Global chemical analyses of the wine were performed by the Natoli laboratory (St Clément de Rivière, France) according to OIV procedures (www.oiv.int ). Analyses included: alcoholic percentage (Fourier transformed infrared spectroscopy—FTIR); glucose and fructose (FTIR–Foss wine scan auto); total acidity (FTIR–Foss wine scan auto); volatile acidity; free, active and total sulfur dioxide (automated colorimetric method); pH; malic and lactic acid (FTIR–Foss wine scan auto); total polyphenols index (FTIR–Foss wine scan auto); CO2 (FTIR–Foss wine scan auto); Fe (Colorimetric method, reaction with disodium salt of (pyrildil-2)-3bis(phenyl-4-sulfonic 5–6 triazin-1,2,3,4) acid); absorbance read at 570 nm on a sequential analyzer (Olympus AU2700); Cu (colorimetric method 4-(3,5-Dibromo-2-Pyridilazo)-N-Ethyl-N-(3-Sulfopropyl)Aniline reaction; absorbance read at 570 nm on a sequential analyzer (Olympus AU2700). (supplementary data: Tables S1–S3 ).
Au2700
Manufactured by Olympus
Sourced in Japan, Germany, United States, France, China
The AU2700 is a fully automated clinical chemistry analyzer designed for high-throughput laboratory testing. It offers a wide range of analytical capabilities, including photometric, ion-selective, and enzymatic assays. The AU2700 is capable of performing a variety of clinical chemistry tests, such as those for metabolic, renal, and liver function.
Automatically generated - may contain errors
Lab products found in correlation
58500 bp recorder, by Ugo Basile (3 mentions)
Insulin elisa kit, by Mercodia (2 mentions)
Cobas 8000, by Roche (2 mentions)
Cobas e601, by Roche (2 mentions)
Cobas c701, by Roche (2 mentions)
Cobas c701 702, by Roche (2 mentions)
Clinical chemistry reagent kits, by Randox (1 mentions)
Bc 6800, by Mindray (1 mentions)
Dkk100, by R&D Systems (1 mentions)
High performance liquid chromatography system, by Bio-Rad (1 mentions)
Architect i2000sr, by Abbott (1 mentions)
Abi 7500, by Thermo Fisher Scientific (1 mentions)
Colorimetric assay kit, by Nanjing Jiancheng (1 mentions)
Safire2, by Tecan (1 mentions)
High performance liquid chromatography (hplc), by Bio-Rad (1 mentions)
Eclia, by Roche (1 mentions)
K 4500, by Sysmex (1 mentions)
Nefa c test wako, by Fujifilm (1 mentions)
Advia centaur cp system, by Siemens (1 mentions)
Ab8152, by Abcam (1 mentions)
140 protocols using au2700
1
Comprehensive Wine Chemical Analysis
2
Enological Analysis of Studied Wines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Studied wines were characterized with enological usual chemical analyses (Natoli laboratory, St Clément de Rivière, France) according to OIV procedures (www.oiv.int , accessed on 23 November 2021). Analyses included: alcoholic percentage (Fourier transformed infrared spectroscopy: FTIR); total acidity (FTIR); volatile acidity; total sulfur dioxide (automated colorimetric method); pH (FTIR); Fe (colorimetric method, reaction with disodium salt of (pyrildil-2)-3bis(phenyl-4-sulfonic 5–6 triazin-1,2,3,4) acid), absorbance read at 570 nm on a sequential analyzer (Olympus AU2700); Cu (colorimetric method 4-(3,5-Dibromo-2-Pyridilazo)-N-Ethyl-N-(3-Sulfopropyl) aniline reaction, absorbance read at 570 nm on a sequential analyzer (Olympus AU2700) (Supplementary Data Table S1 ).
3
Pleural Effusion Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 10 mL sample of pleural effusion was collected at the time of presentation from each group 1 dog via ultrasonographic-guided thoracentesis. Fluid samples were transferred in plastic tubes with K3-EDTA for the determination of haematocrit and total nucleated cell count, and in plain glass tubes for the determination of total protein with an automated chemistry analyzer (Olympus AU 2700, Olympus Diagnostics, Hamburg, Germany). A board certified clinical pathologist performed the cytology examinations on all fluid samples. Fluid samples were also transferred in a 3.5 mL plastic tubes with 3.2% sodium citrate (final ratio of volume of anticoagulant to volume of blood, 1:9) (3.2% sodium citrate Vacuette® 3.5 mL, Grenier Bio-One, Kremsmünster, Austria) for the measurement of coagulation variables (see next paragraph).
4
Comprehensive Liver Function Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The assessment of liver function was performed by determining specific serum biomarkers, including aspartate transaminase (AST), alanine transaminase (ALT), total cholesterol (CHO), triglycerides (TG), alkaline phosphatase (ALP), high-density lipoprotein-cholesterol (HDL), and low-density lipoprotein-cholesterol (LDL), were conducted by using clinical chemistry reagent kits (Randox Laboratories Ltd., Antrim, UK) according to the manufacturer's institutions. Plasma samples were acquired by collecting blood using EDTA tubes and then centrifuged at 1500 x g for 10 min at 4°C. The resulting supernatant was transferred into a new tube and used as a plasma sample. Determination of biochemical factors in plasma was conducted by enzyme-coupling reactions and colorimetric measurement using an automatic analyzer (Olympus AU2700, Olympus Co., Tokyo, Japan).
