H3.1 h4 tetramer

Manufactured by New England Biolabs

The H3.1–H4 tetramer is a key component of the nucleosome core particle, which is the fundamental unit of chromatin. It consists of two copies each of the histone proteins H3.1 and H4, forming a stable protein complex that plays a crucial role in the packaging and organization of DNA within eukaryotic cells.

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Lab products found in correlation

H2a h2b dimer, by New England Biolabs (2 mentions) Anti acetyl histone h3 lys9 antibody, by Merck Group (1 mentions)

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Recombinant histone H3.1–H4 tetramers (2 citations)

2 protocols using h3.1 h4 tetramer

Histone Acetylation Assay Protocol

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Chemicals were purchased from Sigma Chemical Company, USA, unless otherwise specified. Recombinant histones H1⁰, H2A, H2B, H3.3, H4, H2A–H2B dimer and H3.1–H4 tetramer were from New England Biolabs. Anti-acetyl-Histone H3 (Lys18 and Lys 27) antibodies were purchased from Abcam and anti-acetyl-Histone H3 (Lys 9) antibody was from Millipore. The secondary anti-rabbit antibody was from Abcam. Chemiluminescent substrates were from Thermo Scientific (SuperSignal West Pico Substrate) and the developer and fixer solutions were from Kodak. Histone acetyltransferase CBP and GCN5 were from Enzo Life Sciences. The commercial histones were dialyzed extensively against 10 mM Tris–HCl, pH 7.0 containing 150 mM NaCl prior to experiments.

Banerjee A., Sanyal S., Kulkarni K.K., Jana K., Roy S., Das C, & Dasgupta D. (2014). Anticancer drug mithramycin interacts with core histones: An additional mode of action of the DNA groove binder. FEBS Open Bio, 4, 987-995.

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Purification of Core Histones

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Histones were purified from HT1080 cells by acid extraction and separated by reverse phase high-pressure liquid chromatography (HPLC) (Shechter et al., 2007 (link)). Alternatively, purified recombinant human core histones, the H2A–H2B dimer, the H3.1–H4 tetramer (New England Biolabs) and mononucleosomes (EpiCypher) were purchased.

Jullien D., Vignard J., Fedor Y., Béry N., Olichon A., Crozatier M., Erard M., Cassard H., Ducommun B., Salles B, & Mirey G. (2016). Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells. Journal of Cell Science, 129(13), 2673-2683.

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