Balb cj mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

BALB/cJ mice are an inbred strain of laboratory mice commonly used in scientific research. These mice are characterized by their light-colored fur and are known for their susceptibility to certain diseases and immune responses. BALB/cJ mice are often used as a model organism in immunology, cancer, and infectious disease studies.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (15 mentions) C57bl 6j mice, by Jackson ImmunoResearch (10 mentions) C57bl 6 mice, by Jackson ImmunoResearch (5 mentions) Laboratory autoclavable rodent diet, by Land O'Lakes (3 mentions) Sigma adjuvant system, by Jackson ImmunoResearch (3 mentions) Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (2 mentions) T75 flask, by Corning (2 mentions) Sigma adjuvant system, by Merck Group (2 mentions) Complete ultra, by Roche (2 mentions) In vivo xtreme imaging system, by Bruker (2 mentions) Surgipath decalcifier 1, by Leica Biosystems (2 mentions) Fcrn mice, by Jackson ImmunoResearch (2 mentions) Omni mixer, by Omni International (2 mentions) Complete freund s adjuvant, by BD (2 mentions) Inveon μpet ct scanner, by Siemens (2 mentions) Elisa assay, by R&D Systems (2 mentions) Teklad lm 485 mouse rat sterilizable diet, by Inotiv (2 mentions) Balb canncrl mice, by Charles River Laboratories (2 mentions) Cell strainer, by Thermo Fisher Scientific (1 mentions) Ripa lysis buffer, by Merck Group (1 mentions)

243 protocols using balb cj mice

Evaluating Antitubercular Compounds in Mouse Models

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Six to eight-week-old BALB/cJ mice (Jackson Laboratories) were infected with 1000 CFU of Erdman Mtb intranasally. C3HeB/FeJ (Kramnik) mice (Jackson Laboratories) were infected with 500 CFU of Erdman Mtb intranasally. At weeks 4 through 8 post-infection, compounds or vehicle controls were administered once-daily by oral gavage. For aerosol infection, BALB/cJ mice (Jackson Laboratories) were infected via an aerosol inhalation exposure system (Glass-Col) with a calibrated dose of 200 CFU of Erdman Mtb. Experimental compounds or vehicle control were administered once-daily at weeks 4 through 8 post-infection by oral gavage. After treatment, lung tissues were collected and processed for histology and CFU enumeration. For CFUs, lungs were homogenized in PBS, 0.05% Tween-80 and plated on 7H10 OADC. Inflammatory area was scored by measuring the percent of tissue inflamed (granulomatous tissue with peripheral and peribronchial lymphocytes and plasma cells) per low power microscopic field. In a blinded fashion, 4–15 fields were analyzed per lung sample.

Wilburn K.M., Montague C.R., Qin B., Woods A.K., Love M.S., McNamara C.W., Schultz P.G., Southard T.L., Huang L., Petrassi H.M, & VanderVen B.C. (2022). Pharmacological and genetic activation of cAMP synthesis disrupts cholesterol utilization in Mycobacterium tuberculosis. PLoS Pathogens, 18(2), e1009862.

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Murine Tumor Models for Cancer Immunotherapy

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For the breast cancer model, 1 × 10⁵ 4T1 cells in 50 μl of PBS were injected into the mammary fat pad of 6–7 week old female Balbc/J mice (Jackson Laboratories) under isofluorane or ketamine anesthetic along with analgesics. For the colorectal cancer model, 2 × 10⁵ CT26 cells in 100 μl of PBS were injected into the left flanks subcutaneously of 6–7 week old female Balbc/J mice (Jackson Laboratories). Mice with clinically palpable tumors (2 mm in diameter) were randomized into indicated treatment arms. IgG (2A3, Bioxcel, 100μg), anti-Pd-1 (RMP1–14, Bioxcel 100μg), or anti-Ctla4 (9H10, Bioxcel 250μg), antibodies were administered intraperitoneally in 100 μl of PBS twice weekly (q3–4 days). Tumor volumes were measured twice weekly using calipers and calculated by the formula:

\frac{(L e n g t h) x {(W i d t h)}^{2}}{2}

. All mouse experiments shown were repeated at least twice to ensure reproducibility. Mice were euthanized by carbon dioxide prior to necropsy.

