Mouse albumin elisa quantitation kit

Creatinine in urine and blood was determined using a colorimetric assay (ABX Pentra Creatinine 120 CP kit, Horiba ABX SAS, Montpellier, France) according to the manufacturer’s instructions. Na⁺ and K⁺ were determined by flame photometry (model IL 943, Instrumentation Laboratory, Lexington, MA). Albumin was measured according to the manufacturer’s instructions using the Mouse Albumin ELISA Quantitation kit (Bethyl Laboratories, Inc., Montgomery, USA).

The level of Dkk-3 from HK-2 culture medium was measured by a commercial ELISA kit (Abcam). Mouse urinary albumin was measured by mouse albumin ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). Urine creatinine and serum BUN were quantified by enzymatic assays (Stanbio Laboratory, Boerne, TX, USA).

Medium was collected from EBs grown on collagen gel at indicated time points and assessed for albumin secretion with a mouse albumin ELISA quantitation kit (Bethyl). The plate was read at 450 nm for TMB using a microplate reader (Mithras) as previously described[16 (link)].

Hepatocyte conditioned media (CM) were collected on
days 3 and 5 from the different groups. We evaluated Alb
production using a Mouse Albumin ELISA Quantitation Kit
(Bethyl Laboratories, USA) and urea secretion with a Urea
Assay Kit (Biorex, UK). The values were normalized to the
total protein acquired from a Total Protein Kit (Biorex, UK)
that used an auto analyzer (Alpha-Classic, Iran).

Urine albumin levels were measured using mouse albumin ELISA quantitation kit (Bethyl Laboratories, Montgomery, TX, USA). Urine creatinine was determined by a creatinine assay kit (BioAssay Systems, Hayward, CA, USA). To rule out the influence of glomerular filtration rate on urinary excretion of albumin, urine albumin levels were adjusted by urine creatinine levels and presented as urine albumin-to-creatinine ratios.

Urine from the mice was collected into siliconized tubes. For the albumin assay, the urine was diluted 1:1,000 and measured in duplicate with the Mouse Albumin ELISA Quantitation Kit (Bethyl Laboratories Inc., USA). Optical density values were obtained using the MRX microplate reader (Dynex Technologies Inc., USA) at a wavelength of 450 nm. Albumin values (ng/ml) were determined as the mean of duplicate values. The urine creatinine (mg/dl) was measured using the alkaline picrate (Jaffé) method. Creatinine LiquiColor Test was purchase from StanBio Lab Inc. (USA). Urine creatinine assay was performed on spectrophotometer (Bio-Rad, capable of absorbance readings at 510 nm) in accordance with the manual steps. The urinary albumin/creatinine ratio (mg/g) was calculated for each animal.