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Insulin transferrin selenium mix

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The Insulin-transferrin-selenium mix is a laboratory reagent used to supplement cell culture media. It contains insulin, transferrin, and selenium, which are essential components for the growth and proliferation of cells in vitro.

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Lab products found in correlation

Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Nicotinamide, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Nci h211, by American Type Culture Collection (1 mentions) Nci h841, by American Type Culture Collection (1 mentions) Nci h1048, by American Type Culture Collection (1 mentions) Hcc33, by American Type Culture Collection (1 mentions) Nci 146, by American Type Culture Collection (1 mentions) Antibiotic antimycotic mix, by Thermo Fisher Scientific (1 mentions) Lncap, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Insulin (904 citations) Insulin-transferrin-selenium (622 citations) Transferrin (151 citations) Selenium (75 citations)

3 protocols using insulin transferrin selenium mix

1

Hepatocyte Differentiation from Stem Cells

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Stem cells at P2 were preconditioned with Iscove's modified Dulbecco's medium (IMDM; Gibco; Thermo Fisher Scientific, Inc.) containing 20 ng/ml epidermal growth factor and 10 ng/ml bFGF, following which they were differentiated into hepatocytes using a two-step protocol (13 (link)). The cells were then treated with differentiation medium, consisting of IMDM supplemented with 20 ng/ml hepatocyte growth factor (Thermo Fisher Scientific, Inc.), 10 ng/ml bFGF (Thermo Fisher Scientific, Inc.) and 0.61 g/l nicotinamide (Sigma-Aldrich; Merck KGaA) for 7 days. Subsequently, cells were transferred to maturation medium, consisting of IMDM with 20 ng/ml oncostatin M, 1 µmol/l dexamethasone and 50 mg/ml insulin-transferrin-selenium mix (all from Thermo Fisher Scientific, Inc.). The obtained cells were maintained in this medium (13 (link)).
Radhakrishnan S., Trentz O.A., Martin C.A., Reddy M.S., Rela M., Chinnarasu M., Kalkura N, & Sellathamby S. (2019). Effect of passaging on the stemness of infrapatellar fat pad-derived stem cells and potential role of nucleostemin as a prognostic marker of impaired stemness. Molecular Medicine Reports, 20(1), 813-829.
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2

Culturing SCLC Cell Lines for Research

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The human SCLC cell lines NCI-H841, NCI-H1048, NCI-889, NCI-2171, NCI-146, NCI-H1963, HCC33 and NCI-H211 were obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were grown in RPMI 1640 with 10% FetalClone (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C with 5% CO2 in a humidified incubator, except for NCI-H2171 and NCI-H889 cells, which were grown in a HITES medium (DMEM/F12 supplemented with 1% Glutamax, 100 U/mL penicillin, 100 µg/mL streptomycin, 4 µg/mL hydrocortisone (Sigma, St Louis, MO, USA), 5 ng/mL murine EGF and 1% of an insulin–transferrin–selenium mix (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 5% FetalClone. Cell lines were authenticated and routinely tested for mycoplasma.
Carazo F., Bértolo C., Castilla C., Cendoya X., Campuzano L., Serrano D., Gimeno M., Planes F.J., Pio R., Montuenga L.M, & Rubio A. (2020). DrugSniper, a Tool to Exploit Loss-Of-Function Screens, Identifies CREBBP as a Predictive Biomarker of VOLASERTIB in Small Cell Lung Carcinoma (SCLC). Cancers, 12(7), 1824.
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3

Immortalized Human Prostate Cell Lines

Cited 1 time
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Prostate stromal (BHPrS-1) and epithelial (NHPrE-1 and BHPrE-1) cells were gifts from Dr. Simon Hayward (NorthShore Research Institute, Evanston, IL). NHPrE-1 and BHPrE-1 lines were generated from primary cells by spontaneous immortalization, whereas BHPrS-1 were immortalized using human telomerase reverse transcriptase (hTERT)30 (link). THP-1 leukemia, LNCaP, and 22RV1 prostate cancer lines were purchased from ATCC. C4–2B31 (link) cells were provided by Drs. Ruoxiang Wu and Leland Chung (Cedars-Sinai, Los Angeles, CA). NHPrE-1 and BHPrE-1 cells were cultured in Dulbeccòs Modified Eaglès Medium (DMEM):F12 in 1:1 ratio supplemented with 0.4% bovine pituitary extract, insulin-transferrin-selenium mix, epidermal growth factor (10ng/ml), antibiotic-antimycotic mix and 5% FBS (Thermo Fisher Scientific). THP-1, LNCaP, C4–2b and 22Rv1 cells were cultured in Roswell Park Memorial Institute-1640 (RPMI) medium containing 10% fetal bovine serum (FBS) and BHPrS-1 cells with 5% FBS.
Popovics P., Awadallah W.N., Kohrt S.E., Case T.C., Miller N.L., Ricke E.A., Huang W., Ramirez-Solano M., Liu Q., Vezina C.M., Matusik R.J., Ricke W.A, & Grabowska M.M. (2020). Prostatic osteopontin expression is associated with symptomatic benign prostatic hyperplasia. The Prostate, 80(10), 731-741.
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