The CD measurements were implemented with a MOS-500 CD spectrometer (Bio-Logic, Grenoble, France), with a 1-mm light path cell, at room temperature. The CD spectra were recorded using a 2-mm bandwidth in the far-UV region (190–250 nm). The protein concentration was 0.1 mg/ml in 0.15 M phosphate buffer, pH 6.5. The UV–VIS spectra were obtained in the 200–700 nm region at room temperature using a DU 730 UV spectrophotometer (Beckman Coulter, Brea, CA, USA), and the protein concentration was 0.5 mg/ml in 0.15 M phosphate buffer, pH 6.5.
Du 730 uv spectrophotometer
Manufactured by Beckman Coulter
Sourced in United States
The DU 730 UV spectrophotometer is a laboratory instrument designed for the measurement of light absorption in the ultraviolet and visible light regions of the electromagnetic spectrum. It is capable of quantifying the concentration of dissolved substances in liquid samples by analyzing the absorption of light at specific wavelengths.
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Lab products found in correlation
2 protocols using du 730 uv spectrophotometer
1
Protein Characterization by CD and UV-VIS
2
Spectroscopic Characterization of Dye-Decolorizing Peroxidases
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Far-UV CD spectra measurements were implemented with a MOS-500 CD spectrometer (Bio-Logic, Grenoble, France), with a 1-mm light path cell. The protein concentration was 0.1 mg mL−1 in 0.15 M phosphate buffer at pH 6.5. The CD spectra were recorded using a 2-mm bandwidth in the far-UV region (190–250 nm) at room temperature.
UV–Vis spectra were obtained in the 200–700 nm region at room temperature using a DU 730 UV spectrophotometer (Beckman Coulter, Brea, CA, USA); the Il-DyP4 concentration was 0.58 mg mL−1 in 0.1 M phosphate buffer at pH 6.0 and that of Il-MnP6 was 1.26 mg mL−1 in 0.1 M sodium acetate buffer at pH 5.9.
UV–Vis spectra were obtained in the 200–700 nm region at room temperature using a DU 730 UV spectrophotometer (Beckman Coulter, Brea, CA, USA); the Il-DyP4 concentration was 0.58 mg mL−1 in 0.1 M phosphate buffer at pH 6.0 and that of Il-MnP6 was 1.26 mg mL−1 in 0.1 M sodium acetate buffer at pH 5.9.
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