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119 protocols using hydrofluoric acid

1

Nitric Acid Digestion Protocol

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Nitric acid (69 % v/v), super purity grade was supplied from Romil, England. Hydrochloric acid (37 % v/v) and hydrofluoric acid (40 % v/v) were supplied from Merck (Germany). High purity water obtained from Millipore Milli-Q water purification system was used throughout the work.
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2

Determination of Lead (Pb) in Mouse Brain

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After completion of the behavioral experiment and blood collection, the mice were euthanized and their brain tissues were surgically removed, dissected, and preserved in normal saline. For the analytical determination of Pb, a 10% homogenate of the brain was prepared in Tris-HCl (50 mM). The homogenates were re-centrifuged at 17,000 rpm at 4 °C for 5 min and supernatants were filtered using 0.45 μm pore filters and analyzed by ICP–MS (Inductively Coupled Plasma Mass Spectrometer): ELAN 9000 (Perkin Elmer SciexInstrumento, Concord, Ontario, Canada). Nitric acid (69% v/v), super purity grade was supplied from Romil, Cambridge, UK. Hydrochloric acid (37% v/v) and hydrofluoric acid (40% v/v) were supplied by Merck (Darmstadt, Germany). High purity water obtained from the Millipore Milli-Q water purification system was used throughout the work. The ICP–MS calibration was carried out by external calibration with the blank solution and three working standard solutions (20, 40, and 60 ppm), starting from 1000 mg/L single standard solutions for ICP–MS (A ristar grade, BDH Laboratory Supplies, Poole, UK, for Cd).
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3

Nanoparticle Preparation for Leishmania Treatment

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Barley husk ash (donated by Altia Oyj), hydrofluoric acid (HF 38–40%, Merck), ethanol (EtOH 99.5%, Altia Oyj), hydrochloric acid (HCl 37–39%, Merck), sodium chloride (NaCl, Fisher Scientific UK), undecylenic acid (Merck), chloroform (Sigma-Aldrich), and Tween 20 (Sigma-Aldrich Chemie GmbH) were used in the preparation of the nanoparticles. Mannitol (Merck) was used as an excipient in the freeze-drying of the nanoparticles. Sodium acetate (NaOAc, Merck) and HPLC grade methanol (MeOH, VWR chemicals, Prolabo) were used in preparation of the mobile phase solution in HPLC analysis. Buparvaquone (BPQ) was prepared as described previously [12 (link)]. Amphotericin B (AmB), paromomycin (PMM), miltefosine (MF), medium 199, RPMI-1640, and fetal calf serum were purchased from Sigma Aldrich (USA). Sodium stibogluconate (SSG) was a generous gift from Albert David Ltd. (Kolkata, India). Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC 30–2002) and Fetal Bovine Serum (FBS, GE Healthcare Life Sciences) were used in cell culture.
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4

Gold Nanostructures Growth on Silicon Wafers

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Silicon wafers (p-type, 0.001–0.002 Ω cm, <100>) were purchased from Siegert Wafer GmbH (Germany). Hydrofluoric acid (48%) was supplied by Merck and ethanol (99.8%) by Carl Roth GmbH + Co. KG (Germany). The growth of gold nanostructures was carried out with ethanol (96 %) obtained from VWR International GmbH (Germany). HAuCl4 · 3 H2O (99.99%) was supplied by Alfa Aesar (Thermo Fisher (Kandel) GmbH, Germany). Toluene was also purchased from VWR International GmbH (Germany) and thiol terminated polystyrene (3,1400 g/mol) was obtained from Polymer Source, Inc (Canada).
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5

Analytical Techniques for Advanced Catalyst Characterization

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p.a. grade ethanol, hydrochloric acid, hydrofluoric acid, hydrogen peroxide solution and nitric acid were purchased from Merck (Germany, https://www.merckmillipore.com) and diluted with purified water prepared by a Barnstead EASYPURE II system (Thermo Fisher Scientific, USA, https://www.fishersci.de) as required. Single-element standards were purchased from Merck (Si, Al and Gd), SPEX (https://www.spexcertiprep.com) (U), VWR (https://www.vwr.com) (Sr and Au), ROTH (https://www.carlroth.com) (Ti) and Alfa Aesar (https://www.alfa.com) (Os). Liquid standard solutions with varying compositions and concentrations were prepared just before use by dilution of the respective single-element standards.
AUROlite™ (Strem Chemicals, https://www.strem.com) was used as a certified reference composition. For the synthesis of the custom catalyst material, rutile (TP Hombikat Mikrorutil, Venator, https://www.americanelements.com), anatase (Sigma-Aldrich, https://www.sigmaaldrich.com), gold(III) chloride (Sigma-Aldrich) and urea (Merck) were used. Makrolon® (Bayer Material Science, https://www.covestro.com) was selected as the substrate material for the μDD production and the analysis of all solid samples.
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6

