Fisherbrand superfrost plus microscope slide

Manufactured by Thermo Fisher Scientific

Sourced in United States

Fisherbrand Superfrost Plus Microscope Slides are glass slides designed for use in microscopy applications. They provide a clean, uniform surface for mounting and analyzing samples.

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Lab products found in correlation

Dako autostainer link 48 system, by Agilent Technologies (6 mentions) Tissue tek oct compound, by Sakura Finetek (4 mentions) Cryostat, by Leica (3 mentions) Dako pd l1 ihc 22c3 pharmdx kit, by Agilent Technologies (3 mentions) Microm hm 525 cryostat, by Thermo Fisher Scientific (2 mentions) Tissue tek optimum cutting temperature compound, by Sakura Finetek (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) C1 confocal microscope, by Nikon (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Lsm800 confocal microscope, by Zeiss (2 mentions) Pbs dapi, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (2 mentions) Flex visualization system, by Agilent Technologies (2 mentions) Rm2155 microtome, by Leica (2 mentions) R37121, by Thermo Fisher Scientific (1 mentions) Fluormount g, by Electron Microscopy Sciences (1 mentions) Bx41 phase contrast darkfield microscope, by Olympus (1 mentions) Basescope duplex detection reagent kit, by Advanced Cell Diagnostics (1 mentions) Dp25 5mp color firewire camera, by Olympus (1 mentions) Prolong diamond antifade, by Thermo Fisher Scientific (1 mentions)

43 protocols using fisherbrand superfrost plus microscope slide

Rat Brain Immunostaining for HDAC5

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Rats were deeply anesthetized with isoflurane and perfused transcardially with 500 ml of 0.01 m PBS. Brain tissue was then fixed with 500 ml of 4% paraformaldehyde (PFA) for 1 h before being transferred into 30% sucrose PBS solution. Once the brains sank, they were sectioned into 30 μm slices using a Leica cryostat and stored in cryoprotectant at −80°C. For HDAC5 immunohistochemistry, the sections were washed for 10 min in PBS and then incubated for 1 h at room temperature in blocking buffer (2% BSA in PBS with 0.3% Triton X-100). The sections were incubated next with a primary antibody against HDAC5 (1:500; catalog #sc-133106, Santa Cruz Biotechnology; RRID:AB_2116793) in blocking buffer overnight at room temperature. After washing the sections three times in PBS (5 min each), they were incubated with the secondary antibody Alexa 594-labeled anti-mouse (1:200; catalog #R37121, Thermo Fisher Scientific; RRID:AB_2556549) in blocking buffer for 1 h at room temperature. Finally, the sections were washed in PBS and mounted on glass slides (Fisherbrand Superfrost Plus Microscope Slides, catalog #12-550-15, Fisher Scientific) that were air dried and cover slipped with Fluormount G (Electron Microscopy Sciences).

Pribut H.J., Vázquez D., Wei A.D., Tennyson S.S., Davis I.R., Roesch M.R, & Li X. (2021). Overexpressing Histone Deacetylase 5 in Rat Dorsal Striatum Alters Reward-Guided Decision-Making and Associated Neural Encoding. The Journal of Neuroscience, 41(49), 10080-10090.

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Cryosectioning and in situ mRNA Detection in Exaiptasia

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Adult Exaiptasia were paralyzed in anesthetic solution (15% MgCl₂), rinsed in PBS, then embedded in Tissue-Tek O.C.T. Compound (Sakura Cat #4583) in cryomolds (Sakura Tissue-Tek® Cryomold®, Intermediate, Cat #4566) and flash frozen on dry ice and stored at −80°C. Cryostat sections (18–20μm) were adhered to Fisherbrand™ Superfrost™ Plus Microscope Slides (Fisher Scientific Cat #12–550-15) and flash frozen on dry ice and stored at −80°C until used for BaseScope. The BaseScope Duplex Detection Reagent Kit (Advanced Cell Diagnostics Cat #323800) and the manufacturer’s manual (BaseScope Duplex Detection Reagent User Manual, ACDBio) was followed to hybridize custom probes or positive control (Cat #700101) or negative control probes (Cat #700141) to targets in tissue cryosections and amplify signals. Samples were imaged on an Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Cat #BX41-PH-B) and images were acquired using the Olympus CellSens software and Olympus DP25 5MP Color Firewire Camera.

He L.S., Qi Y., Allard C.A., Valencia-Montoya W.A., Krueger S.P., Weir K., Seminara A, & Bellono N.W. (2023). Molecular tuning of sea anemone stinging. bioRxiv.

