Sequoia complete stranded rna library prep kit

RNA‐Seq library preparation was performed starting from 200 ng total RNA using the SEQuoia Complete Stranded RNA Library Prep Kit (Bio‐Rad Laboratories, Inc.), which permits the capture of long as well as short RNAs in a single library. Two pools of 11 samples were prepared, and sequencing was performed on one SP flow cell on an Illumina NovaSeq 6000 instrument (Functional Genomic Center Zurich). Paired‐end sequencing was performed with 92 bp for read one (cDNA insert) and 8 bp for read 2 (UMI sequence for removal of PCR duplicates) and revealed between 24 and 42 million reads per library.

RNA-Seq library preparation was performed starting from 27 ng total RNA by using the SEQuoia Complete Stranded RNA Library Prep Kit (Bio-Rad Laboratories AG, Cressier, Switzerland) following manufacturer´s instructions. To deplete ribosomal RNA fragments, QIAseq FastSelect depletion kit (QIAGEN AG) was used in the first step of the library preparation. Sequencing of the 26 libraries was conducted on an Illumina NovaSeq 6000 instrument at the Functional Genomics Center Zurich (FGCZ). Pooled barcoded libraries were run on one SP flow cell. Paired-end sequencing was performed with 92 bp for read one (cDNA insert) and 8 bp for read 2 (UMI sequence for removal of PCR duplicates) and revealed between 21 and 44 million reads per library.