Samstein R.M., Krishna C., Ma X., Pei X., Lee K.W., Makarov V., Kuo F., Chung J., Srivastava R.M., Purohit T.A., Hoen D.R., Mandal R., Setton J., Wu W., Shah R., Qeriqi B., Chang Q., Kendall S., Braunstein L., Weigelt B., Carrillo Albornoz P.B., Morris L.G., Mandelker D.L., Reis-Filho J.S., de Stanchina E., Powell S.N., Chan T.A, & Riaz N. (2020). Mutations in BRCA1 and BRCA2 differentially affect the tumor microenvironment and response to checkpoint blockade immunotherapy. Nature cancer, 1(12), 1188-1203.

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Folic Acid Deficiency in BALB/cJ Mice

Cited 1 time

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Unless otherwise specified, studies used male BALB/cJ mice (age 24 or 28 days) bred in-house (St. Jude Children’s Research Hospital, Memphis, TN; the original breeding pairs were from Jackson Laboratories). Several experiments included BALB/cJ mice directly shipped from Jackson Laboratories (Bar Harbor, ME), or male BALB/cAnNHsd mice (age 24 or 28 days) obtained from Harlan Laboratories (Houston, TX). An irradiated folic-acid deficient diet (purchased from TestDiet, Richmond, IN) containing less than 0.05 ppm folic acid was used [17 (link)]. Prior to the initiation of all experiments, both in-house bred and vendor-derived mice were transferred into a dedicated experimental room where they were maintained in sterile micro-isolator cages (Micro Vent System 75 JAG, Allentown, NJ) and housed on ventilated racks, with up to 5 mice in a cage with corncob bedding (Andersons Bed-O’cobs, Pharmaserv, Framingham, MA). The mice had access to food and water ad libitum.

Liu C., Janke L.J., Kawedia J.D., Ramsey L.B., Cai X., Mattano LA J.r., Boyd K.L., Funk A.J, & Relling M.V. (2016). Asparaginase Potentiates Glucocorticoid-Induced Osteonecrosis in a Mouse Model. PLoS ONE, 11(3), e0151433.

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Kidney Protein Extraction from Mice

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Kidneys were obtained from 5-month-old BALB/cJ mice (Jackson Laboratory). A total of 20 mice were killed with CO₂, their blood was removed through heart puncture, and their kidneys were collected immediately. The kidneys were cleaned by rinsing with phosphate buffered saline (PBS, pH 7.2) twice and then stored at 4°C for 1 hour, -20°C for 2 hours, and then -80°C until further processing. Thawed kidneys were cut to small pieces and pressed through a cell strainer (Fisher Scientific). To remove red blood cells (RBC), kidney tissue was mixed with 10 mL of RBC lysis buffer for 10 seconds. After centrifugation for 5 minutes, the supernatant was discarded. The tissue was mixed with 40 mL of RIPA lysis buffer (Sigma-Aldrich) and 4 tablets of protease inhibitor (complete protease inhibitor cocktail, Sigma-Aldrich). The tissue mixture was sonicated for 10 minutes, or until all tissue pieces appeared dissolved. The mixture was centrifuged at 13,300 rpm for 20 minutes, and the supernatant that contained all the soluble kidney proteins was collected. Protein concentration was measured by the RC DC protein assay (Bio-Rad).

Zhang W., Rho J.H., Roehrl M.W., Roehrl M.H, & Wang J.Y. (2019). A repertoire of 124 potential autoantigens for autoimmune kidney diseases identified by dermatan sulfate affinity enrichment of kidney tissue proteins. PLoS ONE, 14(6), e0219018.

