Emx1 cre mice

Heterozygous Brpf1^l mice have been described [55 (link)]. A promoterless LacZ cassette is between two FRT sites, while two loxP sites flank exons 4–6 of Brpf1 (S1A Fig). For genotyping, genomic PCR with primers Brpf1-F1 and-R1 generated a 227-bp band for wild-type, whereas genomic PCR with primers Brpf1-F1 and-mR1 produced a 162-bp fragment for the knock-in allele (S1A Fig). The LacZ cassette was deleted by breeding with PGK1-FLPo mice (Jackson Laboratory) to obtain the conditional allele Brpf1^f (S1A Fig). Cross of Brpf1^f/+ mice with Emx1-Cre mice (Jackson Laboratory) resulted in the heterozygote Brpf1^f/+;Emx1-Cre (or Brpf1^+/-, S1A Fig), and subsequent intercrosses yielded the homozygote Brpf1^f/f;Emx1-Cre (or Brpf1^-/-, referred to as bKO). The lines were maintained on the C57BL/6J background. Heterozygous Moz^l/+ mice have been described [55 (link)] and Moz^f/+ mice were generated as described above. Cross of Moz^f/+ mice with Emx1-Cre mice (Jackson Laboratory) resulted in Moz^f/+;Emx1-Cre, and subsequent intercrosses yielded Moz^f/f;Emx1-Cre. Moz lines were maintained on a mixed C57BL/6J-CD1 genetic background. Other experimental procedures are presented in S1 Text.

Brpf1^fl/fl mice were obtained from the European Conditional Mouse Mutagenesis Program (EUCOMM, project 40402). Emx1-Cre mice were purchased from The Jackson Laboratory (Stock: 005628). A dorsal telencephalon-specific disruption of Brpf1 was accomplished using Emx1-Cre, which drives recombination in both progenitor and projection neurons in the dorsal telencephalon. Through Emx1-Cre mediated recombination, the loxP-flanked region spanning exons 4–6 in Brpf1 locus was deleted. Primer pairs 5′-TGTGCCCTGTAGAGTGTTGC-3′ and 5′-GCCTTGAGTGGCACAACATA-3′ which amplify a 227-bp band for wild-type and a 440-bp band for Brpf1^fl/fl were used. The Emx1-cre; Brpf1^fl/+ mice were referred to as Brpf1 heterozygous mice (HT), and the Emx1-cre; Brpf1^fl/fl mice were referred to as Brpf1 conditional knock-out mice (cKO). The Brpf1^fl/fl mice were referred to as wild-type mice (WT). Mice used for behavioral testing were maintained on a C57BL/6 background, and for histological analysis, mice were maintained on an ICR background. The day of vaginal plug detection was considered embryonic day 0.5 (E0.5), and the day of birth was considered postnatal day 0 (P0). All animals were bred in the animal facility at Southeast University. No obvious differences were detected between the sexes. All experiments were performed according to the approved guidelines of Southeast University.

The experiments described within this manuscript were approved by and performed following the guidelines established by the Institutional Animal Care and Use Committee (IACUC) at the Temple University School of Medicine. Mice were kept on a 12-h light: 12-h dark cycle and provided food and water ad libitum. Animals were kept at ambient temperatures between 70 and 74°C and humidity from 30% to 70%. For every experiment, 3–11 animals were analyzed. Both male and female mice were used in these studies.
BubR1^H/H mice (Baker et al., 2004 (link)) were bred with Emx1-Cre mice (Jackson Lab #005628) to generate BubR1 conditional knockout (cKO) animals (Gorski et al., 2002 (link)). BubR1 cKO animals were crossed with Trp53^fl/fl animals (The National Cancer Institute Mouse Repository # 01XC2) to generate BubR1;Trp53 double conditional knockout (dcKO) mice. Genotyping was undertaken as previously described using the primers below (Armstrong et al., 1995 (link); Jonkers et al., 2001 (link); Baker et al., 2004 (link)).
Cre- (F: GCA TTA CCG GTC GAT GCA ACG AGT GAT GAG, R: GAG TGA ACG AAC CTG GTC GAA ATC AGT GCG).
BubR1- (F: GTA AGT CTA TTT CTC CTG GAT TAA GTA G, R: CAT CTG TGT ACC ATA CGT GTG TCT GG, R: ATA TTG CTG AAG AGC TTG GCG GCG).
Trp53- (F: CAC AAA AAC AGG TTA AAC CCA G, R: AGC ACA TAG GAG GCA GAG AC).