Temozolomide (TMZ) and Sulfasalazine (SAS) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Erastin was purchased from Hycultec GmbH (Beutelsbach, Germany). Sorafenib was purchased from LC Laboratories (Woburn, USA). S-4-Carboxyphenylglycine was purchased from ACRIS Antibodies (Herford, Germany). Temozolomid was solved under sterile conditions in dimethylsulphoxide (DMSO) to concentration of 300 mM. Sulfasalazine was dissolved in 400 mM ammonium hydroxide under sterile conditions to concentration of 200 mM. Erastin and Sorafenib were dissolved in DMSO under sterile conditions to concentration of 100 mM. S-4-CPG was solved under sterile conditions in 1 M sodium hydroxide to concentration of 100 mM. Deferoxamine (DFO) and Ferrostatin-1 (Ferr-1) were purchased from Sigma-Aldrich (Taufkirchen, Germany). Deferoxamine was dissolved in water under sterile conditions to a concentration of 50 mM. Ferrostatin-1 was prepared in 50% DMSO/water under sterile conditions to a concentration of 50 mM.
Sulfasalazine
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy
Sulfasalazine is a laboratory compound used as a chemical reagent. It is a yellow, crystalline powder that is commonly utilized in various analytical and research applications. The core function of Sulfasalazine is to serve as a chemical standard or reference material in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Erastin, by Selleck Chemicals (7 mentions)
Ferrostatin 1, by Merck Group (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
N acetyl cysteine (nac), by Merck Group (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
L glutamine, by Thermo Fisher Scientific (4 mentions)
L cystine, by Merck Group (4 mentions)
Cisplatin, by Merck Group (4 mentions)
Ml162, by Cayman Chemical (4 mentions)
Deferoxamine, by Merck Group (4 mentions)
2 mercaptoethanol, by Merck Group (4 mentions)
Trolox, by Merck Group (4 mentions)
Erastin, by Merck Group (4 mentions)
Ko143, by Merck Group (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Methionine, by Thermo Fisher Scientific (3 mentions)
L methionine, by Merck Group (3 mentions)
N acetyl l cysteine, by Merck Group (3 mentions)
D penicillamine, by Merck Group (3 mentions)
L penicillamine, by Merck Group (3 mentions)
95 protocols using sulfasalazine
1
Preparation of Small Molecule Compounds
2
Reagents for Cell Death Assays
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Deferoxamine, ferrostatin-1, erastin, sulfacetamide, sulfasalazine (SSZ), zymosan, diphenyleneiodonium chloride (DPI), 4,4′-diaminodiphenyl sulfone (DDS), sulfisoxazole, tBHP, and sulfasalazine were purchased from Sigma. Phorbol 12-myristate 13-acetate (PMA), sulfamethoxazole, sulfanilamide, sulfapyridine, 5-aminosalicylic acid (5-ASA), sulfadimidine, sulfametoxydiazine, sulfisomidine, sulfanitran, sulfanilamide, and piroxicam were purchased from Wako. We purchased 1-O-hexadecyl-2-arachidonoyl-sn-glycero-3-phosphocholine (ether-linked phosphatidylcholine) from Avanti polar lipids. Necrostatin-1 was purchased from FOCUS Biomolecules, and 2-mercaptoethanol was purchased from MP Biomedicals. z-VAD-fmk was purchased from Peptide institute. Trolox, sulfadiazine, sulfadimethoxine, sulfadoxin, and sulfamethoxypyridazine were purchased from Tokyo Chemical Industry. Cl-amidine was purchased from Cayman chemicals. Ionomycin was purchased from Merck Millipore.
3
Myelinating Glia Ablation and Sulfasalazine Treatment
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Metronidazole (Mtz, M1547, Sigma) was dissolved in EM containing 0.2% DMSO to 10 mM as previously described (Chung et al., 2013 (link)). For the ablation of myelinating glia, Tg(mbp:gal4-vp16;uas:nfsB-mcherry) and Tg(claudink:gal4-vp16;uas:nfsB-mcherry) larvae were incubated in 10 mM Mtz for 72 h starting at 5 dpf. For sulfasalazine (Sigma) treatment, transgenic larvae were rinsed at 7 dpf in embryo medium (EM; 15 mM NaCl, 0.5 mM KCl, 1 mM CaCl2, 1 mM MgSO4, 0.15 mM KH2PO4, 0.05 mM NH2PO4, 0.7 mM NaHCO3) and incubated in EM containing 10 uM sulfasalazine for 1 day.
