Cobas8000 e502

The past medical history, clinical manifestations, comorbidities, treatment strategies, radiologic assessments and laboratory testing on admission were extracted from the electronic medical records. Disease severity was assessed by the OS, as indicated above. Diagnosis of Covid-19 was considered as suspected in patients with typical lung CT-scan lesions and negative SARS-CoV-2 PCR.
Laboratory variables were tested with conventional methods, including routine blood tests: blood count, renal function, inflammatory markers. Determination of CRP was performed using a Cobas8000/e502^® analyzer (Roche Diagnostic, Meylan, France) using the immunoturbidimetric method with reagents from Roche (total CV imprecision results in the laboratory = 3%). Renin and aldosterone were determined on an IDS-iSYS multi-discipline automated system (ImmunoDiagnosticSystem, Boldon, United Kingdom) using kits from IDS (total CV imprecision results in the laboratory 5% and 6% for renin and aldosterone respectively). A cutoff of 102.5 pmol/L was the lower limit of plasma aldosterone detection in our condition. Plasma cortisol and adrenocorticotropic hormone (ACTH) levels were measured by automated electrochemiluminescence assays (Cobas 8000, Roche, Basel, Switzerland). Laboratory confirmation of SARS-CoV-2 infection was determined by reverse transcription-PCR from nasopharyngeal swab specimens.

Venous blood was collected in dry and EDTA tubes and was immediately centrifuged (the samples are transported in a mean total delay of less than 3 hours (all inclusive until frozen); in the biochemistry lab 95% are treated in less than an hour and a half and 50% in less than one hour) and frozen (-80°C) until tested four years later.
From dry tube, the NT-proBNP and hs-cTnT levels were determined using an immuno-electrochemiluminescence assay on the Cobas8000/e602® immunochemistry system (Roche Diagnostics, Meylan, France). Determination of CRP was run on the Cobas8000/e502® analyzer (Roche Diagnostic, Meylan, France) using immunoturbidimetric method.
We used 1 aliquot of the EDTA plasma aliquot for the determination of ST2 (this was the first thawing.), and all patients samples were measured in 1 batch 4 years after the recruitment period. ST2 plasma concentrations were measured with a high sensitivity sandwich monoclonal immunoassay (Presage© ST2 assay, Critical Diagnostics, San Diego, California distributed in France by Eurobio society).
All other biochemistry parameters as urea, creatinine, sodium were performed on Cobas 8000/c701® and ISE (Roche, Meylan, France).