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Beckman glucose analyser

Manufactured by Beckman Coulter
Sourced in United States

The Beckman Glucose Analyser is a laboratory instrument designed to measure glucose levels in biological samples. It provides accurate and reliable glucose concentration data to support clinical diagnosis and monitoring.

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4 protocols using beckman glucose analyser

1

Defining Diabetes, Obesity, and Hypertension Metrics

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Participants with fasting blood glucose >125 mg/dL during the examination or who reported taking anti-diabetic medication were defined as having diabetes, according to the American Diabetes Association guidelines for the diagnosis of type-2 diabetes mellitus [18 (link)]. Blood glucose levels (mg/dL) were measured with a Beckman Glucose Analyser (Beckman Instruments, Fullerton, CA, USA). Obesity was defined as a body mas index (BMI) greater than 29.9 Kg/m2 [19 (link)]. Arterial blood pressure was measured three times, with the participant in a sitting position and being at rest for at least 30 min. Participants who had an average blood pressure of ≥140/90 mmHg or were under anti-hypertensive medication were classified as being hypertensive. Hypercholesterolemia was defined as having total cholesterol levels >200 mg/dL or the use of lipids-lowering agents [20 (link)].
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2

Plasma Biomarker Measurements Protocol

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Plasma glucose concentration was determined by the glucose oxidase method on a Beckman Glucose Analyser (Beckman Instruments, Fullerton, CA, USA). Plasma insulin was determined by radioimmunoassay (Diagnostic Products, Los Angeles, CA, USA). HbA1c was measured by affinity chromatography (Biochemical Methodology, Drower 4350; Isolab, Akron, OH, USA) and plasma free fatty acids by enzymatic colorimetric quantification (Wako Chemicals, Neuss, Germany). Plasma total cholesterol and triglyceride were measured enzymatically (Boehringer-Mannheim, Indianapolis, IN, USA) on a Hitachi 704 autoanalyser. Plasma HDL cholesterol was measured enzymatically on Hitachi 704 autoanalyser after precipitation of chylomicron, and VLDL and LDL cholesterol by phosphotungstic acid. LDL cholesterol was calculated from the Friedewald equation. Plasma adiponectin was measured by radioimmunoassay (Linco Research, St Charles, MO, USA).
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3

Plasma Biomarker Analysis in Mice

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Blood samples were obtained by cut tip from tail vein of conscious mice at the time points indicated in the figures and plasma was separated by centrifugation at 16,060×g for 3 min at 4 °C. Plasma glucose was measured using an automated glucose oxidase procedure with a Beckman glucose analyser (Beckman-Coulter, High Wycome, UK) and plasma insulin was determined by radioimmunoassay (Flatt and Bailey, 1981) . Insulin content of resected pancreatic tissue was also assayed after extraction with acid ethanol (HCl
total-GIP (Millipore) and C-reactive protein (Abcam) were assessed using ELISA as per manufacturers' instructions. Amylase Assay Kit (Abcam) was used to quantify plasma amylase activity as per manufacturers' instructions. DPP-IV activity was evaluated by Gly-Pro-AMC cleavage as previously described (Davis et al., 2010 , McCloskey et al. 2020) . Plasma triacylglycerol and cholesterol levels were measured using a Hitachi Automated Analyser 912 (Mannheim, Germany).
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4

Blood Glucose and Clotting Factors

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PG was obtained by glucose oxidase method using a Beckman Glucose Analyser (Beckman Instruments). PI was measured by radioimmunoassay (INEP-Zemun) double antibody kits. Plasma PAI-1 activity was evaluated by plasminogen chromogenic plasmin substrate assay (Boehringer).
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