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Gait Analysis of Transgenic Mice

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We used 3- to 4-month-old male Balb/cJ mice, weighing 20-30 g. Animals were purchased by Jackson Lab (Bar Harbor, ME, USA), bred in our facilities and genotyped as reported by Campbell et al (1999) (link). Because the pattern of inheritance of D1CT-7 mice is autosomal dominant, wild-type (WT) females were bred with heterozygous D1CT-7 sires; this breeding scheme was selected to standardize maternal behavior. Animals were housed in group cages with ad libitum access to food and water. The room was maintained at 22 C, on a 12:12 h light/dark cycle from 0800 to 2000 h. Mice were tested during their light cycle, between 1200 and 1600h, to minimize potential circadian effects. The mice used for gait analysis were the same animals used for the locomotor activity section of the paper by Godar et al. (2016) (link). All experimental procedures were in compliance with the National Institute of Health guidelines and approved by the Institutional Animal Use Committees of the Universities of Kansas and Utah.

Fowler S.C., Mosher L.J., Godar S.C, & Bortolato M. (2017). Assessment of gait and sensorimotor deficits in the D1CT-7 mouse model of Tourette syndrome. Journal of neuroscience methods, 292, 37-44.

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BALB/cJ Mice Welfare Protocol

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All animals used in this study were female BALB/cJ mice (The Jackson Laboratory, Bar Harbor, ME) of 3–8 months of age. Mice had ad libitum access to food and water, and were maintained in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The local Institutional Animal Care and Use Committee (Feinstein Institute for Medical Research) approved the animal protocol. All efforts were made to minimize and ameliorate suffering and pain to animals used in this study.

Chang E.H., Carreiro S.T., Frattini S.A, & Huerta P.T. (2019). Assessment of glutamatergic synaptic transmission and plasticity in brain slices: relevance to bioelectronic approaches. Bioelectronic Medicine, 5, 6.

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Respiratory Syncytial Virus Infection in Mice

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Balb/cJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME) at 6-8 weeks of age and bred in-house, as previously described²⁴. All mice were maintained in a pathogen-free facility at the University of Pittsburgh, Division of Laboratory Animal Resources (Pittsburgh, PA) and handled according to protocols approved by The University of Pittsburgh Institutional Animal Care and Use Committee. Mice were infected intranasally (i.n.) with 5 × 10⁵ plaque forming units (pfu) of RSV Line 19 (RSV L19, a gift from Martin Moore, Emory University) per gram of body weight at 4-5 PND (infants, ~1.5 × 10⁶ pfu in 10 μl) or 6-8 weeks of age (adults, ~10⁷ pfu in 100 μl) under isoflurane anesthesia. The RSV L19 was propagated and viral titers were quantified as previously described^{25 (link)}. Recombinant murine IFN-γ (16ng/g) (Peprotech, Rocky Hill, NJ) or vehicle (PBS) were administered i.n. to infants on 1, 3 and 5 days post-infection (DPI) under isoflurane anesthesia.

Eichinger K.M., Kosanovich J.L, & Empey K.M. (2017). Localization of the T-Cell Response to RSV Infection is Altered in Infant Mice. Pediatric pulmonology, 53(2), 145-153.

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Murine Xenograft Tumor Modeling

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For in vivo experiments, we used 6- to 12-week-old female nonobese diabetic severe combined immunodeficiency, interleukin 2 receptor gamma chain deficient, NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ (NSG) mice or BALB/cJ mice (The Jackson Laboratory, Bar Harbor, ME, USA). Animals were maintained in a specific pathogen-free facility at the University of British Columbia (UBC) Biomedical Research Centre. All experiments were conducted with approval of the UBC Animal Care Committee.
Primary tumor development was examined following subcutaneous (s.c.) injection of MDA-MB-231 cells (1 × 10⁶) prepared in BD Matrigel™ (BD Biosciences, San Jose, CA, USA) into the right hind flank of NSG mice. Tumor growth was measured using manual calipers, and the tumor volume was estimated using the following formula: length times width² divided by 2. Final tumor masses were measured after excision and the tumors were retained for histochemical analyses. Flow cytometry was performed on lung digests to enumerate tumor cells based on detection of GFP or RFP fluorescence.

