Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences Inc., San Diego, CA, USA) according to the manufacturer's instructions. Briefly, ADSCs (1 × 105 cells) were mixed into 0.5 ml of Perfusion Solution (CORNING, Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell mixture and incubated on ice for 30 minutes. After washing the cells with Brilliant Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence-activated cell sorting (FACS) measurements were conducted. The following primary antibodies were used: Brilliant Violet 421™ Rat Anti-Mouse CD44 (BD Biosciences), Fluorescein Isothiocyanate (FITC) Rat Anti-Mouse CD90.2 (BD Biosciences), PerCP/Cy5.5 Anti-Mouse CD34 (BioLegend, San Diego, CA, USA), and PE/Cy7 Rat Anti-Mouse CD45 (BD Biosciences). Isotype-identical antibodies were used as controls.
Perfusion solution
Manufactured by Corning
Sourced in United States
Perfusion Solution is a laboratory product designed for use in cell culture applications. It is a balanced salt solution that provides a nutrient-rich environment to support the growth and maintenance of cells during perfusion, a technique used to continuously supply fresh media to the cells. The solution helps maintain optimal pH, osmolality, and ionic balance to support cell viability and function during extended cell culture procedures.
Automatically generated - may contain errors
Lab products found in correlation
Novocyte flow cytometer, by Agilent Technologies (4 mentions)
Brilliant stain buffer, by BD (4 mentions)
Pe cy7 rat anti mouse cd45, by BD (2 mentions)
Fluorescein isothiocyanate fitc rat anti mouse cd90, by BD (2 mentions)
Percp cy5.5 anti mouse cd34, by BioLegend (2 mentions)
Pe cy7 mouse igg1 κ isotype ctrl antibody, by BioLegend (2 mentions)
Apc mouse anti human cd29, by BD (2 mentions)
Pe cy7 anti human cd45 antibody, by BioLegend (2 mentions)
Fitc mouse igg1 κ isotype ctrl antibody, by BioLegend (1 mentions)
4 protocols using perfusion solution
1
Phenotypic Characterization of Adipose-Derived Stem Cells
2
Flow Cytometry Analysis of hADSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. In brief, hADSCs (1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING, Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture, which was then incubated on ice for 30 min. After washing the cells with Brilliant Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), FACSs measurement was carried out. The following primary antibodies was used: APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences); FITC anti-human CD90.2 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
3
Characterization of Mouse Adipose-Derived Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell flow cytometry was performed using a NovoCyte Flow Cytometer (ACEA Biosciences, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, mAdMSCs (1 × 105) were added to 0.5 mL of perfusion solution (Corning, Manassas, VA, USA). Antibodies (1/100 dilution) were added to the cell suspensions, which were then incubated on ice for 30 min. Fluorescence-activated cell sorting was performed after washing the cells with Brilliant Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA). The primary antibodies were as follows: Brilliant Violet 421TM Rat Anti-Mouse CD44 (BD Biosciences), Fluorescein Isothiocyanate (FITC) Rat Anti-Mouse CD90.2 (BD Biosciences), PerCP/Cy5.5 Anti-Mouse CD34 (Biolegend, San Diego, CA, USA), and PE/Cy7 Rat Anti-Mouse CD45 (BD Biosciences). Isotype-specific antibodies were used as controls. Flow cytometry was performed as previously described [29 ,42 (link),43 (link)].
4
Characterization of hMSC-AT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences,
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!