Streptomycin

hPDLSCs were obtained from tissues attached to the middle third of the tooth root from healthy patients 15–20 years old (five men and five women), as described previously [49 (link)], who were undergoing orthodontic treatment at the First Affiliated Hospital of Jinan University. All experimental protocols were approved by the Ethics Committee of Jinan University (Guangdong, China) (Approval number: 2019228, 28 February 2019). The tissues were minced, digested, and cultured in α-MEM (Gibco, New York, NY, USA), supplemented with 10% fetal bovine serum (FBS, Gibco), 100 mg/mL streptomycin, and 100 U/mL penicillin (MDBio, Shanghai, China) at 37 °C with 5% CO₂. The medium was changed every 3 d, and hPDLSCs at passages (P) 3–5 were used in the following experiments.

The HCC38, MDA-MB-468, MDA-MB-231, MCF7, MCF10A cells were purchased from the American Type Culture Collection (ATCC). HCC38 cells were cultured in RPMI 1640 medium (GIBCO, C11875500BT) supplemented with 10% fetal bovine serum (FBS). MCF10A cells were cultured in RPMI 1640 medium supplemented with horse serum (5%), insulin (10μg/mL) and epidermal growth factor (20 ng/mL), hydrocortisone (0.5μg/mL). MCF7, MDA-MB-231 and MDA-MB-468 cells were grown in Dulbecco’s modified Eagle’s medium (GIBCO, C11995500BT) supplemented with 10% FBS at 37 °C and 5% CO2,100 units per mL penicillin (MDbio, P003-10 g), and 100 mg/mL streptomycin (MDbio, S007-25 g).