mendes J.J. (2021). Periodontal Inflamed Surface Area Mediates the Link between Homocysteine and Blood Pressure. Biomolecules, 11(6), 875.

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Homocysteine Measurement via FPIA

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For NHANES 2003–2006, homocysteine levels in plasma were assessed using fluorescence polarization immunoassay (FPIA; Abbott Diagnostics (Abbott AxSym analyzer, Abbott^®), Chicago, IL, United States). In FPIA method, dithiothreitol (DTT) was employed for thiol liberation, followed by the application of S-adenosyl-homocysteine (SAH) hydrolase to catalyze homocysteine conversion to SAH in the presence of added adenosine. Subsequently, FPIA was executed utilizing a specific monoclonal antibody and a fluoresceinated SAH analog tracer. Total homocysteine concentrations in the FPIA method were computed using the Abbott AxSym^® machine, utilizing a pre-stored calibration curve (18 (link)).

Wu P., Ma J., Yang S., Wu H., Ma X., Chen D., Jia S, & Yan N. (2024). Association between homocysteine and blood pressure in the NHANES 2003–2006: the mediating role of Vitamin C. Frontiers in Nutrition, 11, 1379096.

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Serum Iron Biomarkers in HUNT2 Cohort

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Non-fasting serum samples were drawn in 1995–1997 (HUNT2). Serum iron concentration (µmol L⁻¹) was determined using a FerroZine method using a Hitachi 911 Autoanalyzer (reagents from Boehringer, Germany). The serum transferrin concentration (µmol L⁻¹) was analyzed by an immunoturbidimetric method using the Hitachi 911 Autoanalyzer (reagents from DAKO A/S, Denmark), and calculated for a molecular weight of 79,570 Da. TIBC was calculated as 2 × the serum transferrin concentration. The TSP was calculated as 100 × [serum iron concentration/TIBC]. Serum ferritin (µg L⁻¹) was measured from serum samples using an Abbott AxSYM analyzer (reagents from Abbott Laboratories, USA). In total, 56,667 HUNT participants had measurements of serum iron and TIBC, 56,664 had measurements of TSP, while ferritin was only measured in 2334 women (fertile, non-pregnant, aged 20–55 years).

Moksnes M.R., Graham S.E., Wu K.H., Hansen A.F., Gagliano Taliun S.A., Zhou W., Thorstensen K., Fritsche L.G., Gill D., Mason A., Cucca F., Schlessinger D., Abecasis G.R., Burgess S., Åsvold B.O., Nielsen J.B., Hveem K., Willer C.J, & Brumpton B.M. (2022). Genome-wide meta-analysis of iron status biomarkers and the effect of iron on all-cause mortality in HUNT. Communications Biology, 5, 591.

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Digoxin Assay Sensitivity Determination

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SDC was measured on the AxSYM analyzer (Abbott Laboratories, Abbott Park, IL) using the Digoxin III Reagent pack (Ref. 6L07, rev. September 2010, Abbott, Wiesbaden, Germany). The sensitivity of the AxSYM Digoxin II assay was calculated to be 0.3 ng/mL.

Pastori D., Carnevale R., Nocella C., Bartimoccia S., Novo M., Cammisotto V., Piconese S., Santulli M., Vasaturo F., Violi F, & Pignatelli P. (2018). Digoxin and Platelet Activation in Patients With Atrial Fibrillation: In Vivo and In Vitro Study. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(22), e009509.

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Comprehensive Metabolic Profile Assessment

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Alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT) and HDL-cholesterol (HDL-C) were measured on a Cobas MODULAR analyzer (Roche, Basel, Switzerland). Triglycerides (TG) were measured on a Hitachi 912 analyzer (Roche, Basel, Switzerland). Blood glucose was measured from venous whole blood samples using an EPOS Analyzer 5060 (Eppendorf, Hamburg, Germany). Insulin was determined by microparticle enzyme immunoassay (MEIA) on an AXSYM analyzer (Abbot, Abbot Park, USA), C-peptide (CP) was measured chemiluminimetrically (Immulite1000, Siemens, Erlangen, Germany).

