Paraffin wax

The specimens were fixed using 10% formalin (Solarbio, China) and embedded in the paraffin wax (Solarbio, China) followed by slicing. After deparaffinization using xylene (Solarbio, China) and rehydration by ethanol (Solarbio, China). The primary antibody against Ki-67 (Beyotime, China) was applied and incubated at 4°C for 16 h. The DAB Histostaining kit (Solarbio, China) was selected to localize Ki-67 in clinical tissues.

Irisin was purchased from Cayman Chemical Company. LPS (Escherichia coli 0111: B4) was purchased from MilliporeSigma. MCC950 (cat. no. HY-12815) and 17-N-allylamino-17-demethoxygeldanamycin (17-AAG, cat. no. M2320-01) were purchased from MedChemExpress. The immunohistochemistry kit (cat. no. PV-9000) was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. Ethanol and dimethyl benzene were purchased from Tianjin Yongda Chemical Reagent Co., Ltd. The hematoxylin and eosin (H&E) Stain Kit (cat. no. G1120) and paraffin wax were purchased from Beijing Solarbio Science & Technology Co., Ltd. Primers used for reverse transcription-quantitative PCR (RT-qPCR) primers were synthesized by Sangon Biotech Co., Ltd.