All mouse experimental procedures and protocols were evaluated and authorized according to the Beijing Regulations for Laboratory Animal Management. They were strictly in accordance with the guidelines of the Institutional Animal Care and Use Committee of China Agricultural University (approval number: SKLAB-2019-04-03). Nfatc1CreERT2 mice were generated at Shanghai Biomodel Organism Science & Technology Development Co., Ltd. R26mTmG mice and R26tdTomato mice were purchased from Jackson Laboratories (stock number: 007676, 007914). ProcrCreERT2-IRES-tdTomato mice were obtained from Zeng’s laboratory at the Chinese Academy of Sciences, Shanghai. Three-week old female NOD-SCID mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Female age-matched mice were utilized for all experiments. Eight-week old female mice were utilized for short-term labeling, long-term tracing and pregnancy experiments. Seven-week old female mice were used for ovariectomized and hormone stimulation.
R26mtmg mice
Manufactured by Jackson ImmunoResearch
The R26mTmG mice are a genetically modified mouse strain that expresses a fluorescent reporter system. This system utilizes a membrane-targeted tandem dimer Tomato (mT) cassette that is ubiquitously expressed, resulting in red fluorescent cells. Upon Cre-mediated recombination, the mT cassette is excised, and a membrane-targeted enhanced green fluorescent protein (mG) cassette is activated, causing cells to become green fluorescent.
Automatically generated - may contain errors
Lab products found in correlation
R26tdtomato mice, by Jackson ImmunoResearch (1 mentions)
Nod scid mice, by Charles River Laboratories (1 mentions)
Wnt1 cre mice, by Jackson ImmunoResearch (1 mentions)
Wt1 creert2 mice, by Jackson ImmunoResearch (1 mentions)
Ubc cre ert2 mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Janvier Labs (1 mentions)
5 protocols using r26mtmg mice
1
Mouse Lineage Tracing Protocols
2
Genetically Engineered Mouse Lines
3
Genotyping Transgenic Mouse Lines
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Tetracycline-regulated i-TERTci transgenic mice, WT1CreERt2 mice (The Jackson Laboratory, stock# 010912), R26mTmG mice (The Jackson Laboratory, stock# 007576), UBCCreERt2 mice (The Jackson Laboratory, stock# 008085), R26confetti mice (The Jackson Laboratory, stock# 013731), and TERTKO mice (The Jackson Laboratory, stock# 005423) were previously described13 (link),18 (link),27 (link),28 (link),43 (link)–45 (link). Mice were PCR-genotyped using the following oligonucleotide pairs: 5’-CGCCCAGAAGCTTGGTGTAG −3’, 5’-GCTCCATGGCGATGACTTAG-3’ (actin-rtTA + ); 5’-GGATGTACTTTGTTAAGGCAGCA-3’, 5’-ACAACGGAGTTCCTCAGTGC-3’ (tetop-TERTci + ); 5’-ATCGCAGGAGCGGAGAAC-3’, 5’-GCAAACGGACAGAAGCATTT-3’ (WT1CreERt2); 5’-CTCTGCTGCCTCCTGGCTTCT-3’, 5’-CGAGGCGGATCACAAGCAATA-3’, 5’-TCAATGGGCGGGGGTCGTT-3’ (R26mTmG); 5’-GACGTCACCCGTTCTGTTG-3’, 5’-AGGCAAATTTTGGTGTACGG-3’ (UBCCreERt2); 5’-GAATTAATTCCGGTATAACTTCG-3’, 5’-AAAGTCGCTCTGAGTTGTTAT-3’, 5’-CCAGATGACTACCTATCCTC-3’ (R26Confetti); 5’-CCCCAGGCGCCGCACAAAGG-3’, 5’- GGTCCTGGCTGTTTTCTAAG-3’, 5’-CTGGATTCATCGACTGTGGC-3’ (TERTKO).
4
Isolation and Lineage Tracing of Muscle Progenitor Cells
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Pax3GFP∕+ mice (Relaix et al., 2005 (link)) were used to isolate MPC by fluorescent activated cell sorting (FACS) of the GFP+ cells. Pax3Cre∕+ mutant mice were kindly provided by Jonathan A. Epstein (Engleka et al., 2005 (link)). R26mT−mG mice were obtained from The Jackson Laboratory (Stock No: 007576) (Muzumdar et al., 2007 (link)). For myofiber cultures C57BL/6J (Janvier®) male mice (8 weeks old) were used. For lineage tracing experiments, Pax3Cre∕+ mice were crossed with R26mT−mG to obtain Pax3Cre∕+; R26mT−mG double mutant mice.
All animals were maintained inside a barrier facility, and all in vivo experiments were performed in accordance with the French and European Community guidelines for the care and use of laboratory animals (Project No: 01427.03 approved by MESR and File No: 15-018 from the Ethical Committee of Anses/ENVA/UPEC).
All animals were maintained inside a barrier facility, and all in vivo experiments were performed in accordance with the French and European Community guidelines for the care and use of laboratory animals (Project No: 01427.03 approved by MESR and File No: 15-018 from the Ethical Committee of Anses/ENVA/UPEC).
5
Mouse Embryonic Staging and Genotyping
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Pdgfrb‐Cre mice (Foo et al.,2006 ) were kindly provided by Ralf Adams (Max Planck Institute for Biomedicine, Münster, Germany). R26‐mTmG mice (Muzumdar et al., 2007 ); obtained from the Jackson Laboratory) and Tie2‐Cre mice (Koni et al., 2001 ) were described previously. Staging of E9 and E10 embryos were done by somite counting (sc) and Theiler stage (TS) was determined according to EMAP eMouse Atlas Project (http://www.emouseatlas.org ) (Richardson et al., 2014 ). Data from E9 embryos refer to sc 15‐18, TS14; E10 sc 24‐26, TS15; and E10.5 sc 34‐37, TS17. For embryos older than E11, the morning of vaginal plug detection was considered as E0. All strains were maintained and analyzed on C57BL/6J background.
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Pdgfrb‐Cre mice (Foo et al.,
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