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Silencer select no 1 sirna

Manufactured by Thermo Fisher Scientific
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Silencer Select No 1 siRNA is a small interfering RNA (siRNA) product designed for gene silencing applications in cellular research. The core function of this product is to facilitate the knockdown of target gene expression through RNA interference (RNAi) mechanisms.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Silencer Select siRNAs (565 citations) Silencer Select (341 citations) SiRNAs (258 citations) Silencer siRNA (37 citations) Silencer Select #1 (20 citations) Silencer Select siRNA‐1 (2 citations) (#1 siRNA; (2 citations)

2 protocols using silencer select no 1 sirna

1

siRNA Transient Transfection Protocol

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siRNA transient transfection was carried out with the Lipofectamine® RNAiMAX (Invitrogen, Catalog number 13778150) or Lipofectamine™ 2000 (Invitrogen, Catalog number 11668019) transfection reagent according to the manufacturer’s instructions. siRNAs used in this study included siPDK1 (s10253 and s10254) (Ambion, Catalog number 4390824) and non-targeting siRNA (Ambion, Silencer select no 1 siRNA, Catalog number 4390843). Briefly, the cells were transfected with 10 nM of siRNA and incubated at 37 °C (5% CO2) for 72 h.
Subramaniam S., Jeet V., Gunter J.H., Clements J.A, & Batra J. (2021). Allele-Specific MicroRNA-Mediated Regulation of a Glycolysis Gatekeeper PDK1 in Cancer Metabolism. Cancers, 13(14), 3582.
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2

Transient gene knockdown by siRNA

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Cells were transfected with siRNAs using the Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen, Catalog number – 13778150) according to the manufacturer’s instructions and incubated at 37 °C (5% CO2) for 72 hours and transfection efficiency was confirmed by qRT-PCR analysis. siRNAs used in this study were siIRX4 (Ambion, Catalog number – AM16708, s27097 and HSS121376), siERG (Ambion, Catalog No 4392420, s4811), siAR (Dharmacon, Catalog number - DHA- J-003400-07-0010) and non-targeting siRNA (Ambion, Silencer select no 1 siRNA, Catalog number – 4390843).
Janaththani P., Tevz G., Fernando A., Malik A., Rockstroh A., Kryza T., Walpole C., Moya L., Lehman M., Nelson C., Srinivasan S., Clements J, & Batra J. (2023). Unravelling the Role of Iroquois Homeobox 4 and its Interplay with Androgen Receptor in Prostate Cancer. Research Square.
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