The levels of CD13, cAMP, IL-6, and IL-8 were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Boster Bio, CA, USA), a cyclic AMP selective enzyme immunoassay (EIA) kit (Cayman Chemicals, MI, USA), a solid phase chemiluminescent ELISA kit (QuantinGlo®; ELISA, R&D Systems, MN, USA), and an IL-8 EIA kit (Invitrogen, CA, USA). The levels of IFN-β, IFN-λ1, or IFN-γ were measured using a Human IFN-β Quantikine ELISA Kit, a Human IL-29/IFN-λ1 DuoSet ELISA kit (R&D Systems), and a Quantikine Human IFN-γ Immunoassay kit.
The presence of translocated p50 and p65 in nuclear extracts was assessed using a TransAM NFκB Family Kit (Active Motif), as previously described [15 ].
The presence of translocated p50 and p65 in nuclear extracts was assessed using a TransAM NFκB Family Kit (Active Motif), as previously described [15 ].