Ethylenediaminetetraacetic acid edta disodium dihydrate

Carbon-coated magnetic (CCM) beads were purchased from Turbo beads (Zurich, Switzerland). Information about the synthesis and chemistry of the beads can be found elsewhere [20 (link)]. Penicillin-streptomycin was purchased from Gibco Life Technologies (Carlsbad, CA, USA). Sodium chloride (NaCl), magnesium chloride (MgCl₂), phosphate-buffered saline pH 7.4 (PBS), tris(hydroxymethyl)aminomethane (tris-base), boric acid, ethylenediaminetetraacetic acid (EDTA) disodium dihydrate, sodium azide, and hydrochloric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA; https://www.sigmaaldrich.com/united-states.html (accessed on 10 February 2021)). The size exclusion chromatographic column was obtained from Izon Science Ltd. (Lyon, France). Amicon Ultra Centrifugal Filter (0.5 mL) was supplied from EMD Millipore (Sigma, Burlington, MA, USA; https://www.merckmillipore.com (accessed on 10 May 2020)). DNA purification kit (Qiagen, Hilden, Germany), RT2 first strand kit (Qiagen, cat No 330401), and purified labeled and unlabeled oligonucleotides were purchased from Metabion International (Planegg, Germany; http://www.metabion.com (accessed on 10 May 2021)), as shown in Table 1 and Table 2. Luciferase-expressing 4T1 murine breast cancer cells (4T1-Luc2) were purchased from PerkinElmer and cultured as reported previously [21 (link)].

2,2-Diphenyl-1-picryhydrazyl (DPPH) radical, 2,2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), folin-Ciocalteu reagent, gallic acid, quercetin, aluminum chloride anhydrous, 2-thiobarbituric acid (TBA), deoxyribose, ethylenediaminetetraacetic acid (EDTA) disodium dihydrate, ascorbic acid, alamar blue and trolox were procured from Sigma–Aldrich (Sigma Aldrich India Pvt Ltd., Bangalore, India). Potassium persulphate and sodium bicarbonate were purchased from Sisco Research Laboratories Pvt. Ltd. (Maharashtra, India). Ferric chloride, hydrogen peroxide, orthophosphoric acid were purchased from Fischer Scientific (Mumbai India). All the solvents methanol, acetone, hexane, ethyl acetate, dichloromethane, and dimethyl sulphoxide were purchased from Merck Millipore (KGaA, Darmstadt, Germany). Chemicals and solvents used were of analytical and HPLC grade, respectively.