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8 ohdg

Manufactured by Cusabio
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Sourced in China

8-OHdG is a product that measures the levels of 8-hydroxy-2'-deoxyguanosine, a biomarker for oxidative stress and DNA damage. It is a laboratory assay used for research purposes.

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Lab products found in correlation

Cleaved caspase 3, by R&D Systems (1 mentions) Cytochrome c, by R&D Systems (1 mentions) Phospho nf κb, by Cell Signaling Technology (1 mentions) Nf κb, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Human 8-OHdG ELISA kit (3 citations) Rat 8-OHdG ELISA Kit (3 citations)

3 protocols using 8 ohdg

1

Cerebellum Oxidative Stress and Apoptosis

Cited 3 times
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Using commercially available ELISA kits, we measured in cerebellum homogenates the levels of 8-OHdG (Cusabio Biotech Co., Ltd., Wuhan, China), cytochrome C (R&D Systems, Minneapolis, MN, USA), cleaved caspase-3 (R&D Systems, Minneapolis, MN, USA), NF-κB and phospho-NF-κB (Cell Signaling Technology, Danvers, MA, USA) as described previously [43 (link),44 (link),45 (link)].
Hamadi N., Beegam S., Zaaba N.E., Elzaki O., Altamimi M.A, & Nemmar A. (2023). Neuroinflammation, Oxidative Stress, Apoptosis, Microgliosis and Astrogliosis in the Cerebellum of Mice Chronically Exposed to Waterpipe Smoke. Biomedicines, 11(4), 1104.
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2

Bacterial Infection and DNA Oxidation

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The strain EHEC O157:H7 EDL933 was cultured in LB medium, ΔespF and ΔespF/pespF strains were cultured in LB medium containing kanamycin at 37°C for 12 h, and HT-29/Caco2 cells were seeded in 10-cm2 culture dishes. When cells reached 95% confluence, the cells were infected with bacteria at an MOI of 100:1 and incubated at 37°C and 5% CO2. Celluar 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-OHdG) was determined using a commercial competitive ELISA kit (CUSABIO, Wuhan, China). The concentrations were normalized to a standard sample and expressed in ng/mL.
Fu M., Liang S., Wu J., Hua Y., Chen H., Zhang Z., Liu J., Li X., Zhang B., Zhao W, & Wan C. (2021). An Escherichia coli Effector Protein EspF May Induce Host DNA Damage via Interaction With SMC1. Frontiers in Microbiology, 12, 682064.
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3

Quantifying Oxidative DNA Damage

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The detection of 8-OHdG (Cusabio, China) was performed as previously described [20 (link)]. DNA from NP cells was extracted using a DNA Extractor WB kit. The levels of 8-OHdG were detected using an ELISA assay kit according to the manufacturer's protocol.
Lin H., Peng Y., Li J., Wang Z., Chen S., Qing X., Pu F., Lei M, & Shao Z. (2021). Reactive Oxygen Species Regulate Endoplasmic Reticulum Stress and ER-Mitochondrial Ca2+ Crosstalk to Promote Programmed Necrosis of Rat Nucleus Pulposus Cells under Compression. Oxidative Medicine and Cellular Longevity, 2021, 8810698.
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