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Cd5 percp cy5

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The CD5-PerCP-CY5.5 is a fluorescently-labeled monoclonal antibody designed for flow cytometric analysis. It is a conjugate of the CD5 antibody and the PerCP-Cy5.5 fluorophore, which emits light in the yellow-orange spectrum when excited. The core function of this product is to facilitate the identification and enumeration of CD5-positive cells in biological samples.

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Lab products found in correlation

Cd19 pe cy7, by BD (1 mentions) Cxcr5 percp cy5, by BioLegend (1 mentions) Cd4 apc vio770, by Miltenyi Biotec (1 mentions) Cd45ra pe vio770, by Miltenyi Biotec (1 mentions) Ccr6 apc, by Miltenyi Biotec (1 mentions) Cxcr3 viobright fitc cxcr5 percp cy5, by Miltenyi Biotec (1 mentions) Ccr4 pe, by Miltenyi Biotec (1 mentions) Cd127 alexa fluor 647, by BioLegend (1 mentions) Cd8 horizon450, by BioLegend (1 mentions) Cd27 bv510, by BD (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Cd62l apc, by BioLegend (1 mentions) Cd45.2 pe, by BioLegend (1 mentions) Cxcr5 biotin, by BioLegend (1 mentions) Pd 1 fitc, by BioLegend (1 mentions) Slam pe cy7, by BioLegend (1 mentions) Cd95 pe cy7, by BioLegend (1 mentions) Gapdh clone 6c5, by Santa Cruz Biotechnology (1 mentions) Gl7 fitc, by BioLegend (1 mentions) Tcrβ pacific blue, by BioLegend (1 mentions)

Close spelling variants of this product

PerCP-Cy5.5 (120 citations) CD5-PerCP (2 citations) Anti-CD5 PerCP/Cy5.5 (2 citations)

3 protocols using cd5 percp cy5

1

Multicolor Flow Cytometry Analysis of Lymphocyte Subsets

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Multicolour flow cytometric analysis was performed using the following fluorochrome-labelled anti-human monoclonal antibodies for measuring lymphocyte Th and Tfh subsets (CD4-APC-Vio770, CD45RA-PE-Vio770, CCR6-APC, CXCR3-VioBright FITC CXCR5 PerCP-Cy5.5, CCR4-PE) all from Miltenyi biotech. Tregs and Tfr were detected using BD Human Regulatory T Cell Cocktail (BD, San Jose, CA, USA), including CD4-FITC (clone SK3), CD25-PECy7 (clone 2A3), CD127 Alexa Fluor® 647 and adding CXCR5 PerCP-Cy5 and CD8 horizon450 (the last two from Biolegend). Bregs were detected using the following fluorochrome-labelled anti-human monoclonal antibodies: CD38-FITC CD1d-PE, CD19-PE-Cy7, CD5-PerCP-CY5.5, CD24-APC-H7 and CD27- BV510, all from BD Biosciences (San Jose, CA, USA).
Bergantini L., d’Alessandro M., Cameli P., Pianigiani T., Fanetti M., Sestini P, & Bargagli E. (2021). Follicular T Helper and Breg Cell Balance in Severe Allergic Asthma Before and After Omalizumab Therapy. Molecular Diagnosis & Therapy, 25(5), 593-605.
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2

Comprehensive Immune Cell Profiling

Cited 1 time
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The following antibodies were used for staining: CD4 BUV395, CD8α BUV737 (BD); CD5 PerCp-Cy5.5, CD25 PE or APC, CD44 FITC or Pacific Blue, CD45.2 PE, CD62L APC, CD69 APC, TCRβ Pacific Blue, Vα2 PE or Pacific Blue, CXCR5-biotin, PD-1 FITC, SLAM PE-Cy7, CD95 PE-Cy7, GL-7 FITC (Biolegend); B220 efluore780, CD8α efluore780, ZAP70 Alexa488, Strepavidin-A647 (Life Technology); GP66 tetramer PE or APC (NIH Tetramer Core Facility). The following antibodies were used for immunoblot analysis: ZAP70 (clone 1E7.2) (36 (link)); ZAP70-pTyr319 (clone 65E4), LAT-pTyr191, Erk1/2, Erk1/2-pThr202/pTyr204, and PLCγ-pTyr183, all from Cell Signaling Technology; anti-phosphotyrosine (4G10), PLCγ (Sigma-Aldrich), LAT (clone FL-233, Santa Cruz Biotechnology), FLAG (clone M2, Sigma-Aldrich), and GAPDH (clone 6C5, Santa Cruz Biotechnology).
Shen L., Matloubian M., Kadlecek T.A, & Weiss A. (2021). A disease-associated mutation that weakens ZAP70 autoinhibition enhances responses to weak and self ligands. Science signaling, 14(668), eabc4479.
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3

Multiparametric Immune Cell Analysis

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Single immune cell suspensions were prepared from spleen or peritoneal lavage after red blood cell lysis. Cells were counted on a BD Fortessa Flow cytometer using ‘Fluoresbrite TM Calibration Grade 6.0 micron YG microspheres (Polysciences). Cells were incubated with anti-CD16/CD32 (Fc block, clone 2.4G2; BD Bioscience) plus 2% normal mouse and normal rat serum and stained with various combinations of the following Abs in FACS buffer (PBS containing 2% FBS and 2 mM EDTA) for 30 min at 4°C. Murine reactive antibodies, including B220-Pacific Blue, CD3-PerCPCy5.5, CD4-Pacific Blue, CD5-PerCPCy5.5, CD8-APC Cy, CD11b APC Cy7, CD21 FITC, CD23 PE, CD38-FITC, CD95 PE Cy7, CD138-PE, IgD APC Cy7, IgM FITC, GL-7 biotin, Streptavidin BV500, Ki67-APC, Ly6G PE Cy7, and Ly6C PerCP Cy5.5 were all purchased from BD Bioscience.
Katewa A., Suto E., Hui J., Heredia J., Liang J., Hackney J., Anderson K., Alcantar T.M., Bacarro N., Dunlap D., Eastham J., Paler-Martinez A., Rairdan X.Y., Modrusan Z., Lee W.P., Austin C.D., Lafkas D, & Ghilardi N. (2021). The peptide symporter SLC15a4 is essential for the development of systemic lupus erythematosus in murine models. PLoS ONE, 16(1), e0244439.
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