Anti ter 119 percp cy5.5 ter 119

Anti-CD31 PE-Cy7 (390), anti-CD45 PerCP-Cy5.5 (30-F11), anti-Gp38 PE (eBio8.1.1), and anti-Ter-119 PerCP-Cy5.5 (TER-119) were purchased from eBioscience. Anti-CD3 PE-Cy7 (145-2C11), anti-CD19 APC-Cy7 (6D5), anti-IgD APC (11.26c.2a), anti-CD326 PerCP-Cy5.5 (G8.8), anti-CD11a PerCP-Cy5.5, (H155-78), and anti-GFP (FM264G) were purchased from Biolegend. Anti-mouse Parm1 (EPR10009) was purchased from Abcam. Donkey anti-Rat IgG Dylight488, donkey anti-goat IgG Dylight488, and donkey anti-Rabbit IgG Alexa488 were purchased from Jackson ImmunoResearch Laboratories, Inc. HECA-452, MECA-79, MECA-367, MECA-99 were produced in our lab from hybridomas and labeled using DyLight Antibody Labeling Kit (Thermo Fisher Scientific) with fluorophores indicated. Goat F(ab)2 anti-human IgG PE, Carboxyfluorescein succinimidyl ester (CFSE) and Celltracker Violet were purchased from Invitrogen. Collagenase P and Dispase II, neutral protease, grade II were purchased from Roche. DNase I from bovine pancreas was purchased from Sigma. FITC labeled Sambucus Nigra (SNA) lectin was purchased from Vector laboratories. Polyclonal goat anti-mouse Nrp1 and mouse CD22-Fc fusion proteins were purchased from R&D systems. All reagents were titered or used according to the manufacturers’ recommendations. Antibodies used for immunoprecipitation and immunoblotting are described below.