4
Labeling and Conjugation of APR-246 Compounds
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APR‐246 (2‐hydroxymethyl‐2‐methoxymethyl‐1‐azabicyclo[2,2,2]octan‐3‐one), 14C‐labeled APR‐246 and glutathione‐conjugated MQ (GS‐MQ) were obtained from Aprea Therapeutics AB (Stockholm, Sweden). APR‐246 was dissolved in either DMSO or water. MK‐571 (final concentration to cells 5–20 μM) and Sulfasalazine (final concentration to cells 100–200 μM) were from Merck (Germany). MK‐571 for treatment of esophageal cancer cells in vitro and in vivo was from Selleckchem (Houston, TX) or for colo‐PDOs from Cayman Chemical (USA) in 0.9% saline. Reversan (final concentration to cells 10–20 μM) was from Santa Cruz Biotechnology, sc‐296262 (USA). Inhibitors were dissolved in DMSO. The percentage of DMSO during drug exposure never exceeded 0.2% for cell lines and 0.5% for eso‐PDOs. Doxycycline and chemicals described hereafter were all from Merck (Germany).
5
TNBS-Induced Colitis Model
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2,4,6-trinitrobenzene sulfonic acid (TNBS), carboxymethyl cellulose (CMC), sulfasalazine, and ethanol were purchased from Merck chemical company (Germany). Shiraz University of Medical Sciences (SUMS) supplied the laboratory facilities, including enzyme-linked immunosorbent assay (ELISA) kits for detecting TNF-α, IL-6, and NO. All other chemicals reached analytical grade.
6
Investigating Cell Line Responses to Small Molecules
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Huh7 and SK-HEP-1 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). Dulbecco’s modified Eagle medium, RPMI-1640 medium, fetal bovine serum, L-glutamine, penicillin, and streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Sulfasalazine, ferrostatin-1, necrostatin-1, deferoxamine, and 1-methyl-D-tryptophan were purchased from Merck KGaA (Darmstadt, Germany). Erastin, RSL3, necrostatin-1s, and GSK2982772 and Z-VAD-FMK were obtained from Selleckchem (Houston, TX, USA). TNFα was supplied from PeproTech (Cranbury, NJ, USA) and SM164 was obtained from AdooQ Bioscience (Irvine, CA, USA).
7
Ferroptosis Pathway Screening in Liver Cancer Cells
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Huh6, Huh7, and SK-HEP-1 cells were obtained from the Korean Cell Line Bank (Seoul, Korea). DMEM with no glutamine, methionine, RPMI with no glutamine, methionine, and L-cystine, fetal bovine serum, antibiotics, and L-glutamine were obtained from Thermo Fisher Scientific (Waltham, MA, USA). L-methionine, L-cystine, sulfasalazine, and propidium iodide (PI) were purchased from Merck (Darmstadt, Germany). Erastin and RSL3 were supplied by Selleckchem (Houston, TX, USA).
8
Microbial Metabolism of Diverse Drugs
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The following drugs were obtained from Pfizer (Groton, CT): omeprazole, simvastatin, metronidazole, risperidone, sulfinpyrazone, sulindac, levodopa, dapsone, nizatidine, and acetaminophen (Table 1). Zonisamide and sulfasalazine were obtained from Sigma-Aldrich (St. Louis, MO). The drugs were selected based on their BCS classification, chemical structure, widespread prescription, and/or previously reported metabolism detected in human or animal feces. All drugs were diluted in dimethylsulfoxide (DMSO) and were incubated with human colon microbiota using the fermentation screening platform, followed by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and highperformance liquid chromatography (HPLC) analysis.
9
Establishment of Cisplatin-Resistant GC Cell Lines
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The primary human GC cell lines HEK293T and SGC7901 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and grown in DMEM (Gibco BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (Gibco BRL, Gaithersburg, MD, USA) and antibiotics (100 IU/ml penicillin and 100 μg/ml streptomycin). The Cisplatin-resistant cell subline SGC7901/DDP was generated from the sensitive parental GC cell line SGC7901 in our laboratory. Cells were grown in increasing Cisplatin concentrations (the nal concentration of DDP was 1 μg/ml) for 4 months. Cisplatin (DDP; Sigma, Inc., St. Louis, MO, USA; 1 μg/ml) was added to the culture medium of appropriate cell sublines to maintain the DDP-resistant phenotype. Cisplatin and sulfasalazine were purchased from Sigma-Aldrich (St. Louis, MO, USA).
10
Compound Procurement for Research
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BZ was obtained from Selleckchem (Munich, Germany), Sulfasalazine and Glu from Sigma-Aldrich (Darmstadt, Germany) and (RS)α-Cyclopropyl-4-phosphonophenylglycine (CPPG) was obtained from Tocris Bioscience (Bristol, UK).
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