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Immunohistochemical Analysis of Mammary Tissue

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Mammary fat pads or EO771 tumors were isolated and fixed in 2% paraformaldehyde solution for 2 hours at room temperature. Tissues were subsequently dehydrated in 30% sucrose solution overnight before being frozen in Tissue-Tek^® “optimal cutting temperature” (O.C.T.) compound (#4583, Sakura). Seven-micron sections were mounted onto Fisherbrand^™ Superfrost^™ Plus microscope slides (12–550-15, Fisher Scientific) using a Cryostat (CM1860, Leica) set to −30°C. Sections were secondarily fixed in acetone for 10 minutes at 20°C, air dried, and rehydrated with PBS. Slides were blocked with 5% BSA for 1 hour and stained with primary antibodies before nuclear staining with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI). After staining slides were mounted with ProLong^™ Diamond Antifade (P36970, Thermo Fisher Scientific) and imaged on a Leica DM6000 B microscope. Adobe Photoshop was used for image processing.

Gavil N.V., Scott M.C., Weyu E., Smith O.C., O’Flanagan S.D., Wijeyesinghe S., Lotfi-Emran S., Shiao S.L., Vezys V, & Masopust D. (2023). Chronic antigen in solid tumors drives a distinct program of T cell residence. Science immunology, 8(84), eadd5976.

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Tissue Preparation for Brain Injury Studies

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Both injured and sham control swine were euthanized at the post injury time points noted above as previously described [32 (link), 33 (link)]. Briefly, transcardial perfusion was performed under surgical plane anesthesia using heparinized saline (2L) followed by 10 % neutral buffered formalin (NBF) (8L). The brain was removed, extracted, and post-fixed in 10% NBF for 1 week. Thereafter, the brains were sectioned at 5mm in the coronal plane, with tissue sections processed for standard paraffin embedding in an automated tissue processor (Shandon Scientific Instruments, Pittsburgh, PA). Serial sections (8 μm) were cut on a Leitz rotary microtome (Leica, Malvern, PA) then mounted on Fisherbrand Superfrost/Plus microscope slides (Fisher Scientific, Pittsburgh).
For human brain tissue preparation, the intact brain was immersed in 10% formol saline at autopsy and fixed for at least 3-weeks prior to dissection. Sampling was achieved using a standardized protocol and paraffin embedding was performed as described previously [24 (link)]. The region of cingulate gyrus including corpus callosum was selected in this study given its midline location containing white matter and known susceptibility to injury [1 (link), 2 (link), 29 (link), 31 (link), 32 (link), 36 (link)].

Song H., McEwan P.P., Ameen-Ali K.E., Tomasevich A., Kennedy-Dietrich C., Palma A., Arroyo E.J., Dolle J.P., Johnson V.E., Stewart W, & Smith D.H. (2022). Concussion leads to widespread axonal sodium channel loss and disruption of the node of Ranvier. Acta neuropathologica, 144(5), 967-985.

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Mouse Skin Sectioning and Preservation

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Glabrous skin from the hindpaw and hairy skin from the back of the mouse were depilated with Surgi Cream-Extra Gentle for Face (Ardell, Los Angeles, CA, USA) and removed by blunt-dissection. Skin pieces were spread on an index card with the epidermal surface up, fixed in 4% PFA for 20 minutes, washed three times in phosphate-buffered saline (PBS) for 5 minutes/wash, then sunk in 30% sucrose in PBS for 24h. The skin was removed, embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), and frozen. Samples were cut in a cross-sectional plane at 25μm thickness using a Microm™ HM 525 Cryostat (Thermo Scientific, Waltham, MA, USA) and were thaw mounted onto Fisherbrand Superfrost Plus microscope slides (Fisher Scientific, Pittsburgh, PA, USA). Slides with sections were kept at −80°C until use.

Cabañero D., Irie T., Celorrio M., Trousdale C., Owens D.M., Virley D., Albrecht P.J., Caterina M.J., Rice F.L, & Morón J.A. (2016). Identification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory abnormalities. Pain Reports, 1(3), e573.

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Fiber-type specific satellite cell analysis

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Immunohistochemical techniques were conducted as previously described (Fry et al. 2014b, 2016). Samples were removed from cork at −25°C in a cryostat (Thermo HM525‐NX) where serial sections were cut (7 μm). Pre and post samples for the same subject were placed on the same Fisherbrand Superfrost^®/Plus microscope slides (Fisher Scientific) for analysis of; (1) fiber‐type specific satellite cell (Pax7⁺) content and myonuceli, (2) double stain of proliferative (Ki67⁺) satellite cells (Pax7⁺) and (3) proliferative MyoD+ cells. Following cutting, hydrophobic marker was used to separate the sections, dried at room temperature and then stored at −20^°C until analysis. Only 9 of the +EAA and 5 of the −EAA subjects had suitable muscle cross‐sections for analysis.

Reidy P.T., Fry C.S., Dickinson J.M., Drummond M.J, & Rasmussen B.B. (2017). Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Physiological Reports, 5(11), e13269.