5
Biochemical Markers of Cardiometabolic Health
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The serum sample was collected using ethylenediaminetetraacetic acid (EDTA) tubes and centrifuged at 3000 rpm (1400 g) for 10 min at 4 °C. Concentrations of glucose, TGs, total cholesterol, LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), FFA, aspartate aminotransferase (AST), alanine aminotransferase (ALT) were measured by enzymatic colorimetric methods using commercial kits (Randox Laboratories, Ltd., Antrim, UK). The analysis of serum was carried out by an automatic analyzer (Olympus AU2700, Olympus Co., Tokyo, Japan).
6
Comprehensive Health Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A questionnaire was used and face-to-face interview was conducted by uniformly trained investigators to collect personal information, disease history, family history, and lifestyle behavior. During the survey, height, weight, waist circumference (WC), systolic (SBP), and diastolic blood pressure (DBP) were measured and recorded, according to standardized methods. The total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting plasma glucose (FPG), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol(LDL-C) were measured using automatic biochemical analyzer (Olympus Au 2700; Olympus diagnostics, Hamburg, Germany). Each subject signed an informed consent form. The investigation was approved by the ethics review committee of the First A liated Hospital of Shihezi University Medical College (IERB No. SHZ2010LL01), and its operations and methods were carried out in accordance with the relevant guidelines.
7
Biochemical Profiling of Adipose Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of plasma total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and glucose were analyzed using an automated autoanalyzer (Olympus Au2700; Olympus Corporation, Tokyo, Japan). A total of 30 mg epididymal fat was homogenized at 4°C following the addition of 1 ml PBS. The supernatant was carefully collected following centrifugation for 10 min at 1,200 × g at 4°C. Leptin (cat. no. KRC2281), adiponectin (cat. no. KRP0041), interleukin-6 (IL-6; cat. no. BMS625), tumor necrosis factor-α (TNF-α; cat. no. 88-7340-86) and monocyte chemoattractant protein-1 (MCP-1; cat. no. BMS631INST) in the plasma and the supernatant of the epididymal fat were determined using commercially available ELISA kits (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
8
Hepatic Function and Serum Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hepatic function tests: A portion of the liver (500 mg) was used for lipid extraction. The tissues were homogenized in phosphate-buffered saline, and the lipids were extracted using n-hexane-isopropanol (3:2, v/v). Once dry, the lipid extracts were redissolved in 200 μL of isopropanol, and a small volume was used to measure the cholesterol and triglyceride concentrations through enzymatic assays. The activity of the antioxidant enzymes (GSH, GPx, SOD, catalase, GST-α, and total GST assay) was assayed using commercial kits (Randox Laboratories, Antrim, UK). The enzymes were measured using enzymatic colorimetric methods, according to the manufacturer’s protocols.
Serum analysis: The concentrations of the total triglyceride (TG), total cholesterol (TC), leptin, adiponectin, AST, ALT, γGT, AFP, TNF-α, and IL-6 were measured using enzymatic colorimetric methods with commercial kits (Randox Laboratories) according to the manufacturer’s protocols. The serum analyses were conducted using an automatic analyzer (Olympus AU2700, Olympus, Tokyo, Japan).
Serum analysis: The concentrations of the total triglyceride (TG), total cholesterol (TC), leptin, adiponectin, AST, ALT, γGT, AFP, TNF-α, and IL-6 were measured using enzymatic colorimetric methods with commercial kits (Randox Laboratories) according to the manufacturer’s protocols. The serum analyses were conducted using an automatic analyzer (Olympus AU2700, Olympus, Tokyo, Japan).
9
Oxidative Stress and Inflammation Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum total oxidative stress (TOS), TAC levels, hs-CRP, gamma-glutamyltransferase (GGT), and uric acid (UA) levels were measured at baseline. Decreased TAC and increased TOS and UA levels were used as markers of oxidative stress, and increased hs-CRP was used as a marker of inflammation. Laboratory analysis was performed as stated in the previous study12 (link)–14 (link)
. TAC and TOS levels were determined with a spectrophotometric kit (Rel Assay Diagnostics, Gaziantep, Turkey) and read in an auto-analyzer (Olympus AU2700; Olympus, Tokyo, Japan). The TAC and TOS levels were expressed as mmol Trolox equivalent/L and mmol H2O2 equivalent/L, respectively. The oxidative status index (OSI) is defined as the ratio of TOS to TAC levels, expressed as a percentage. For the calculation of OSI, TAC units were represented as mmol/L, and the OSI value calculated according to the following formula: OSI (arbitrary unit) = TOS (mmol H2O2 equiv./L)/TAC (mmol Trolox equiv./L)12 (link)–14 (link)
.
. TAC and TOS levels were determined with a spectrophotometric kit (Rel Assay Diagnostics, Gaziantep, Turkey) and read in an auto-analyzer (Olympus AU2700; Olympus, Tokyo, Japan). The TAC and TOS levels were expressed as mmol Trolox equivalent/L and mmol H2O2 equivalent/L, respectively. The oxidative status index (OSI) is defined as the ratio of TOS to TAC levels, expressed as a percentage. For the calculation of OSI, TAC units were represented as mmol/L, and the OSI value calculated according to the following formula: OSI (arbitrary unit) = TOS (mmol H2O2 equiv./L)/TAC (mmol Trolox equiv./L)12 (link)–14 (link)
.
10
Serum Lipid and Glucose Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The serum levels of TC, TG, FFA, LDL-C, HDL-C, and glucose were determined by enzymatic colorimetric methods using commercial kits (Randox Laboratories, Ltd., Antrim, UK) according to the manufacturer's protocol. The analysis of serum biochemical parameters was carried out by an automatic analyzer (Olympus AU2700, Olympus Co., Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!