Kahl S., Straßburger K., Nowotny B., Livingstone R., Klüppelholz B., Keßel K., Hwang J.H., Giani G., Hoffmann B., Pacini G., Gastaldelli A, & Roden M. (2014). Comparison of Liver Fat Indices for the Diagnosis of Hepatic Steatosis and Insulin Resistance. PLoS ONE, 9(4), e94059.

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Measuring Ammonia and Valproic Acid Levels

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Blood samples were taken during the interictal state. As transient hyperammonemia has been reported to be associated with seizures,^{17 (link)} the blood samples collected from the patients with a recent seizure event were excluded from this study. In our clinical practice, blood samples are collected into a heparinized tube that is then immediately placed in ice water. Blood samples were centrifuged at 3000 rpm for 10 minutes to obtain serum samples. The plasma ammonia and VPA levels were rapidly analyzed by the central laboratory of Chang Gung Memorial Hospital. In brief, the level of ammonia was measured by a timed endpoint method using an ammonia reagent kit (Bechman Coulter, Brea, CA). The changes in the absorbance at 340 nm were monitored by a UniCel DxC 880i system (Bechman Coulter) to calculate the concentration of ammonia. A fluorescence polarization immunoassay was used to measure the level of VPA in conjunction with an AxSYM analyzer (Abbott Laboratories, Abbott Park, IL). The blood samples were sent to the central laboratory for the analysis of complete blood cell count and serum levels of creatinine, alanine aminotransferase, alkaline phosphatase, and gamma glutamyl transpeptidase (γGT). The cut-off value of the reference level of ammonia is 93 µg/dL. Therefore, we defined hyperammonemia as patients whose blood ammonia level was higher than 93 µg/dL.

Tseng Y.L., Huang C.R., Lin C.H., Lu Y.T., Lu C.H., Chen N.C., Chang C.C., Chang W.N, & Chuang Y.C. (2014). Risk Factors of Hyperammonemia in Patients With Epilepsy Under Valproic Acid Therapy. Medicine, 93(11), e66.

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Measurement of Serum Iron Biomarkers

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Blood samples were collected from patients in fasting states. From each patient, 5–8 mL of whole blood was drawn into vacutainers and centrifuged at 1000× g for 20 min at 25 °C to separate blood cells and serum. After collection, serum samples were aliquoted and stored at −80 °C until further analysis. Serum iron and transferrin (Cobas Integra analyser from Roche, Basel, Switzerland), and serum ferritin (AxSym analyzer from Abbott, Chicago, IL, USA) were immediately determined. A colorimetric (Ab932715, AbCam, Cambridge, UK) and an enzyme-linked immunosorbent assay (Ab288174, AbCam, Cambridge, UK) were used to measure total iron-binding capacity (TIBC) following manufacturer instruction. Briefly, Human Anti-Transferrin antibodies were used to measure the amount of transferrin in the sample. In parallel, iron standard solutions were added to serum samples to saturate the transferrin. The amount of iron remaining free was then measured by colorimetry at a neutral pH using deferoxamine mesylate as iron chelator. Total iron was then measured after acidification by HCl. All measurements were used to calculate total iron binding capacity of the biological samples.

Schiano E., Cappello E., Cecere D., Pompeo F., Novellino E., Stornaiuolo M, & Izzo M. (2023). Increased Levels of Circulating Iron-Albumin Complexes in Peripheral Arterial Disease Patients. Antioxidants, 12(2), 503.