Zeolite-Based Adsorbent Formulation

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The materials
utilized for the formulation and characterization are commercial zeolite
CBV 400 by Zeolyst (zeolite Y as HY, SiO2/Al2O3 = 5.1), CBV 5524G by Zeolyst (NH4-ZSM-5,
SiO2/Al2O3 = 50), and kaolin by Sigma-Aldrich.
As binders, sodium metasilicate (water glass) by Merck (27 wt % SiO2; 8 wt % Na2O), hydrated aluminum chloride by Alfa
Aesar (99 wt % AlCl3·6H2O), and aluminum
phosphate monobasic purum (95 wt %) by Sigma-Aldrich were used together
with LUDOX HS-40 (40 wt % SiO2; mass ratio SiO2/Na2O: 95/1) by Sigma-Aldrich. In addition, hydrochloric
acid (37%) from BDH Chemicals was added to control the pH value of
water glass slurries. Moreover, ammonium nitrate (99 wt %) from Grüssing
was used for post-treatment of the samples by ion exchange. Finally,
concentrated hydrochloric acid and nitric acid (69 vol % supra-quality)
from Carl Roth, hydrofluoric acid (40 vol %) from Merck, and boric
acid (99.995 wt %) by Alfa Aesar were used in the microwave-assisted
dissolution procedure for elementary analysis.
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7

Fabrication of Heterojunction Solar Cells

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n-Type silicon wafers (CZ, 1–10 Ω
cm) were purchased from Siegert Wafer GmbH, Germany. Fluorine-doped
tin oxide (FTO) with a sheet resistance of ∼25 Ω cm2 was purchased from Pilkington. Antimony chloride (SbCl3), zinc (Zn) dust, copper nitrate trihydrate (Cu(NO3)2·3H2O), silver nitrate (AgNO3), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), hexamethylenetetramine or hexamine (C6H12N4), lithium iodide (LiI), iodine (I2), poly(ethylene oxide) (PEO, MW: 600 000), N-[3-(trimethoxysilyl)propyl]aniline
(TMSPA), 3-propyl-1-methyl imidazolium iodide (PMII), 1-ethyl-3-methyl
imidazolium thiocyanate (EMISCN), hydrofluoric acid (HF, 40%), ammonia
(NH3, 32%), hydrogen peroxide (H2O2, 30%), toluene (C7H8), formic acid (H2CO2, 95%), hydrochloric acid (HCl, 37%), sulfuric
acid (H2SO4, 98%), ethanol, acetone, and isopropanol
were purchased from Merck. Ultrapure water with a resistivity of ∼18.2
MΩ cm was obtained from the Millipore Direct-Q3 UV system.
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8

Synthesis of Organic Compounds

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All used chemical materials in this study, aluminum chloride anhydrous (AlCl3, 95%), absolute methanol (MeOH, 99.0%), absolute ethanol (EtOH, 99.0%), 1,2-dichloroethane (DCE, 99.0%), dichloromethane (DCM, 99.5%), chloroform (99.5%), benzene (99.5%), Hydrofluoric acid (HF, 48%) with high purity have been purchased from Merck (Darmstadt, Germany).
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9

Monoclonal Antibody Characterization

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Trastuzumab (N1050H05; 21 mg/mL) was purchased from Evidentic GmbH (Berlin, Germany); mAb1 (21.7 mg/mL), mAb2 (18.5 mg/mL), and mAb3 (18.3 mg/mL) were kindly provided by Rentschler Biopharma SE (Laupheim, Germany). USP mAb003 (Cat. No. 1445595, LOT: F12980, 10 mg/mL), 3-(N-morpholino) propanesulfonic acid (MOPS)-buffer, 1,4-dithiothreitol (DTT), and hydrochloric acid (37%) were purchased from Sigma (Steinheim, Germany). IdeS protease was purchased from GENOVIS (FabRICATOR, 5000 units, Lund, Sweden). Urea (Ultrapure) and guanidine hydrochloride (99.5%) were purchased from Thermo Fisher Scientific (Dreieich, Germany). Isopropanol (IPA, LC–MS grade) and formic acid (FA, ≥ 98%) were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Sodium hydroxide (NaOH) and hydrofluoric acid (40%(v/v), HF) were purchased from Merck (Darmstadt, Germany). Polyethylene oxide (PEO, Mw: 1.000.000) was purchased from Alfa Aesar (Kandel, Germany).
Ultrapure water (18 MΩ*cm at 25 °C, SG Ultra Clear UV from Siemens Water Technologies, USA) was used for all solutions.
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10

Microwave-Assisted Metal Analysis

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For metal element analysis, the sampled filter paper is digested using 4 mL of nitric acid (Merck Ltd. Taiwan, Taipei, Taiwan, 65% GR for Analysis) and 2 mL of hydrofluoric acid (Merck Ltd. Taiwan, Taipei, Taiwan 48% Ultrapur) under microwave digestion at 200 °C for 15 min. The digestive solution was then analyzed using inductively coupled plasma mass spectrometry (ICP-MS) analysis. A total of 34 metals were analyzed including Al, Fe, Na, Mg, K, Ca, Sr, Ba, Ti, Mn, Co, Ni, Cu, Zn, Mo, Cd, Sn, Sb, Tl, Pb, V, Cr, As, Y, Se, Zr, Ge, Rb, Cs, Ga, La, Ce, Nd.
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