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Comprehensive Tumor Characterization Protocol

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Tumors were harvested at sacrifice at day 20, weighed and either snap-frozen, and processed for histology, immunohistochemistry, immunoblot, and flow cytometry analyses. Tumors were paraffin-embedded and 5 μm sections were placed on Fisherbrand Superfrost Plus Microscope Slides (Fisher Scientific), deparaffinized, treated with Retrievit-6 Target Retrieval Solution (BioGenex), and processed for immunofluorescence and histological analyses (see below). Frozen tumor tissues were used for immunoblot analyses. In addition, portions of mouse tumors were minced and digested with 0.1% collagenase I (Worthington) at 37 °C for 1 h. Cells were passed through 70 μm mesh and washed twice, and the resulting single-cell suspension was used for flow cytometric analysis and sorting.

Kartha V.K., Alamoud K.A., Sadykov K., Nguyen B.C., Laroche F., Feng H., Lee J., Pai S.I., Varelas X., Egloff A.M., Snyder-Cappione J.E., Belkina A.C., Bais M.V., Monti S, & Kukuruzinska M.A. (2018). Functional and genomic analyses reveal therapeutic potential of targeting β-catenin/CBP activity in head and neck cancer. Genome Medicine, 10, 54.

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Epididymis Tissue Fixation and Cryosectioning

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Epididymis tissues were fixed by immersion in a paraformaldehyde–lysine–periodate solution containing 4% v/v paraformaldehyde, 75 mM lysine, 10 mM sodium periodate and 5% w/v sucrose in 100 mM phosphate buffered saline (PBS) at room temperature for 4 h, or overnight at 4 °C. Tissues were washed with PBS three times and stored in 0.02% sodium azide–PBS. Cryosections of 5 and 16 μm thickness were collected from epididymides that were cryoprotected in 30% sucrose overnight at 4 °C and embedded in Tissue-Tek optimum cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA). Cryosections were cut using a Leica CM3050-S microtome (Leica Microsystems, Buffalo, IL) and collected onto Fisherbrand Superfrost Plus microscope slides (Fisher Scientific, Pittsburg, PA).

Roy J., Kim B., Hill E., Visconti P., Krapf D., Vinegoni C., Weissleder R., Brown D, & Breton S. (2016). Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging. Nature Communications, 7, 10666.

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Mouse Skin Tissue Processing for Histology

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Glabrous skin from the hind paw and hairy skin from the back of the mouse were depilated with Surgi Cream-Extra Gentle for Face (Ardell, Los Angeles, CA) and removed by blunt-dissection. Skin pieces were spread on an index card with the epidermal surface up, fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich, Saint Louis, MO) for 20 minutes, washed 3 times in phosphate-buffered saline (PBS) for 5 minutes/wash, then sunk in 30% sucrose in PBS for 24 hours. The skin was removed, embedded in Tissue-Tek optimum cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA), and frozen. Samples were cut in a cross-sectional plane at 25 μm thicknesses using a Microm HM 525 Cryostat (Thermo Scientific, Waltham, MA) and were thaw mounted onto Fisherbrand Superfrost Plus microscope slides (Fisher Scientific, Pittsburgh, PA). Slides with sections were kept at −80°C until use.

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Immunohistochemical Analysis of Inner Ear Tissues

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Ten micrometer
sections were cut
parallel to the modiolus, mounted on Fisherbrand Superfrost/Plus Microscope
Slides (Fisher Scientific, Pittsburgh, PA) and dried overnight. The
samples were deparaffinized and rehydrated in PBS two times for 5
min, then three times in 0.2% Triton X-100 in PBS for 5 min, and finally
in blocking solution 0.2% Triton X-100 in PBS with 10% fetal bovine
serum for 30 min at room temperature. After blocking, specimens were
treated with antibodies (Table 8) diluted to concentration with a blocking solution. The tissue
was incubated for 48 h at 4 °C in a humid chamber. After three
rinses in 0.2% Triton X-100 in PBS, immunohistochemical detection
was carried out using an Alexa Fluor Donkey 555 anti-Rabbit. The secondary
antibody was incubated for 6 h at room temperature in a humid chamber.
The slides were rinsed in 0.2% Triton X-100 in PBS three times for
5 min and finally coverslipped with the ProLong Gold antifade reagent
(Invitrogen Molecular Probes, Eugene, OR). Imaging was carried out
with a Nikon C-1 confocal microscope.

Schmitt H.A., Pich A., Prenzler N.K., Lenarz T., Harre J., Staecker H., Durisin M, & Warnecke A. (2021). Personalized Proteomics for Precision Diagnostics in Hearing Loss: Disease-Specific Analysis of Human Perilymph by Mass Spectrometry. ACS Omega, 6(33), 21241-21254.

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