Spectrospin dpx 400mhz

Melting point determination was done using open capillary tubes on an electrical melting point apparatus. Bruker spectrospin DPX-400MHz was used to record the ¹H NMR and ¹³C NMR spectra. Chemical shift (δ) values were stated in parts per million (ppm) using internal standard tetramethylsilane. The D₂O exchange confirmed the exchangeable protons (OH and NH). LC–MS/MS (PerkinElmer) was used to record the mass spectra, presented as m/z. Elemental analyses were achieved by using PerkinElmer 240 analyzer. The purity of synthesized compounds as well as progress of reaction were assessed by ascending thin layer chromatography (TLC) (silica gel G) by using methanol/chloroform (9:1 v/v) and methylene chloride/chloroform (4:1 v/v) combination as solvent system.

All reactions were performed, excluding moisture, dried solvents, and uncorrected melting points. The IR spectra were recorded as potassium bromide pallets on an Aldrich FT-IR spectrometer (Central Laboratory at Faculty of Science, Benha, Ain Shams, and Cairo Universities). Mass spectra were recorded on GCMS (gas chromatography–mass spectrometer) Shimadzu QP- 2010 Plus (Microanalytical center, Ain shams University). Elemental analysis was determined using UV light on the Ain Shams University elementary analysis system. The Bruker Spectro spin DPX-400MHz was used to record the ¹H NMR (500 MHz, DMSO-d₆) and ¹³C NMR (125 MHz, Chloroform-d) spectra. Chemical shift (d) values were stated in parts per million (ppm) using internal standard tetramethylsilane. The D₂O exchange confirmed that the exchangeable protons (OH and NH) and some other labial hydrogens are exchangeable. LC–MS/MS (PerkinElmer) was used to record the mass spectra, presented as m/z. Elemental analysis was achieved using the PerkinElmer 240 analyzer. The purity of synthesized compounds and the progress of the reaction were evaluated by ascending thin layer chromatography (TLC) (silica gel Fluka, 706, 43-50 EA) using methanol/chloroform (9:1 v/v) and methylene chloride/chloroform (4:1 v/v) as a solvent system.