Snyder K.A., Hughes M.R., Hedberg B., Brandon J., Hernaez D.C., Bergqvist P., Cruz F., Po K., Graves M.L., Turvey M.E., Nielsen J.S., Wilkins J.A., McColl S.R., Babcook J.S., Roskelley C.D, & McNagny K.M. (2015). Podocalyxin enhances breast tumor growth and metastasis and is a target for monoclonal antibody therapy. Breast Cancer Research : BCR, 17(1), 46.

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Mouse Strains for Immunology Research

Cited 2 times

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Female and male mice were bred and housed by the Biomedical Research Unit at the Malaghan Institute of Medical Research. All animal experiments were performed in accordance with relevant guidelines and regulations and were approved by Victoria University of Wellington animal ethics committee. Animals used included: C57BL/6J (originally from The Jackson Laboratory, Bar Harbor, ME, USA) and the CD45.1 congenic strain B6.SJL-Ptprca Pepc^b/BoyJ (from Ozgene Pty, Bentley, WA, Australia), BALB/cJ mice (The Jackson Laboratory), Cd1d^−/− mice^{33 (link)} and Traj18^−/− mice (B6(Cg)-Traj18^tm1.1Kro/J; The Jackson Laboratory).^{34 (link)}Ifnar1^flox/flox mice^{35 (link)} were crossed with CD11c-cre mice (Tg(Itgax-cre,-EGFP)4097Ach), and then an F2 cross performed to give cre-positive Ifnar1^ΔCD11c mice and cre-negative littermates used as controls (Ifnar1^+/+). Experiments in Clec9a-DTR mice, which express the human diphtheria toxin (DT) receptor under the control of the Clec9a promoter,^{36 (link)} and Siglec-H-DTR mice which express the human DT receptor under the control of the Siglec-H promoter,^{36 (link)} were conducted in F1 crosses with C57BL/6J mice. Both were supplied by Nanyang Technological Unit, Singapore.

Prasit K.K., Ferrer-Font L., Burn O.K., Anderson R.J., Compton B.J., Schmidt A.J., Mayer J.U., Chen C.J., Dasyam N., Ritchie D.S., Godfrey D.I., Mattarollo S.R., Dunbar P.R., Painter G.F, & Hermans I.F. (2022). Intratumoural administration of an NKT cell agonist with CpG promotes NKT cell infiltration associated with an enhanced antitumour response and abscopal effect. Oncoimmunology, 11(1), 2081009.

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T Cell-Specific gp96 Deletion in Mice

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T cell–specific deletion of gp96 on a C57BL/6J background was accomplished by crossing Hsp90b1^{flox/flox (16 (link))} mice with Cd4-Cre (Tg(Cd4-cre)1Cwi/BfluJ) transgenic mice (The Jackson Laboratory). C57BL/6J, Rag1^−/− (B6.129S7-Rag1^tm1Mom/J), OT-1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J), TRP-1 (B6.Cg-Rag1^tm1Mom Tyrp1^B-w Tg(Tcra,Tcrb)9Rest/J), or BALB/cJ mice were purchased from Jackson Laboratories. Ly5.2 (B6.SJL-Ptprc^a Pepc^b/BoyJ) mice were a kind gift of Dr. Xue-Zhong Yu. All animal experiments were approved Medical University of South Carolina (MUSC) Institutional Animal Care and Use Committee and the Division of Laboratory Animal Resources at MUSC maintained all mice.

Thaxton J.E., Wallace C., Riesenberg B., Zhang Y., Paulos C., Beeson C., Liu B, & Li Z. (2017). Modulation of Endoplasmic Reticulum Stress Controls CD4+ T Cell Activation and Anti-tumor Function. Cancer immunology research, 5(8), 666-675.

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