Common reagents were from Sigma-Aldrich-Merck (St Louis, MO, USA) or Roche (Darmstadt, DE). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Abcam (Cambridge, UK) or Cell Signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, ES).
Serum and culture media
Manufactured by Thermo Fisher Scientific
Sourced in United States
Serum and culture media are laboratory products used to support the growth and maintenance of cells in culture. They provide essential nutrients, growth factors, and other components necessary for cell survival and proliferation. These products are commonly used in cell biology research, tissue engineering, and cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Tissue culture dishes, by BD (6 mentions)
Murine or human cytokines, by Thermo Fisher Scientific (3 mentions)
Taurocholic acid tca, by Merck Group (1 mentions)
Ml171, by Bio-Techne (1 mentions)
Chenodeoxycholic acid cdca, by Merck Group (1 mentions)
Specific sirna, by Horizon Discovery (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Common reagents, by Merck Group (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
7 protocols using serum and culture media
1
Diverse Reagent Sources for Experimental Protocols
2
Inhibition of NOX1 in Cell Cultures
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Common reagents were obtained from Roche (Darmstadt, Germany) or Sigma-Aldrich (St Louis, MO, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA) and serum and culture media were from Invitrogen and Gibco (Life Technologies/Thermo Fisher, Madrid, Spain). Taurocholic acid (TCA) and chenodeoxycholic acid (CDCA) were purchased from Sigma-Aldrich. The selective NADPH oxidase 1 (NOX1) inhibitor ML171 was purchased from Tocris (Biogen Científica, Madrid, Spain). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA) and Sigma-Aldrich.
3
Cytokine and Antibody Reagent Protocol
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Common reagents were from Sigma-Aldrich-Merck (Madrid, Spain) or Roche (Darmstadt, Germany). Murine and human cytokines were obtained from PeproTech (London, UK) or Merck. Antibodies were from Abcam (Cambridge, UK) or Cell signaling (Danvers, MA, USA). Specific siRNAs and a nonspecific scrambled scRNA control were from Dharmacon (Merck). Reagents for electrophoresis were from Bio-Rad (Madrid, Spain). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, Spain).
4
Cytokine and Antibody Protocol
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Common reagents were from Sigma-Aldrich-Merck (St Louis, MO, USA) or Roche (Darmstadt, Germany). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Abcam (Cambridge, UK) or Cell signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, Spain).
5
Immunoblotting and Cell Culture Protocols
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Common reagents including z-VAD.FMK were from Sigma-Aldrich (St Louis, MO, USA) or Roche (Darmstadt, Germany). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Santa Cruz Biotech (Santa Cruz, CA, USA) or Cell Signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA) and Sigma-Aldrich. Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen and Gibco (Life Technologies/Thermo Fisher, Madrid, Spain).
6
Cytokine Assay Protocol Optimization
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Common reagents were from Sigma-Aldrich-Merck (Madrid, Spain) or Roche (Darmstadt, Germany). Human cytokines were from PeproTech (London, UK) or Merck. Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, Spain).
7
Murine Cytokine Assay Protocol
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Reagents were from Sigma-Aldrich-Merck (St Louis, MO, USA) or Roche (Darmstadt, Germany). Murine cytokines and TNFα, IL6, and PGE2 ELISA kits were obtained from PeproTech (London, UK) and Cayman Chem. (Ann Arbor, MI). Antibodies were from Abcam (Cambridge, UK) or Cell Signaling (Danvers, MA, USA). Dacarbazine (DTIC) was from TEVA (Petaj Tikva, IL). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (ThermoFisher, Madrid, Spain).
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