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Comprehensive Metabolic Biomarker Profiling

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A multichannel analyzer and dedicated kits (Humastar®, HUMAN Diagnostics, Wiesbaden, Germany) were used for the colorimetric, enzymatic measurement of cholesterol (kit: monotest cholesterol with cholesterol esterase, cholesterol oxidase and peroxidase), triglycerides (kit: peridochrom triglyceride with glycerol phosphate oxidase and peroxidase) and glucose (kit: glucose, glucose oxidase and peroxidase). Plasma LDL-cholesterol levels were calculated according to the Friedewald equation. High-density lipoprotein cholesterol levels were measured after sodium phosphotungstate/magnesium chloride precipitation of chylomicrons and VLDL and LDL-cholesterol and then centrifugation. Plasma insulin levels were measured using a microparticle enzyme immune assay running on an AxSYM analyzer (Abbott Laboratories, Abbott Park, Illinois, USA).

Boulenouar H., Hetraf S.A., Djellouli H.O., Meroufel D.N., Fodil F.Z., Hammani-Medjaoui I., Mehtar N.S., Houti L, & Benchekor S.M. (2020). Lack of association between genetic variants in the 19q13.32 region and CHD risk in the Algerian population: a population-based nested case-control study. African Health Sciences, 20(2), 735-744.

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Acute Hepatitis A Infection Screening

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To detect acute hepatitis A infection, serum samples from pediatric patients diagnosed with hepatitis were screened for the presence of anti-HAV IgM and the absence of anti-HAV IgG. All samples were negative for antibodies to HBV, HCV, and HEV. The presence of anti-HAV IgM and the absence of anti-HAV IgG, the surface antigen of HBV (HBsAg), and anti-HCV antibodies were tested by using a third-generation microparticle immunoenzymatic assay (AxSYM HAVAB-M 2_0, AxSYM HBsAg (V2), and AxSYM HCV 3.0; Abbott Laboratories, Chicago, IL) with an AxSYM analyzer (Abbott Laboratories). Total anti-hepatitis B core antigen anti-HBc (total IgM and IgG) and anti-HEV antibodies were measured by using immunoenzymatic assays (Monolisa Anti-HBc PLUS, Bio-Rad Laboratories, Chicago, IL, MP Diagnostics, Geneva, Switzerland and MyBiosource, San Diego, CA, USA, resp.) with a PR 3100 TSC analyzer (Bio-Rad). The levels of albumin/globulin, ALT, AST, alkaline phosphatase, total protein, total BR, and CB were measured in the serum samples, following routine clinical laboratory procedures.

Corral-Jara K.F., Trujillo-Ochoa J.L., Realpe M., Panduro A., Gómez-Leyva J.F., Rosenstein Y., Jose-Abrego A., Roman S, & Fierro N.A. (2016). Conjugated Bilirubin Differentially Regulates CD4+ T Effector Cells and T Regulatory Cell Function through Outside-In and Inside-Out Mechanisms: The Effects of HAV Cell Surface Receptor and Intracellular Signaling. Mediators of Inflammation, 2016, 1759027.

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Serological Screening of Blood Donors

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Serologic testing was performed by the microparticle enzyme immunoassay HBsAg (V2) on an AxSYM analyzer (Abbott, TX, USA) and anti-HBc chemiluminescence immunoassay on a Vitros ECIQ analyzer (Ortho Clinical Diagnostics, NJ, USA).
For statistical calculations, the number of VBDs tested in the Osijek-Baranja County that were rejected on the basis of serum markers was compared with the total number of VBDs tested in the Republic of Croatia; the latter data were kindly provided by the Croatian Institute of Transfusion Medicine, Zagreb.

Samardžija M., Drenjančević D., Miletić M., Slavulj B., Jukić I., Zibar L., Mihaljević S., Ferenac Kiš M, & Samardžija M. (2020). THE IMPACT OF POSITIVE ANTI-HBC MARKER ON PERMANENT DEFERRAL OF VOLUNTARY BLOOD DONORS IN EASTERN CROATIA AND ESTIMATION OF OCCULT HEPATITIS B VIRUS INFECTION RATE. Acta Clinica Croatica, 59(1), 126-134.

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